International
Tables for
Crystallography
Volume B
Reciprocal space
Edited by U. Shmueli

International Tables for Crystallography (2006). Vol. B, ch. 2.5, pp. 276-345   | 1 | 2 |
https://doi.org/10.1107/97809553602060000558

Chapter 2.5. Electron diffraction and electron microscopy in structure determination

J. M. Cowley,a P. Goodman,b B. K. Vainshtein,c B. B. Zvyagind and D. L. Dorsete

aArizona State University, Box 871504, Department of Physics and Astronomy, Tempe, AZ 85287-1504, USA, bSchool of Physics, University of Melbourne, Parkville, Australia 3052, cInstitute of Crystallography, Academy of Sciences of Russia, Leninsky prospekt 59, Moscow B-117333, Russia, dInstitute of Ore Mineralogy (IGEM), Academy of Sciences of Russia, Staromonetny 35, 109017 Moscow, Russia, and  eExxonMobil Research and Engineering Co., 1545 Route 22 East, Clinton Township, Annandale, New Jersey 08801, USA

This chapter deals with electron diffraction and imaging in the transmission geometry at kilovolt and higher beam energies for the purposes of crystal structure determination. Techniques such as low-energy electron diffraction, gas electron diffraction and reflection electron diffraction are not considered. Following a brief review of the history of the subject and its relationship to X-ray diffraction, the fundamental theory is presented by J. M. Cowley in Section 2.5.2[link]. Unlike X-rays, which diffract from the electron density in a crystal, electrons are scattered elastically by the Coulomb potential, which is related to the density through Poisson's equation and includes the nuclear contribution. Electrons are much more strongly scattered than X-rays and have much smaller wavelengths. (This leads to a very ‚flat’ Ewald sphere and the simultaneous excitation of many Bragg beams.) Electron sources have comparable brightness to third-generation synchrotrons fitted with an undulator, so that Bragg intensities are very high. Because of their limited penetration, samples for transmission electron microscopes (TEMs) usually have thicknesses of less than a micron, while for interpretable atomic resolution images, thicknesses of tens of nanometres are preferred. Multiple scattering complicates TEM image interpretation and diffraction for larger thicknesses. The current (2006) spatial resolution of the best aberration-corrected TEM instruments is about 0.8 nm. Microdiffraction patterns may be obtained using a beam width of sub-nanometre dimensions, while the analysis of characteristic X-rays excited by such a probe provides chemical information. For organic monolayers (such as proteins), the amount of structural information obtained per unit of radiation damage (which is a strong function of resolution) exceeds that obtainable by X-ray diffraction. Cowley's treatment includes sections on electron scattering factors, Bethe's 1928 multiple scattering theory, Born's series, sign conventions, two-beam dynamical theory and single-scattering theory. This is followed by the theory of electron microscope imaging at high resolution, including the weak-phase object and other approximations, and the Scherzer focusing condition. The section ends with treatments of atomic resolution imaging in crystals and the factors which limit it, with Fourier or Talbot self-imaging, and with a brief discussion of coherent nanodiffraction. Section 2.5.3[link] by P. Goodman describes the convergent-beam electron diffraction (CBED) method used for space-group determination. This information is obtained in the microdiffraction mode, allowing nanocrystals to be analysed. A cone of illumination is used, which broadens the Bragg spots into discs, whose internal symmetries are analysed together with the symmetry of the whole pattern of discs. This section reviews the history of the subject, and provides tables which allow a systematic deduction of point and space groups from these data in most cases. Multiple scattering effects, in particular, can be used to distinguish between centrosymmetric and noncentrosymmetric crystals, while cancellation along symmetry-related multiple scattering paths allows identification of screw and glide elements. Examples from the literature are cited and sources of useful multiple-scattering software for simulating CBED patterns are noted. Section 2.5.4[link] by B. K. Vainshtein and B. B. Zvyagin is devoted to the use of transmission electron diffraction patterns to solve the structures of thin crystal structures using the kinematic theory. Texture and polycrystalline patterns are considered, as is the relationship to X-ray work. Section 2.5.5[link] by B. K. Vainshtein again outlines the theory of high-resolution electron imaging, extending this to include image processing, image cross-correlation and alignment, and image filtering and enhancement. Section 2.5.6[link] by B. K. Vainshtein discusses the problem of three-dimensional image reconstruction from projections, widely used in cryo-electron microscopy of proteins, and increasingly also now in materials science. The real-space methods of Radon, the method of back-projection, iterative methods and reciprocal-space techniques are described in full. Section 2.5.7[link] by D. L. Dorset summarizes solutions to the phase problem which may be applied to electron diffraction data. These include many of those currently in use for X-ray diffraction, including Patterson maps, direct methods and trial-and-error search techniques. Much of the section concerns electron diffraction data from thin organic films, analysed using the three-phase invariants of the direct methods approach to phasing. The tangent formula is reviewed and useful software is suggested. Density modification and maximum entropy methods are outlined. Because of the strong dependence of multiply scattered electron diffraction intensities on the thickness of the sample, and on local orientation changes (effects which are not accounted for in the structure analysis of the data), the thinnest possible samples must be used, and phasing measures are sought which are robust with respect to multiple scattering perturbations. Conversely, the ability to obtain data from nanometre-sized regions can greatly assist the effort to obtain high-quality perfect-crystal data free of defects, bending or thickness variation.

2.5.1. Foreword1

| top | pdf |
J. M. Cowleya

Given that electrons have wave properties and the wavelengths lie in a suitable range, the diffraction of electrons by matter is completely analogous to the diffraction of X-rays. While for X-rays the scattering function is the electron-density distribution, for electrons it is the potential distribution which is similarly peaked at the atomic sites. Hence, in principle, electron diffraction may be used as the basis for crystal structure determination. In practice it is used much less widely than X-ray diffraction for the determination of crystal structures but is receiving increasing attention as a means for obtaining structural information not readily accessible with X-ray- or neutron-diffraction techniques.

Electrons having wavelengths comparable with those of the X-rays commonly used in diffraction experiments have energies of the order of 100 eV. For such electrons, the interactions with matter are so strong that they can penetrate only a few layers of atoms on the surfaces of solids. They are used extensively for the study of surface structures by low-energy electron diffraction (LEED) and associated techniques. These techniques are not covered in this series of volumes, which include the principles and practice of only those diffraction and imaging techniques making use of high-energy electrons, having energies in the range of 20 keV to 1 MeV or more, in transmission through thin specimens.

For the most commonly used energy ranges of high-energy electrons, 100 to 400 keV, the wavelengths are about 50 times smaller than for X-rays. Hence the scattering angles are much smaller, of the order of [10^{-2}] rad, the recording geometry is relatively simple and the diffraction pattern represents, to a useful first approximation, a planar section of reciprocal space.

The elastic scattering of electrons by atoms is several orders of magnitude greater than for X-rays. This fact has profound consequences, which in some cases are highly favourable and in other cases are serious hindrances to structure analysis work. On the one hand it implies that electron-diffraction patterns can be obtained from very small single-crystal regions having thicknesses equal to only a few layers of atoms and, with recently developed techniques, having diameters equivalent to only a few interatomic distances. Hence single-crystal patterns can be obtained from microcrystalline phases.

However, the strong scattering of electrons implies that the simple kinematical single-scattering approximation, on which most X-ray diffraction structure analysis is based, fails for electrons except for very thin crystals composed of light-atom materials. Strong dynamical diffraction effects occur for crystals which may be 100 Å thick, or less for heavy-atom materials. As a consequence, the theory of dynamical diffraction for electrons has been well developed, particularly for the particular special diffracting conditions relevant to the transmission of fast electrons (see Chapter 5.2[link] ), and observations of dynamical diffraction effects are commonly made and quantitatively interpreted. The possibility has thus arisen of using the observation of dynamical diffraction effects as the basis for obtaining crystal structure information. The fact that dynamical diffraction is dependent on the relative phases of the diffracted waves then implies that relative phase information can be deduced from the diffraction intensities and the limitations of kinematical diffraction, such as Friedel's law, do not apply. The most immediately practicable method for making use of this possibility is convergent-beam electron diffraction (CBED) as described in Section 2.5.3[link].

A further important factor, determining the methods for observing electron diffraction, is that, being charged particles, electrons can be focused by electromagnetic lenses. The irreducible aberrations of cylindrical magnetic lenses have, to date, limited the resolution of electron microscopes to the extent that the least resolvable distances (or `resolutions') are about 100 times the electron wavelength. However, with microscopes having a resolution of better than 2 Å it is possible to distinguish the individual rows of atoms, parallel to the incident electron beam, in the principal orientations of many crystalline phases. Thus `structure images' can be obtained, sometimes showing direct representation of projections of crystal structures [see IT C (2004[link]), Section 4.3.8[link] ]. However, the complications of dynamical scattering and of the coherent imaging processes are such that the image intensities vary strongly with crystal thickness and tilt, and with the defocus or other parameters of the imaging system, making the interpretation of images difficult except in special circumstances. Fortunately, computer programs are readily available whereby image intensities can be calculated for model structures [see IT C (2004[link]), Section 4.3.6[link] ] Hence the means exist for deriving the projection of the structure if only by a process of trial and error and not, as would be desirable, from a direct interpretation of the observations.

The accuracy with which the projection of a structure can be deduced from an image, or series of images, improves as the resolution of the microscope improves but is not at all comparable with the accuracy attainable with X-ray diffraction methods. A particular virtue of high-resolution electron microscopy as a structural tool is that it may give information on individual small regions of the sample. Structures can be determined of `phases' existing over distances of only a few unit cells and the defects and local disorders can be examined, one by one.

The observation of electron-diffraction patterns forms an essential part of the technique of structure imaging in high-resolution electron microscopy, because the diffraction patterns are used to align the crystals to appropriate axial orientations. More generally, for all electron microscopy of crystalline materials the image interpretation depends on knowledge of the diffraction conditions. Fortunately, the diffraction pattern and image of any specimen region can be obtained in rapid succession by a simple switching of lens currents. The ready comparison of the image and diffraction data has become an essential component of the electron microscopy of crystalline materials but has also been of fundamental importance for the development of electron-diffraction theory and techniques.

The individual specimen regions giving single-crystal electron-diffraction patterns are, with few exceptions, so small that they can be seen only by use of an electron microscope. Hence, historically, it was only after electron microscopes were commonly available that the direct correlations of diffraction intensities with crystal size and shape could be made, and a proper basis was available for the development of the adequate dynamical diffraction theory.

For the complete description of a diffraction pattern or image intensities obtained with electrons, it is necessary to include the effects of inelastic scattering as well as elastic scattering. In contrast to the X-ray diffraction case, the inelastic scattering does not produce just a broad and generally negligible background. The average energy loss for an inelastic scattering event is about 20 eV, which is small compared with the energy of about 100 keV for the incident electrons. The inelastically scattered electrons have a narrow angular distribution and are diffracted in much the same way as the incident or elastically scattered electrons in a crystal. They therefore produce a highly modulated contribution to the diffraction pattern, strongly peaked about the Bragg spot positions (see Chapter 4.3[link] ). Also, as a result of the inelastic scattering processes, including thermal diffuse scattering, an effective absorption function must be added in the calculation of intensities for elastically scattered electrons.

The inelastic scattering processes in themselves give information about the specimen in that they provide a measure of the excitations of both the valence-shell and the inner-shell electrons of the solid. The inner-shell electron excitations are characteristic of the type of atom, so that microanalysis of small volumes of specimen material (a few hundreds or thousands of atoms) may be achieved by detecting either the energy losses of the transmitted electrons or the emission of the characteristic X-ray [see IT C (2004[link]), Section 4.3.4[link] ].

An adverse effect of the inelastic scattering processes, however, is that the transfer of energy to the specimen material results in radiation damage; this is a serious limitation of the application of electron-scattering methods to radiation-sensitive materials such as organic, biological and many inorganic compounds. The amount of radiation damage increases rapidly as the amount of information per unit volume, derived from the elastic scattering, is increased, i.e. as the microscope resolution is improved or as the specimen volume irradiated during a diffraction experiment is decreased. At the current limits of microscopic resolution, radiation damage is a significant factor even for the radiation-resistant materials such as semiconductors and alloys.

In the historical development of electron-diffraction techniques the progress has depended to an important extent on the level of understanding of the dynamical diffraction processes and this understanding has followed, to a considerable degree, from the availability of electron microscopes. For the first 20 years of the development, with few exceptions, the lack of a precise knowledge of the specimen morphology meant that diffraction intensities were influenced to an unpredictable degree by dynamical scattering and the impression grew that electron-diffraction intensities could not meaningfully be interpreted.

It was the group in the Soviet Union, led initially by Dr Z. G. Pinsker and later by Dr B. K. Vainshtein and others, which showed that patterns from thin layers of a powder of microcrystals could be interpreted reliably by use of the kinematical approximation. The averaging over crystal orientation reduced the dynamical diffraction effects to the extent that practical structure analysis was feasible. The development of the techniques of using films of crystallites having strongly preferred orientations, to give patterns somewhat analogous to the X-ray rotation patterns, provided the basis for the collection of three-dimensional diffraction data on which many structure analyses have been based [see Section 2.5.4[link] and IT C (2004[link]), Section 4.3.5[link] ].

In recent years improvements in the techniques of specimen preparation and in the knowledge of the conditions under which dynamical diffraction effects become significant have allowed progress to be made with the use of high-energy electron diffraction patterns from thin single crystals for crystal structure analysis. Particularly for crystals of light-atom materials, including biological and organic compounds, the methods of structure analysis developed for X-ray diffraction, including the direct methods (see Section 2.5.7[link]), have been successfully applied in an increasing number of cases. Often it is possible to deduce some structural information from high-resolution electron-microscope images and this information may be combined with that from the diffraction intensities to assist the structure analysis process [see IT C (2004[link]), Section 4.3.8.8[link] ].

The determination of crystal symmetry by use of CBED (Section 2.5.3[link]) and the accurate determination of structure amplitudes by use of methods depending on the observation of dynamical diffraction effects [IT C (2004[link]), Section 4.3.7[link] ] came later, after the information on morphologies of crystals, and the precision electron optics associated with electron microscopes, became available.

In spite of the problem of radiation damage, a great deal of progress has been made in the study of organic and biological materials by electron-scattering methods. In some respects these materials are very favourable because, with only light atoms present, the scattering from thin films can be treated using the kinematical approximation without serious error. Because of the problem of radiation damage, however, special techniques have been evolved to maximize the information on the required structural aspects with minimum irradiation of the specimen. Image-processing techniques have been evolved to take advantage of the redundancy of information from a periodic structure and the means have been devised for combining information from multiple images and diffraction data to reconstruct specimen structure in three dimensions. These techniques are outlined in Sections 2.5.5[link] and 2.5.6[link]. They are based essentially on the application of the kinematical approximation and have been used very effectively within that limitation.

For most inorganic materials the complications of many-beam dynamical diffraction processes prevent the direct application of these techniques of image analysis, which depend on having a linear relationship between the image intensity and the value of the projected potential distribution of the sample. The smaller sensitivities to radiation damage can, to some extent, remove the need for the application of such methods by allowing direct visualization of structure with ultra-high-resolution images and the use of microdiffraction techniques.

2.5.2. Electron diffraction and electron microscopy1

| top | pdf |
J. M. Cowleya

2.5.2.1. Introduction

| top | pdf |

The contributions of electron scattering to the study of the structures of crystalline solids are many and diverse. This section will deal only with the scattering of high-energy electrons (in the energy range of [10^{4}] to [10^{6}] eV) in transmission through thin samples of crystalline solids and the derivation of information on crystal structures from diffraction patterns and high-resolution images. The range of wavelengths considered is from about 0.122 Å (12.2 pm) for 10 kV electrons to 0.0087 Å (0.87 pm) for 1 MeV electrons. Given that the scattering amplitudes of atoms for electrons have much the same form and variation with [(\sin \theta)/\lambda] as for X-rays, it is apparent that the angular range for strong scattering of electrons will be of the order of [10^{-2}] rad. Only under special circumstances, usually involving multiple elastic and inelastic scattering from very thick specimens, are scattering angles of more than [10^{-1}] rad of importance.

The strength of the interaction of electrons with matter is greater than that of X-rays by two or three orders of magnitude. The single-scattering, first Born approximation fails significantly for scattering from single heavy atoms. Diffracted beams from single crystals may attain intensities comparable with that of the incident beam for crystal thicknesses of [10^{2}] Å, rather than [10^{4}] Å or more. It follows that electrons may be used for the study of very thin samples, and that dynamical scattering effects, or the coherent interaction of multiply scattered electron waves, will modify the diffracted amplitudes in a significant way for all but very thin specimens containing only light atoms.

The experimental techniques for electron scattering are largely determined by the possibility of focusing electron beams by use of strong axial magnetic fields, which act as electron lenses having focal lengths as short as 1 mm or less. Electron microscopes employing such lenses have been produced with resolutions approaching 1 Å. With such instruments, images showing individual isolated atoms of moderately high atomic number may be obtained. The resolution available is sufficient to distinguish neighbouring rows of adjacent atoms in the projected structures of thin crystals viewed in favourable orientations. It is therefore possible in many cases to obtain information on the structure of crystals and of crystal defects by direct inspection of electron micrographs.

The electromagnetic electron lenses may also be used to form electron beams of very small diameter and very high intensity. In particular, by the use of cold field-emission electron guns, it is possible to obtain a current of [10^{-10}] A in an electron beam of diameter 10 Å or less with a beam divergence of less than [10^{-2}] rad, i.e. a current density of [10^{4}] A cm−2 or more. The magnitudes of the electron scattering amplitudes then imply that detectable signals may be obtained in diffraction from assemblies of fewer than [10^{2}] atoms. On the other hand, electron beams may readily be collimated to better than [10^{-6}] rad.

The cross sections for inelastic scattering processes are, in general, less than for the elastic scattering of electrons, but signals may be obtained by the observation of electron energy losses, or the production of secondary radiations, which allow the analysis of chemical compositions or electronic excited states for regions of the crystal 100 Å or less in diameter.

On the other hand, the transfer to the sample of large amounts of energy through inelastic scattering processes produces radiation damage which may severely limit the applicability of the imaging and diffraction techniques, especially for biological and organic materials, unless the information is gathered from large specimen volumes with low incident electron beam densities.

Structure analysis of crystals can be performed using electron diffraction in the same way as with X-ray or neutron diffraction. The mathematical expressions and the procedures are much the same. However, there are peculiarities of the electron-diffraction case which should be noted.

  • (1) Structure analysis based on electron diffraction is possible for thin specimens for which the conditions for kinematical scattering are approached, e.g. for thin mosaic single-crystal specimens, for thin polycrystalline films having a preferred orientation of very small crystallites or for very extensive, very thin single crystals of biological molecules such as membranes one or a few molecules thick.

  • (2) Dynamical diffraction effects are used explicitly in the determination of crystal symmetry (with no Friedel's law limitations) and for the measurement of structure amplitudes with high accuracy.

  • (3) For many radiation-resistant materials, the structures of crystals and of some molecules may be determined directly by imaging atom positions in projections of the crystal with a resolution of 2 Å or better. The information on atom positions is not dependent on the periodicity of the crystal and so it is equally possible to determine the structures of individual crystal defects in favourable cases.

  • (4) Techniques of microanalysis may be applied to the determination of the chemical composition of regions of diameter 100 Å or less using the same instrument as for diffraction, so that the chemical information may be correlated directly with morphological and structural information.

  • (5) Crystal-structure information may be derived from regions containing as few as [10^{2}] or [10^{3}] atoms, including very small crystals and single or multiple layers of atoms on surfaces.

2.5.2.2. The interactions of electrons with matter

| top | pdf |

  • (1) The elastic scattering of electrons results from the interaction of the charged electrons with the electrostatic potential distribution, [\varphi ({\bf r})], of the atoms or crystals. An incident electron of kinetic energy eW gains energy [e\varphi ({\bf r})] in the potential field. Alternatively it may be stated that an incident electron wave of wavelength [\lambda = h / mv] is diffracted by a region of variable refractive index [n ({\bf r}) = k / K_{0} = \{[W + \varphi ({\bf r})] / W\}^{1/2} \simeq 1 + \varphi ({\bf r}) / 2 W.]

  • (2) The most important inelastic scattering processes are:

    • (a) thermal diffuse scattering, with energy losses of the order of [2 \times 10^{-2}] eV, separable from the elastic scattering only with specially devised equipment; the angular distribution of thermal diffuse scattering shows variations with [(\sin \theta) / \lambda] which are much the same as for the X-ray case in the kinematical limit;

    • (b) bulk plasmon excitation, or the excitation of collective energy states of the conduction electrons, giving energy losses of 3 to 30 eV and an angular range of scattering of [10^{-4}] to [10^{-3}] rad;

    • (c) surface plasmons, or the excitation of collective energy states of the conduction electrons at discontinuities of the structure, with energy losses less than those for bulk plasmons and a similar angular range of scattering;

    • (d) interband or intraband excitation of valence-shell electrons giving energy losses in the range of 1 to [10^{2}] eV and an angular range of scattering of [10^{-4}] to [10^{-2}] rad;

    • (e) inner-shell excitations, with energy losses of [10^{2}] eV or more and an angular range of scattering of [10^{-3}] to [10^{-2}] rad, depending on the energy losses involved.

    • (3) In the original treatment by Bethe (1928)[link] of the elastic scattering of electrons by crystals, the Schrödinger equation is written for electrons in the periodic potential of the crystal; i.e. [\nabla^{2} \psi ({\bf r}) + K^{2}_0 [1 + \varphi ({\bf r}) / W] \psi ({\bf r}) = 0, \eqno (2.5.2.1)] where [\eqalignno{\varphi ({\bf r}) &= \textstyle\int V ({\bf u}) \exp \{-2 \pi i{\bf u} \cdot {\bf r}\} \;\hbox{d}{\bf u}\cr &= \textstyle\sum\limits_{\bf h} V_{\bf h} \exp \{-2 \pi i{\bf h} \cdot {\bf r}\}, &(2.5.2.2)}] K 0 is the wavevector in zero potential (outside the crystal) (magnitude [2\pi / \lambda]) and W is the accelerating voltage. The solutions of the equation are Bloch waves of the form [\psi ({\bf r}) = \textstyle\sum\limits_{\bf h} C_{\bf h} ({\bf k}) \exp \{-i ({\bf k}_{0} + 2 \pi {\bf h}) \cdot {\bf r}\}, \eqno (2.5.2.3)] where [{\bf k}_{0}] is the incident wavevector in the crystal and h is a reciprocal-lattice vector. Substitution of (2.5.2.2)[link] and (2.5.2.3)[link] in (2.5.2.1)[link] gives the dispersion equations [(\kappa^{2} - k_{\bf h}^{2}) C_{\bf h} + \textstyle\sum\limits_{\bf g}^{}{}^{\prime}\;V_{\bf h-g} C_{\bf g} = 0. \eqno (2.5.2.4)] Here κ is the magnitude of the wavevector in a medium of constant potential [V_{0}] (the `inner potential' of the crystal). The refractive index of the electron in the average crystal potential is then [n = \kappa / K = (1 + V_{0} / W)^{1/2} \simeq 1 + V_{0} / 2 W. \eqno (2.5.2.5)] Since [V_{0}] is positive and of the order of 10 V and W is [10^{4}] to [10^{6}] V, [n - 1] is positive and of the order of [10^{-4}].

      Solution of equation (2.5.2.4)[link] gives the Fourier coefficients [C_{\bf h}^{(i)}] of the Bloch waves [\psi^{(i)} ({\bf r})] and application of the boundary conditions gives the amplitudes of individual Bloch waves (see Chapter 5.2[link] ).

    • (4) The experimentally important case of transmission of high-energy electrons through thin specimens is treated on the assumption of a plane wave incident in a direction almost perpendicular to an infinitely extended plane-parallel lamellar crystal, making use of the small-angle scattering approximation in which the forward-scattered wave is represented in the paraboloidal approximation to the sphere. The incident-beam direction, assumed to be almost parallel to the z axis, is unique and the z component of k is factored out to give [\nabla^{2} \psi + 2k \sigma \varphi \psi = \pm i2k {\partial \psi \over \partial z}, \eqno (2.5.2.6)] where [k = 2\pi / \lambda] and [\sigma = 2\pi me\lambda / h^{2}]. [See Lynch & Moodie (1972)[link], Portier & Gratias (1981)[link], Tournarie (1962)[link], and Chapter 5.2[link] .]

      This equation is analogous to the time-dependent Schrödinger equation with z replacing t. Retention of the ± signs on the right-hand side is consistent with both ψ and [\psi^{*}] being solutions, corresponding to propagation in opposite directions with respect to the z axis. The double-valued solution is of importance in consideration of reciprocity relationships which provide the basis for the description of some dynamical diffraction symmetries. (See Section 2.5.3[link].)

    • (5) The integral form of the wave equation, commonly used for scattering problems, is written, for electron scattering, as [{\psi ({\bf r}) = \psi^{(0)} ({\bf r}) + (\sigma / \lambda) \int {\exp \{- i{\bf k}| {\bf r - r}'|\} \over |{\bf r - r}'|} \varphi ({\bf r}') \psi ({\bf r}') \;\hbox{d}{\bf r}'}. \eqno (2.5.2.7)]

      The wavefunction [\psi ({\bf r})] within the integral is approximated by using successive terms of a Born series [\psi ({\bf r}) = \psi^{(0)} ({\bf r}) + \psi^{(1)} ({\bf r}) + \psi^{(2)} ({\bf r}) + \ldots .\eqno (2.5.2.8)]

      The first Born approximation is obtained by putting [\psi ({\bf r}) = \psi^{(0)} ({\bf r})] in the integral and subsequent terms [\psi^{(n)} ({\bf r})] are generated by putting [\psi^{(n - 1)} ({\bf r})] in the integral.

      For an incident plane wave, [\psi^{(0)} ({\bf r}) = \exp \{- i{\bf k}_{0} \cdot {\bf r}\}] and for a point of observation at a large distance [{\bf R} = {\bf r} - {\bf r}'] from the scattering object [(|{\bf R}| \gg |{\bf r}'|)], the first Born approximation is generated as [\psi^{(1)} ({\bf r}) = {i\sigma \over \lambda R} \exp \{- i{\bf k} \cdot {\bf R}\} \int \varphi ({\bf r}') \exp \{i{\bf q} \cdot {\bf r}'\} \;\hbox{d}{\bf r}',] where [{\bf q} = {\bf k} - {\bf k}_{0}] or, putting [{\bf u} = {\bf q}/2\pi] and collecting the pre-integral terms into a parameter μ, [\Psi ({\bf u}) = \mu \textstyle\int \varphi ({\bf r}) \exp \{2\pi i{\bf u} \cdot {\bf r}\} \;\hbox{d}{\bf r}. \eqno (2.5.2.9)] This is the Fourier-transform expression which is the basis for the kinematical scattering approximation . It is derived on the basis that all [\psi^{(n)} ({\bf r})] terms for [n \neq 0] are very much smaller than [\psi^{(0)} ({\bf r})] and so is a weak scattering approximation.

      In this approximation, the scattered amplitude for an atom is related to the atomic structure amplitude, [f({\bf u})], by the relationship, derived from (2.5.2.8)[link], [\eqalignno{\psi ({\bf r}) &= \exp \{- i{\bf k}_{0} \cdot {\bf r}\} + i {\exp \{- i{\bf k} \cdot {\bf r}\} \over R\lambda} \sigma f({\bf u}),&\cr f({\bf u}) &= \textstyle\int \varphi ({\bf r}) \exp \{2\pi i{\bf u} \cdot {\bf r}\} \;\hbox{d}{\bf r}.&(2.5.2.10)\cr}] For centrosymmetrical atom potential distributions, the [f({\bf u})] are real, positive and monotonically decreasing with [|{\bf u}|]. A measure of the extent of the validity of the first Born approximation is given by the fact that the effect of adding the higher-order terms of the Born series may be represented by replacing [f({\bf u})] in (2.5.2.10)[link] by the complex quantities [f({\bf u}) = |{\bf f}| \exp \{i\eta ({\bf u})\}] and for single heavy atoms the phase factor η may vary from 0.2 for [|{\bf u}| = 0] to 4 or 5 for large [|{\bf u}|], as seen from the tables of IT C (2004[link], Section 4.3.3[link] ).

    • (6) Relativistic effects produce appreciable variations of the parameters used above for the range of electron energies considered. The relativistic values are [\eqalignno{m &= m_{0} (1 - v^{2} / c^{2})^{-1/2} = m_{0} (1 - \beta^{2})^{-1/2}, &(2.5.2.11)\cr \lambda &= h[2m_{0}|e| W (1 + |e| W / 2m_{0}c^{2})]^{-1/2} &(2.5.2.12)\cr &= \lambda_{c} (1 - \beta^{2})^{1/2} / \beta, &(2.5.2.13)}%(2.5.2.13)] where [\lambda_{c}] is the Compton wavelength, [\lambda_{c} = h / m_{0}c = 0.0242\,\hbox{\AA}], and [\eqalignno{\sigma &= 2\pi me\lambda / h^{2} = (2\pi m_{0}e / h^{2})(\lambda_{c} / \beta)\cr &= 2\pi / \{\lambda W[1 + (1 - \beta^{2})^{1/2}]\}. &(2.5.2.14)}] Values for these quantities are listed in IT C (2004[link], Section 4.3.2[link] ). The variations of λ and σ with accelerating voltage are illustrated in Fig. 2.5.2.1[link]. For high voltages, σ tends to a constant value, [2\pi m_{0}e\lambda_{c} / h^{2} = e / \hbar c].

      [Figure 2.5.2.1]

      Figure 2.5.2.1 | top | pdf |

      The variation with accelerating voltage of electrons of (a) the wavelength, λ and (b) the quantity [\lambda [1 + (h^{2} / m_{0}^{2} c^{2} \lambda^{2})] = \lambda_{c} / \beta] which is proportional to the interaction constant σ [equation (2.5.2.14)[link]]. The limit is the Compton wavelength [\lambda_{c}] (after Fujiwara, 1961[link]).

2.5.2.3. Recommended sign conventions

| top | pdf |

There are two alternative sets of signs for the functions describing wave optics. Both sets have been widely used in the literature. There is, however, a requirement for internal consistency within a particular analysis, independently of which set is adopted. Unfortunately, this requirement has not always been met and, in fact, it is only too easy at the outset of an analysis to make errors in this way. This problem might have come into prominence somewhat earlier were it not for the fact that, for centrosymmetric crystals (or indeed for centrosymmetric projections in the case of planar diffraction), only the signs used in the transmission and propagation functions can affect the results. It is not until the origin is set away from a centre of symmetry that there is a need to be consistent in every sign used.

Signs for electron diffraction have been chosen from two points of view: (1) defining as positive the sign of the exponent in the structure-factor expression and (2) defining the forward propagating free-space wavefunction with a positive exponent.

The second of these alternatives is the one which has been adopted in most solid-state and quantum-mechanical texts.

The first, or standard crystallographic convention, is the one which could most easily be adopted by crystallographers accustomed to retaining a positive exponent in the structure-factor equation. This also represents a consistent International Tables usage. It is, however, realized that both conventions will continue to be used in crystallographic computations, and that there are by now a large number of operational programs in use.

It is therefore recommended (a) that a particular sign usage be indicated as either standard crystallographic or alternative crystallographic to accord with Table 2.5.2.1[link], whenever there is a need for this to be explicit in publication, and (b) that either one or other of these systems be adhered to throughout an analysis in a self-consistent way, even in those cases where, as indicated above, some of the signs appear to have no effect on one particular conclusion.

Table 2.5.2.1| top | pdf |
Standard crystallographic and alternative crystallographic sign conventions for electron diffraction

 StandardAlternative
Free-space wave [\exp [- i({\bf k} \cdot {\bf r} - \omega t)]] [\exp [+ i({\bf k} \cdot {\bf r} - \omega t)]]
Fourier transforming from real space to reciprocal space [\textstyle\int \psi ({\bf r}) \exp [+ 2\pi i({\bf u} \cdot {\bf r})]\;\hbox{d}{\bf r}] [\textstyle\int \psi ({\bf r}) \exp [- 2\pi i({\bf u} \cdot {\bf r})]\;\hbox{d}{\bf r}]
Fourier transforming from reciprocal space to real space [\psi ({\bf r}) = \textstyle\int \Psi ({\bf u}) \exp [- 2\pi i({\bf u} \cdot {\bf r})]\;\hbox{d}{\bf u}] [\textstyle\int \Psi ({\bf u}) \exp [+ 2\pi i({\bf u} \cdot {\bf r})]\;\hbox{d}{\bf u}]
Structure factors [V({\bf h}) = (1/\Omega) \textstyle\sum_{j} f_{j} ({\bf h}) \exp (+ 2\pi i{\bf h} \cdot {\bf r}_{j})] [(1/\Omega) \textstyle\sum_{j} f_{j} ({\bf h}) \exp (- 2\pi i{\bf h} \cdot {\bf r}_{j})]
Transmission function (real space) [\exp [- i\sigma \varphi (x, y) \Delta z]] [\exp [+ i\sigma \varphi (x, y) \Delta z]]
Phenomenological absorption [\sigma \varphi ({\bf r}) - i\mu ({\bf r})] [\sigma \varphi ({\bf r}) + i\mu ({\bf r})]
Propagation function P(h) (reciprocal space) within the crystal [\exp (- 2\pi i\zeta_{\bf h} \Delta z)] [\exp (+ 2\pi i\zeta_{\bf h} \Delta z)]
Iteration (reciprocal space) [\Psi_{n + 1} ({\bf h}) = [\Psi_{n} ({\bf h}) \cdot P({\bf h})] \ast Q({\bf h})]  
Unitarity test (for no absorption) [T({\bf h}) = Q({\bf h}) \ast Q^{*} (- {\bf h}) = \delta ({\bf h})]  
Propagation to the image plane-wave aberration function, where [\chi (U) = \pi \lambda \Delta fU^{2} + \textstyle{1 \over 2} \pi C_{s} \lambda^{3} U^{4}], [U^{2} = u^{2} + v^{2}] and [\Delta f] is positive for overfocus [\exp [i\chi (U)]] [\exp [- i\chi (U)]]

[\sigma =] electron interaction constant [= 2\pi me\lambda/h^{2}]; [m =] (relativistic) electron mass; [\lambda =] electron wavelength; [e =] (magnitude of) electron charge; [h =] Planck's constant; [k = 2\pi/\lambda]; [\Omega =] volume of the unit cell; [{\bf u} =] continuous reciprocal-space vector, components u, v; [{\bf h} =] discrete reciprocal-space coordinate; [\varphi (x, y) =] crystal potential averaged along beam direction (positive); [\Delta z =] slice thickness; [\mu ({\bf r}) =] absorption potential [positive; typically [\leq 0.1 \sigma \varphi ({\bf r})]]; [\Delta f =] defocus (defined as negative for underfocus); [C_{s} =] spherical aberration coefficient; [\zeta_{\bf h} =] excitation error relative to the incident-beam direction and defined as negative when the point h lies outside the Ewald sphere; [f_{j} ({\bf h}) =] atomic scattering factor for electrons, [f_{e}], related to the atomic scattering factor for X-rays, [f_{X}], by the Mott formula [f_{e} = (e/\pi U^{2}) (Z - f_{X})]. [Q({\bf h})=] Fourier transform of periodic slice transmission function.

2.5.2.4. Scattering of electrons by crystals; approximations

| top | pdf |

The forward-scattering approximation to the many-beam dynamical diffraction theory outlined in Chapter 5.2[link] provides the basis for the calculation of diffraction intensities and electron-microscope image contrast for thin crystals. [See Cowley (1995)[link], Chapter 5.2[link] and IT C (2004[link]) Sections 4.3.6[link] and 4.3.8[link] .] On the other hand, there are various approximations which provide relatively simple analytical expressions, are useful for the determination of diffraction geometry, and allow estimates to be made of the relative intensities in diffraction patterns and electron micrographs in favourable cases.

  • (a) The kinematical approximation , derived in Section 2.5.2.2[link] from the first Born approximation, is analagous to the corresponding approximation of X-ray diffraction. It assumes that the scattering amplitudes are directly proportional to the three-dimensional Fourier transform of the potential distribution, [\varphi ({\bf r})]. [V({\bf u}) = \textstyle\int \varphi ({\bf r}) \exp \{2\pi i{\bf u} \cdot {\bf r}\} \;\hbox{d}{\bf r}, \eqno (2.5.2.15)] so that the potential distribution [\varphi ({\bf r})] takes the place of the charge-density distribution, [\rho ({\bf r})], relevant for X-ray scattering.

    The validity of the kinematical approximation as a basis for structure analysis is severely limited. For light-atom materials, such as organic compounds, it has been shown by Jap & Glaeser (1980)[link] that the thickness for which the approximation gives reasonable accuracy for zone-axis patterns from single crystals is of the order of 100 Å for 100 keV electrons and increases, approximately as [\sigma^{-1}], for higher energies. The thickness limits quoted for polycrystalline samples, having crystallite dimensions smaller than the sample thickness, are usually greater (Vainshtein, 1956[link]). For heavy-atom materials the approximation is more limited since it may fail significantly for single heavy atoms.

  • (b) The phase-object approximation (POA), or high-voltage limit, is derived from the general many-beam dynamical diffraction expression, equation (5.2.13.1), Chapter 5.2[link] , by assuming the Ewald sphere curvature to approach zero. Then the scattering by a thin sample can be expressed by multiplying the incoming wave amplitude by the transmission function [q(xy) = \exp \{-i\sigma \varphi (xy)\}, \eqno (2.5.2.16)] where [\varphi (xy) = \int \varphi ({\bf r})\;\hbox{d}z] is the projection of the potential distribution of the sample in the z direction, the direction of the incident beam. The diffraction-pattern amplitudes are then given by two-dimensional Fourier transform of (2.5.2.16)[link].

    This approximation is of particular value in relation to the electron microscopy of thin crystals. The thickness for its validity for 100 keV electrons is within the range 10 to 50 Å , depending on the accuracy and spatial resolution involved, and increases with accelerating voltage approximately as [\lambda^{-1/2}]. In computational work, it provides the starting point for the multi-slice method of dynamical diffraction calculations (IT C, 2004[link], Section 4.3.6.1[link] ).

  • (c) The two-beam approximation for dynamical diffraction of electrons assumes that only two beams, the incident beam and one diffracted beam (or two Bloch waves, each with two component amplitudes), exist in the crystal. This approximation has been adapted, notably by Hirsch et al. (1965)[link], for use in the electron microscopy of inorganic materials.

    It forms a convenient basis for the study of defects in crystals having small unit cells (metals, semiconductors etc.) and provides good preliminary estimates for the determination of crystal thicknesses and structure amplitudes for orientations well removed from principal axes, and for electron energies up to 200–500 keV, but it has decreasing validity, even for favourable cases, for higher energies. It has been used in the past as an `extinction correction' for powder-pattern intensities (Vainshtein, 1956[link]).

  • (d) The Bethe second approximation , proposed by Bethe (1928)[link] as a means for correcting the two-beam approximation for the effects of weakly excited beams, replaces the Fourier coefficients of potential by the `Bethe potentials' [U_{\bf h} = V_{\bf h} - 2 k_{0}\sigma \sum\limits_{\bf g} {V_{\bf g} \cdot V_{{\bf h} - {\bf g}}\over \kappa^{2} - k_{\bf g}^{2}}. \eqno (2.5.2.17)] Use of these potentials has been shown to account well for the deviations of powder-pattern intensities from the predictions of two-beam theory (Horstmann & Meyer, 1965[link]) and to predict accurately the extinctions of Kikuchi lines at particular accelerating voltages due to relativistic effects (Watanabe et al., 1968[link]), but they give incorrect results for the small-thickness limit.

2.5.2.5. Kinematical diffraction formulae

| top | pdf |

  • (1) Comparison with X-ray diffraction. The relations of real-space and reciprocal-space functions are analogous to those for X-ray diffraction [see equations (2.5.2.2)[link], (2.5.2.10)[link] and (2.5.2.15)[link]]. For diffraction by crystals [\eqalignno{\varphi ({\bf r}) &= \sum\limits_{\bf h} V_{\bf h} \exp \{-2 \pi i{\bf h \cdot r}\},\cr V_{\bf h} &= \int \varphi ({\bf r}) \exp \{2 \varphi i{\bf h \cdot r}\}\;\hbox{d} {\bf r} &(2.5.2.18)\cr &= {1\over \Omega} \sum\limits_{i} f_{i} ({\bf h}) \exp \{2 \pi i{\bf h \cdot r}_{i}\}, &(2.5.2.19)}%(2.5.2.19)] where the integral of (2.5.2.18)[link] and the summation of (2.5.2.19)[link] are taken over one unit cell of volume (see Dawson et al., 1974[link]).

    Important differences from the X-ray case arise because

    • (a) the wavelength is relatively small so that the Ewald-sphere curvature is small in the reciprocal-space region of appreciable scattering amplitude;

    • (b) the dimensions of the single-crystal regions giving appreciable scattering amplitudes are small so that the `shape transform' regions of scattering power around the reciprocal-lattice points are relatively large;

    • (c) the spread of wavelengths is small ([10^{-5}] or less, with no white-radiation background) and the degree of collimation is better ([10^{-4}] to [10^{-6}]) than for conventional X-ray sources.

      As a consequence of these factors, single-crystal diffraction patterns may show many simultaneous reflections, representing almost-planar sections of reciprocal space, and may show fine structure or intensity variations reflecting the crystal dimensions and shape.

  • (2) Kinematical diffraction-pattern intensities are calculated in a manner analogous to that for X-rays except that

    • (a) no polarization factor is included because of the small-angle scattering conditions;

    • (b) integration over regions of scattering power around reciprocal-lattice points cannot be assumed unless appropriate experimental conditions are ensured.

      For a thin, flat, lamellar crystal of thickness H, the observed intensity is [I_{\bf h}/I_{{\bf 0}} = |\sigma (V_{\bf h}/\Omega) (\sin \pi \zeta_{\bf h} H)/(\pi \zeta_{\bf h})|^{2}, \eqno (2.5.2.20)] where [\zeta_{\bf h}] is the excitation error for the h reflection and Ω is the unit-cell volume.

      For a single-crystal diffraction pattern obtained by rotating a crystal or from a uniformly bent crystal or for a mosaic crystal with a uniform distribution of orientations, the intensity is [I_{\bf h} = I_{{\bf 0}} {\sigma^{2} |V_{\bf h}|^{2} V_{c} d_{\bf h} \over 4\pi^{2} \Omega^{2}}, \eqno (2.5.2.21)] where [V_{c}] is the crystal volume and [d_{\bf h}] is the lattice-plane spacing. For a polycrystalline sample of randomly oriented small crystals, the intensity per unit length of the diffraction ring is [I_{\bf h} = I_{{\bf 0}} {\sigma^{2} |V_{\bf h}|^{2} V_{c} d_{\bf h}^{2} M_{\bf h} \over 8\pi^{2} \Omega^{2} L\lambda}, \eqno (2.5.2.22)] where [M_{\bf h}] is the multiplicity factor for the h reflection and L is the camera length, or the distance from the specimen to the detector plane. The special cases of `oblique texture' patterns from powder patterns having preferred orientations are treated in IT C (2004[link], Section 4.3.5[link] ).

    • (3) Two-beam dynamical diffraction formulae: complex potentials including absorption. In the two-beam dynamical diffraction approximation, the intensities of the directly transmitted and diffracted beams for transmission through a crystal of thickness H, in the absence of absorption, are [\eqalignno{I_{\bf0} &= (1 + w^{2})^{-1} \left[w^{2} + \cos^{2} \left\{{\pi H (1 + w^{2})^{1/2} \over \xi_{\bf h}}\right\}\right] &(2.5.2.23)\cr I_{\bf h} &= (1 + w^{2})^{-1} \sin^{2} \left\{{\pi H (1 + w^{2})^{1/2} \over \xi_{\bf h}}\right\}, &(2.5.2.24)}%(2.5.2.24)] where [\xi_{\bf h}] is the extinction distance, [\xi_{\bf h} = (2\sigma |V_{\bf h}|)^{-1}], and [w = \xi_{\bf h} \zeta_{\bf h} = \Delta \theta / (2\sigma |V_{\bf h}| d_{\bf h}), \eqno (2.5.2.25)] where [\Delta \theta] is the deviation from the Bragg angle.

      For the case that [\zeta_{\bf h} = 0], with the incident beam at the Bragg angle, this reduces to the simple Pendellösung expression [I_{\bf h} = 1 - I_{\bf 0} = \sin^{2} \{2 \pi \sigma |V_{\bf h}|H\}. \eqno (2.5.2.26)]

      The effects on the elastic Bragg scattering amplitudes of the inelastic or diffuse scattering may be introduced by adding an out-of-phase component to the structure amplitudes, so that for a centrosymmetric crystal, [V_{\bf h}] becomes complex by addition of an imaginary component. Alternatively, an absorption function [\mu({\bf r})], having Fourier coefficients [\mu_{\bf h}], may be postulated so that [\sigma V_{\bf h}] is replaced by [\sigma V_{\bf h} + i \mu_{\bf h}]. The [\mu_{\bf h}] are known as phenomenological absorption coefficients and their validity in many-beam diffraction has been demonstrated by, for example, Rez (1978)[link].

      The magnitudes [\mu_{\bf h}] depend on the nature of the experiment and the extent to which the various inelastically or diffusely scattered electrons are included in the measurements being made. If measurements are made of purely elastic scattering intensities for Bragg reflections or of image intensity variations due to the interaction of the sharp Bragg reflections only, the main contributions to the absorption coefficients are as follows (Radi, 1970[link]):

      • (a) from plasmon and single-electron excitations, [\mu_{0}] is of the order of 0.1 V0 and [\mu_{\bf h}], for [{\bf h} \neq 0], is negligibly small;

      • (b) from thermal diffuse scattering; [\mu_{\bf h}] is of the order of 0.1 Vh and decreasing more slowly than [V_{\bf h}] with scattering angle.

        Including absorption effects in (2.5.2.26)[link] for the case [\zeta_{\bf h} = 0] gives [\eqalign{I_{\bf 0} &= {\textstyle{1 \over 2}} \exp \{ - \mu_{0}H\} [\cosh \mu_{\bf h}H + \cos (2 \pi \sigma V_{\bf h}H)],\cr I_{\bf h}&= {\textstyle{1 \over 2}} \exp \{ - \mu_{0}H\} [\cosh \mu_{\bf h}H - \cos (2 \pi \sigma V_{\bf h}H)].\cr} \eqno (2.5.2.27)] The Borrmann effect is not very pronounced for electrons because [\mu_{\bf h} \ll \mu_{\bf 0}], but can be important for the imaging of defects in thick crystals (Hirsch et al., 1965[link]; Hashimoto et al., 1961[link]).

        Attempts to obtain analytical solutions for the dynamical diffraction equations for more than two beams have met with few successes. There are some situations of high symmetry, with incident beams in exact zone-axis orientations, for which the many-beam solution can closely approach equivalent two- or three-beam behaviour (Fukuhara, 1966[link]). Explicit solutions for the three-beam case, which displays some aspects of many-beam character, have been obtained (Gjønnes & Høier, 1971[link]; Hurley & Moodie, 1980[link]).

2.5.2.6. Imaging with electrons

| top | pdf |

Electron optics. Electrons may be focused by use of axially symmetric magnetic fields produced by electromagnetic lenses. The focal length of such a lens used as a projector lens (focal points outside the lens field) is given by[f_{p}^{-1} = {e \over 8mW_{r}} \int\limits_{-\infty}^{\infty} H_{z}^{2}(z) \;\hbox{d}z, \eqno (2.5.2.28)] where [W_{r}] is the relativistically corrected accelerating voltage and [H_{z}] is the z component of the magnetic field. An expression in terms of experimental constants was given by Liebman (1955)[link] as [{1 \over f} = {A_{0}(NI)^{2} \over W_{r}(S + D)}, \eqno (2.5.2.29)] where [A_{0}] is a constant, NI is the number of ampere turns of the lens winding, S is the length of the gap between the magnet pole pieces and D is the bore of the pole pieces.

Lenses of this type have irreducible aberrations, the most important of which for the paraxial conditions of electron microscopy is the third-order spherical aberration, coefficient [C_{s}], giving a variation of focal length of [C_{s}\alpha^{2}] for a beam at an angle α to the axis. Chromatic aberration, coefficient [C_{c}], gives a spread of focal lengths [\Delta f = C_{c} \left({\Delta W_{0} \over W_{0}} + 2{\Delta I \over I}\right) \eqno (2.5.2.30)] for variations [\Delta W_{0}] and [\Delta I] of the accelerating voltage and lens currents, respectively.

The objective lens of an electron microscope is the critical lens for the determination of image resolution and contrast. The action of this lens in a conventional transmission electron microscope (TEM) is described by use of the Abbe theory for coherent incident illumination transmitted through the object to produce a wavefunction [\psi_{0} (xy)] (see Fig. 2.5.2.2[link]).

[Figure 2.5.2.2]

Figure 2.5.2.2 | top | pdf |

Diagram representing the critical components of a conventional transmission electron microscope (TEM) and a scanning transmission electron microscope (STEM). For the TEM, electrons from a source A illuminate the specimen and the objective lens forms an image of the transmitted electrons on the image plane, B. For the STEM, a source at B is imaged by the objective lens to form a small probe on the specimen and some part of the transmitted beam is collected by a detector at A.

The amplitude distribution in the back focal plane of the objective lens is written [\Psi_{0} (u, v) \cdot T (u, v), \eqno (2.5.2.31)] where [\Psi_{0} (u, v)] is the Fourier transform of [\psi_{0} (x, y)] and T(u, v) is the transfer function of the lens, consisting of an aperture function [A (u, v) = \cases{1 &${\rm for }\;\;(u^{2} + v^{2})^{1/2} \leq A$\cr 0 &elsewhere\cr} \eqno (2.5.2.32)] and a phase function exp [\{i\chi (u, v)\}] where the phase perturbation [\chi (uv)] due to lens defocus [\Delta f] and aberrations is usually approximated as [\chi (uv) = \pi \cdot \Delta f \cdot \lambda (u^{2} + v^{2}) + {\pi\over 2}C_{s} \lambda^{3} (u^{2} + v^{2})^{2}, \eqno (2.5.2.33)] and u, v are the reciprocal-space variables related to the scattering angles [\varphi_{x}], [\varphi_{y}] by [\eqalign{u &= (\sin \varphi_{x})/\lambda,\cr v &= (\sin \varphi_{y})/\lambda.\cr}]

The image amplitude distribution, referred to the object coordinates, is given by Fourier transform of (2.5.2.31)[link] as [\psi (xy) = \psi_{0} (xy) \ast t (xy), \eqno (2.5.2.34)] where [t (xy)], given by Fourier transform of [{T (u, v)}], is the spread function. The image intensity is then [I (xy) = |\psi (xy)|^{2} = |\psi_{0} (xy) \ast t (xy)|^{2}. \eqno (2.5.2.35)]

In practice the coherent imaging theory provides a good approximation but limitations of the coherence of the illumination have appreciable effects under high-resolution imaging conditions.

The variation of focal lengths according to (2.5.2.30)[link] is described by a function [G (\Delta f)]. Illumination from a finite incoherent source gives a distribution of incident-beam angles [H (u_{1}, v_{1})]. Then the image intensity is found by integrating incoherently over [\Delta f] and [u_{1}, v_{1}]:[ \eqalignno{I (xy) &= \textstyle\int \textstyle\int G (\Delta f) \cdot H (u_{1} v_{1})\cr &\quad\times |{\scr F} \{\Psi_{0} (u - u_{1}, v - v_{1}) \cdot T_{\Delta f} (u, v)\}|^{2} \;\hbox{d} (\Delta f) \cdot \;\hbox{d} u_{1} \;\hbox{d}v_{1},\cr& &(2.5.2.36)}] where [ {\scr F}] denotes the Fourier-transform operation.

In the scanning transmission electron microscope (STEM), the objective lens focuses a small bright source of electrons on the object and directly transmitted or scattered electrons are detected to form an image as the incident beam is scanned over the object (see Fig. 2.5.2.2[link]). Ideally the image amplitude can be related to that of the conventional transmission electron microscope by use of the `reciprocity relationship' which refers to point sources and detectors for scalar radiation in scalar fields with elastic scattering processes only. It may be stated: `The amplitude at a point B due to a point source at A is identical to that which would be produced at A for the identical source placed at B'.

For an axial point source, the amplitude distribution produced by the objective lens on the specimen is [ {\scr F} [T (u, v)] = t (xy). \eqno (2.5.2.37)] If this is translated by the scan to X, Y, the transmitted wave is [\psi_{0} (xy) = q (xy) \cdot t (x - X, y - Y). \eqno (2.5.2.38)]

The amplitude on the plane of observation following the specimen is then [\Psi (uv) = Q (u, v) \ast \{T (uv) \exp [2 \pi i(uX + vY)]\}, \eqno (2.5.2.39)] and the image signal produced by a detector having a sensitivity function H(u, v) is [\eqalignno{I (X, Y) &= \textstyle\int H (u, v) |Q (u, v) \ast T (u, v)&\cr &\quad\times \exp \{2 \pi i (uX + vY)\}|^{2} \;\hbox{d}u \;\hbox{d}v. &\cr &&(2.5.2.40)\cr}]If H(u, v) represents a small detector, approximated by a delta function, this becomes [I (x, y) = |q (xy) \ast t (xy)|^{2}, \eqno (2.5.2.41)] which is identical to the result (2.5.2.35)[link] for a plane incident wave in the conventional transmission electron microscope.

2.5.2.7. Imaging of very thin and weakly scattering objects

| top | pdf |

  • (a) The weak-phase-object approximation. For sufficiently thin objects, the effect of the object on the incident-beam amplitude may be represented by the transmission function (2.5.2.16)[link] given by the phase-object approximation. If the fluctuations, [\varphi (xy) - \bar{\varphi}], about the mean value of the projected potential are sufficiently small so that [\sigma [\varphi (xy) - \bar{\varphi}] \ll 1], it is possible to use the weak-phase-object approximation (WPOA) [q (xy) = \exp \{- i\sigma \varphi (xy)\} = 1 - i\sigma \varphi (xy), \eqno (2.5.2.42)] where [\varphi (xy)] is referred to the average value, [\bar{\varphi}]. The assumption that only first-order terms in [\sigma \varphi (xy)] need be considered is the equivalent of a single-scattering, or kinematical, approximation applied to the two-dimensional function, the projected potential of (2.5.2.16)[link]. From (2.5.2.42)[link], the image intensity (2.5.2.35)[link] becomes [I (xy) = 1 + 2\sigma \varphi (xy) \ast s (xy), \eqno (2.5.2.43)] where the spread function s(xy) is the Fourier transform of the imaginary part of T(uv), namely [A(uv)\sin\chi (uv)].

    The optimum imaging condition is then found, following Scherzer (1949)[link], by specifying that the defocus should be such that [|\sin \chi|] is close to unity for as large a range of [U = (u^{2} + v^{2})^{1/2}] as possible. This is so for a negative defocus such that [\chi (uv)] decreases to a minimum of about [-2 \pi /3] before increasing to zero and higher as a result of the fourth-order term of (2.5.2.33)[link] (see Fig. 2.5.2.3[link]). This optimum, `Scherzer defocus' value is given by [{\hbox{d}\chi \over \hbox{d}u} = 0 \quad \hbox{for} \quad \chi = -2 \pi /3] or [\Delta f = -\left(\textstyle{4 \over 3} C_{s} \lambda \right)^{1/2}. \eqno (2.5.2.44)]

    [Figure 2.5.2.3]

    Figure 2.5.2.3 | top | pdf |

    The functions [\chi (U)], the phase factor for the transfer function of a lens given by equation (2.5.2.33)[link], and [\sin \chi (U)] for the Scherzer optimum defocus condition, relevant for weak phase objects, for which the minimum value of [\chi (U)] is [-2\pi /3].

    The resolution limit is then taken as corresponding to the value of [U = 1.51 C_{s}^{-1/4} \lambda^{-3/4}] when [\sin\chi] becomes zero, before it begins to oscillate rapidly with U. The resolution limit is then [\Delta x = 0.66 C_{s}^{1/4} \lambda^{3/4}. \eqno (2.5.2.45)] For example, for [C_{s} = 1] mm and [\lambda = 2.51 \times 10^{-2}] Å (200 keV), [\Delta x = 2.34] Å.

    Within the limits of the WPOA, the image intensity can be written simply for a number of other imaging modes in terms of the Fourier transforms [c ({\bf r})] and [s ({\bf r})] of the real and imaginary parts of the objective-lens transfer function [T ({\bf u}) = A ({\bf u}) \exp \{i \chi ({\bf u})\}], where r and u are two-dimensional vectors in real and reciprocal space, respectively.

    For dark-field TEM images, obtained by introducing a central stop to block out the central beam in the diffraction pattern in the back-focal plane of the objective lens, [I ({\bf r}) = [\sigma \varphi ({\bf r}) \ast c ({\bf r})]^{2} + [\sigma \varphi ({\bf r}) \ast s ({\bf r})]^{2}. \eqno (2.5.2.46)] Here, as in (2.5.2.42)[link], [\varphi ({\bf r})] should be taken to imply the difference from the mean potential value, [\varphi ({\bf r}) - \bar{\varphi}].

    For bright-field STEM imaging with a very small detector placed axially in the central beam of the diffraction pattern (2.5.2.39)[link] on the detector plane, the intensity, from (2.5.2.41)[link], is given by (2.5.2.43)[link].

    For a finite axially symmetric detector, described by [D ({\bf u})], the image intensity is [{I ({\bf r}) = 1 + 2 \sigma \varphi ({\bf r}) \ast \{s ({\bf r}) [d ({\bf r}) * c ({\bf r})] - c ({\bf r}) [d ({\bf r}) \ast s ({\bf r})]\}}, \eqno (2.5.2.47)] where [d ({\bf r})] is the Fourier transform of [D ({\bf u})] (Cowley & Au, 1978[link]).

    For STEM with an annular dark-field detector which collects all electrons scattered outside the central spot of the diffraction pattern in the detector plane, it can be shown that, to a good approximation (valid except near the resolution limit) [I ({\bf r}) = \sigma^{2} \varphi^{2} ({\bf r}) \ast [c^{2} ({\bf r}) + s^{2} ({\bf r})]. \eqno (2.5.2.48)] Since [c^{2} ({\bf r}) + s^{2} ({\bf r}) = |t ({\bf r})|^{2}] is the intensity distribution of the electron probe incident on the specimen, (2.5.2.48)[link] is equivalent to the incoherent imaging of the function [\sigma^{2} \varphi^{2} ({\bf r})].

    Within the range of validity of the WPOA or, in general, whenever the zero beam of the diffraction pattern is very much stronger than any diffracted beam, the general expression (2.5.2.36)[link] for the modifications of image intensities due to limited coherence may be conveniently approximated. The effect of integrating over the variables [\Delta f, u_{1}, v_{1}], may be represented by multiplying the transfer function T (u, v) by so-called `envelope functions' which involve the Fourier transforms of the functions [G (\Delta f)] and [H (u_{1}, v_{1})].

    For example, if [G(\Delta f)] is approximated by a Gaussian of width [epsilon] (at [e^{-1}] of the maximum) centred at [\Delta f_{0}] and [H(u_{1} v_{1})] is a circular aperture function [H(u_{1} v_{1}) = \cases{1 &${\rm if}\;\;u_{1}, v_{1}\!\!\;\lt\!\; b$\cr 0 &otherwise,\cr}] the transfer function [T_{0}(uv)] for coherent radiation is multiplied by [\exp \{-\pi^{2} \lambda^{2} \varepsilon^{2} (u^{2} + v^{2})^{2}/4\} \cdot J_{1} (\pi B \eta)/(\pi B \eta)] where [\eqalignno{\eta &= f_{0}\lambda (u + v) +C_{s}\lambda^{3} (u^{3} + v^{3})&\cr &\quad + \pi i \varepsilon^{2} \lambda^{2} (u^{3} + u^{2} v + uv^{2} + v^{3})/2. &(2.5.2.49)}]

  • (b) The projected charge-density approximation. For very thin specimens composed of moderately heavy atoms, the WPOA is inadequate. Within the region of validity of the phase-object approximation (POA), more complicated relations analagous to (2.5.2.43)[link] to (2.5.2.47)[link] may be written. A simpler expression may be obtained by use of the two-dimensional form of Poisson's equation, relating the projected potential distribution [\varphi (xy)] to the projected charge-density distribution [\rho (xy)]. This is the PCDA (projected charge-density approximation) (Cowley & Moodie, 1960[link]), [I (xy) = 1 + 2\Delta f \cdot \lambda \sigma \rho (xy). \eqno (2.5.2.50)]

    This is valid for sufficiently small values of the defocus [\Delta f\!], provided that the effects of the spherical aberration may be neglected, i.e. for image resolutions not too close to the Scherzer resolution limit (Lynch et al., 1975[link]). The function [\rho (xy)] includes contributions from both the positive atomic nuclei and the negative electron clouds. For underfocus ([\Delta f] negative), single atoms give dark spots in the image. The contrast reverses with defocus.

2.5.2.8. Crystal structure imaging

| top | pdf |

  • (a) Introduction. It follows from (2.5.2.43)[link] and (2.5.2.42)[link] that, within the severe limitations of validity of the WPOA or the PCDA, images of very thin crystals, viewed with the incident beam parallel to a principal axis, will show dark spots at the positions of rows of atoms parallel to the incident beam. Provided that the resolution limit is less than the projected distances between atom rows (1–3 Å), the projection of the crystal structure may be seen directly.

    In practice, theoretical and experimental results suggest that images may give a direct, although non-linear, representation of the projected potential or charge-density distribution for thicknesses much greater than the thicknesses for validity of these approximations, e.g. for thicknesses which may be 50 to 100 Å for 100 keV electrons for 3 Å resolutions and which increase for comparable resolutions at higher voltage but decrease with improved resolutions.

    The use of high-resolution imaging as a means for determining the structures of crystals and for investigating the form of the defects in crystals in terms of the arrangement of the atoms has become a widely used and important branch of crystallography with applications in many areas of solid-state science. It must be emphasized, however, that image intensities are strongly dependent on the crystal thickness and orientation and also on the instrumental parameters (defocus, aberrations, alignment etc.). It is only when all of these parameters are correctly adjusted to lie within strictly defined limits that interpretation of images in terms of atom positions can be attempted [see IT C (2004[link], Section 4.3.8[link] )].

    Reliable interpretations of high-resolution images of crystals (`crystal structure images') may be made, under even the most favourable circumstances, only by the comparison of observed image intensities with intensities calculated by use of an adequate approximation to many-beam dynamical diffraction theory [see IT C (2004[link], Section 4.3.6[link] )]. Most calculations for moderate or large unit cells are currently made by the multi-slice method based on formulation of the dynamical diffraction theory due to Cowley & Moodie (1957)[link]. For smaller unit cells, the matrix method based on the Bethe (1928)[link] formulation is also frequently used (Hirsch et al., 1965[link]).

  • (b) Fourier images. For periodic objects in general, and crystals in particular, the amplitudes of the diffracted waves in the back focal plane are given from (2.5.2.31)[link] by [\Psi_{0} ({\bf h}) \cdot T ({\bf h}). \eqno (2.5.2.51)] For rectangular unit cells of the projected unit cell, the vector h has components [h/a] and [k/b]. Then the set of amplitudes (2.5.2.34)[link], and hence the image intensities, will be identical for two different sets of defocus and spherical aberration values [\Delta f_{1}, C_{s1}] and [\Delta f_{2}, C_{s2}] if, for an integer N, [\chi_{1} (h) = \chi_{2} (h) = 2 N\pi\hbox{;}] i.e. [{\pi \lambda \left({h^{2} \over a^{2}} + {k^{2} \over b^{2}}\right) (\Delta f_{1} - \Delta f_{2}) + {1 \over 2} \pi \lambda^{3} \left({h^{2} \over a^{2}} + {k^{2} \over b^{2}}\right)^{2} (C_{s1} - C_{s2}) = 2\pi N.}]

    This relationship is satisfied for all h, k if [a^{2}/b^{2}] is an integer and [\Delta f_{1} - \Delta f_{2} = 2na^{2}/\lambda] and [C_{s1} - C_{s2} = 4ma^{4}/\lambda^{3}, \eqno (2.5.2.52)] where m, n are integers (Kuwabara, 1978[link]). The relationship for [\Delta f] is an expression of the Fourier image phenomenon, namely that for a plane-wave incidence, the intensity distribution for the image of a periodic object repeats periodically with defocus (Cowley & Moodie, 1960[link]). Hence it is often necessary to define the defocus value by observation of a non-periodic component of the specimen such as a crystal edge (Spence et al., 1977[link]).

    For the special case [a^{2} = b^{2}], the image intensity is also reproduced exactly for [\Delta f_{1} - \Delta f_{2} = (2n + 1) a^{2}/\lambda, \eqno (2.5.2.53)] except that in this case the image is translated by a distance [a/2] parallel to each of the axes.

2.5.2.9. Image resolution

| top | pdf |

  • (1) Definition and measurement. The `resolution' of an electron microscope or, more correctly, the `least resolvable distance', is usually defined by reference to the transfer function for the coherent imaging of a weak phase object under the Scherzer optimum defocus condition (2.5.2.44)[link]. The resolution figure is taken as the inverse of the U value for which [\sin\chi (U)] first crosses the axis and is given, as in (2.5.2.45)[link], by [\Delta x = 0.66C_{s}^{1/4} \lambda^{3/4}. \eqno (2.5.2.45)]

    It is assumed that an objective aperture is used to eliminate the contribution to the image for U values greater than the first zero crossing, since for these contributions the relative phases are distorted by the rapid oscillations of [\sin \chi (U)] and the corresponding detail of the image is not directly interpretable in terms of the projection of the potential distribution of the object.

    The resolution of the microscope in practice may be limited by the incoherent factors which have the effect of multiplying the WPOA transfer function by envelope functions as in (2.5.2.49)[link].

    The resolution, as defined above, and the effects of the envelope functions may be determined by Fourier transform of the image of a suitable thin, weakly scattering amorphous specimen. The Fourier-transform operation may be carried out by use of an optical diffractometer. A more satisfactory practice is to digitize the image directly by use of a two-dimensional detector system in the microscope or from a photographic recording, and perform the Fourier transform numerically.

    For the optical diffractometer method, the intensity distribution obtained is given from (2.5.2.43)[link] as a radially symmetric function of U, [ \eqalignno{I (U) &= |{\scr F} I (xy)|^{2}&\cr &= \delta (U) + 4\sigma^{2} |\Phi (u)|^{2} \cdot \sin^{2} \chi (U) \cdot E^{2} (U), &(2.5.2.54)}] where [E(U)] is the product of the envelope functions.

    In deriving (2.5.2.54)[link] it has been assumed that:

    • (a) the WPOA applies;

    • (b) the optical transmission function of the photographic record is linearly related to the image intensity, [I(xy)];

    • (c) the diffraction intensity [|\Phi (U)|^{2}] is a radially symmetric, smoothly varying function such as is normally produced by a sufficiently large area of the image of an amorphous material;

    • (d) there is no astigmatism present and no drift of the specimen; either of these factors would remove the radial symmetry.

      From the form of (2.5.2.54)[link] and a preknowledge of [|\Phi (U)|^{2}], the zero crossings of [\sin\chi] and the form of [E(U)] may be deduced. Analysis of a through-focus series of images provides more complete and reliable information.

  • (2) Detail on a scale much smaller than the resolution of the electron microscope, as defined above, is commonly seen in electron micrographs, especially for crystalline samples. For example, lattice fringes, having the periodicity of the crystal lattice planes, with spacings as small as 0.6 Å in one direction, have been observed using a microscope having a resolution of about 2.5 Å (Matsuda et al., 1978[link]), and two-dimensionally periodic images showing detail on the scale of 0.5 to 1 Å have been observed with a similar microscope (Hashimoto et al., 1977[link]).

    Such observations are possible because

    • (a) for periodic objects the diffraction amplitude [\Psi_{0} (uv)] in (2.5.2.31)[link] is a set of delta functions which may be multiplied by the corresponding values of the transfer function that will allow strong interference effects between the diffracted beams and the zero beam, or between different diffracted beams;

    • (b) the envelope functions for the WPOA, arising from incoherent imaging effects, do not apply for strongly scattering crystals; the more general expression (2.5.2.36)[link] provides that the incoherent imaging factors will have much less effect on the interference of some sets of diffracted beams.

      The observation of finely spaced lattice fringes provides a measure of some important factors affecting the microscope performance, such as the presence of mechanical vibrations, electrical interference or thermal drift of the specimen. A measure of the fineness of the detail observable in this type of image may therefore be taken as a measure of `instrumental resolution'.

2.5.2.10. Electron diffraction in electron microscopes

| top | pdf |

Currently most electron-diffraction patterns are obtained in conjunction with images, in electron microscopes of one form or another, as follows.

  • (a) Selected-area electron-diffraction (SAED) patterns are obtained by using intermediate and projector lenses to form an image of the diffraction pattern in the back-focal plane of the objective lens (Fig. 2.5.2.2).[link] The area of the specimen from which the diffraction pattern is obtained is defined by inserting an aperture in the image plane of the objective lens. For parallel illumination of the specimen, sharp diffraction spots are produced by perfect crystals.

    A limitation to the area of the specimen from which the diffraction pattern can be obtained is imposed by the spherical aberration of the objective lens. For a diffracted beam scattered through an angle α, the spread of positions in the object for which the diffracted beam passes through a small axial aperture in the image plane is [C_{s} \alpha^{3}], e.g. for [C_{s} = 1] mm, [\alpha = 5 \times 10^{-2}] rad ( [10_{'}0_{'}0] reflection from gold for 100 keV electrons), [C_{s} \alpha^{3} = 1250] Å, so that a selected-area diameter of less than about 2000 Å is not feasible. For higher voltages, the minimum selected-area diameter decreases with [\lambda^{2}] if the usual assumption is made that [C_{s}] increases for higher-voltage microscopes so that [C_{s}\lambda] is a constant.

  • (b) Convergent-beam electron-diffraction (CBED) patterns are obtained when an incident convergent beam is focused on the specimen, as in an STEM instrument or an STEM attachment for a conventional TEM instrument.

    For a large, effectively incoherent source, such as a conventional hot-filament electron gun, the intensities are added for each incident-beam direction. The resulting CBED pattern has an intensity distribution[I (uv) = \textstyle\int |\Psi_{u_{1} v_{1}} (uv)|^{2} \;\hbox{d}u_{1} \;\hbox{d}v_{1}, \eqno (2.5.2.55)] where [\Psi_{u_{1} v_{1}} (uv)] is the Fourier transform of the exit wave at the specimen for an incident-beam direction [u_{1}, v_{1}].

  • (c) Coherent illumination from a small bright source such as a field emission gun may be focused on the specimen to give an electron probe having an intensity distribution [|t(xy)|^{2}] and a diameter equal to the STEM dark-field image resolution [equation (2.5.2.47)[link]] of a few Å. The intensity distribution of the resulting microdiffraction pattern is then [|\Psi (uv)|^{2} = |\Psi_{0}(uv) \ast T (uv)|^{2}, \eqno (2.5.2.56)] where [\Psi_{0}(uv)] is the Fourier transform of the exit wave at the specimen. Interference occurs between waves scattered from the various incident-beam directions. The diffraction pattern is thus an in-line hologram as envisaged by Gabor (1949)[link].

  • (d) Diffraction patterns may be obtained by using an optical diffractometer (or computer) to produce the Fourier transform squared of a small selected region of a recorded image. The optical diffraction-pattern intensity obtained under the ideal conditions specified under equation (2.5.2.54)[link] is given, in the case of weak phase objects, by [I (uv) = \delta (uv) + 4\sigma^{2}| \Phi (uv)|^{2} \cdot \sin^{2} \chi (uv) \cdot E^{2} (uv) \eqno (2.5.2.57)] or, more generally, by [I(uv) = c\delta (uv) + |\Psi (uv) \cdot T(uv) \ast \Psi^{*}(uv) \cdot T^{*}(uv)|^{2},] where [\Psi (uv)] is the Fourier transform of the wavefunction at the exit face of the specimen and c is a constant depending on the characteristics of the photographic recording medium.

2.5.3. Space-group determination by convergent-beam electron diffraction2

| top | pdf |
P. Goodmanb

2.5.3.1. Introduction

| top | pdf |

2.5.3.1.1. CBED

| top | pdf |

Convergent-beam electron diffraction, originating in the experiments of Kossel and Möllenstedt (Kossel & Möllenstedt, 1938[link]) has been established over the past two decades as a powerful technique for the determination of space group in inorganic materials, with particular application when only microscopic samples are available. Relatively recently, with the introduction of the analytical electron microscope, this technique – abbreviated as CBED – has become available as a routine, so that there is now a considerable accumulation of data from a wide range of materials. A significant extension of the technique in recent times has been the introduction of LACBED (large-angle CBED) by Tanaka & Terauchi (1985[link]). This technique allows an extensive angular range of single diffraction orders to be recorded and, although this method cannot be used for microdiffraction (since it requires an extensive single-crystal area), new LACBED applications appear regularly, particularly in the field of semiconductor research (see Section 2.5.3.6[link]).

The CBED method relies essentially on two basic properties of transmission electron diffraction, namely the radical departure from Friedel's law and the formation of characteristic extinction bands within space-group-forbidden reflections. Departure from Friedel's law in electron diffraction was first noted experimentally by Miyake & Uyeda (1950)[link]. The prediction of space-group-forbidden bands (within space-group-forbidden reflections) by Cowley & Moodie (1959)[link], on the other hand, was one of the first successes of N-beam theory. A detailed explanation was later given by Gjønnes & Moodie (1965)[link]. These are known variously as `GM' bands (Tanaka et al., 1983[link]), or more simply and definitively as `GS' (glide–screw) bands (this section). These extinctions have a close parallel with space-group extinctions in X-ray diffraction, with the reservation that only screw axes of order two are accurately extinctive under N-beam conditions. This arises from the property that only those operations which lead to identical projections of the asymmetric unit can have N-beam dynamical symmetries (Cowley et al., 1961[link]).

Additionally, CBED from perfect crystals produces high-order defect lines in the zero-order pattern, analogous to the defect Kikuchi lines of inelastic scattering, which provide a sensitive measurement of unit-cell parameters (Jones et al., 1977[link]; Fraser et al., 1985[link]; Tanaka & Terauchi, 1985[link]).

The significant differences between X-ray and electron diffraction, which may be exploited in analysis, arise as a consequence of a much stronger interaction in the case of electrons (Section 2.5.2[link]). Hence, thin, approximately parallel-sided crystal regions must be used in high-energy (100 kV–1 MV) electron transmission work, so that diffraction is produced from crystals effectively infinitely periodic in only two dimensions, leading to the relaxation of three-dimensional diffraction conditions known as `excitation error' (Chapter 5.2[link] ). Also, there is the ability in CBED to obtain data from microscopic crystal regions of around 50 Å in diameter, with corresponding exposure times of several seconds, allowing a survey of a material to be carried out in a relatively short time.

In contrast, single-crystal X-ray diffraction provides much more limited symmetry information in a direct fashion [although statistical analysis of intensities (Wilson, 1949[link]) will considerably supplement this information], but correspondingly gives much more direct three-dimensional geometric data, including the determination of unit-cell parameters and three-dimensional extinctions.

The relative strengths and weaknesses of the two techniques make it useful where possible to collect both convergent-beam and X-ray single-crystal data in a combined study. However, all parameters can be obtained from convergent-beam and electron-diffraction data, even if in a somewhat less direct form, making possible space-group determination from microscopic crystals and microscopic regions of polygranular material. Several reviews of the subject are available (Tanaka, 1994[link]; Steeds & Vincent, 1983[link]; Steeds, 1979[link]). In addition, an atlas of characteristic CBED patterns for direct phase identification of metal alloys has been published (Mansfield, 1984[link]), and it is likely that this type of procedure, allowing N-beam analysis by comparison with standard simulations, will be expanded in the near future.

2.5.3.1.2. Zone-axis patterns from CBED

| top | pdf |

Symmetry analysis is necessarily tied to examination of patterns near relevant zone axes, since the most intense N-beam interaction occurs amongst the zero-layer zone-axis reflections, with in addition a limited degree of upper-layer (higher-order Laue zone) interaction. There will generally be several useful zone axes accessible for a given parallel-sided single crystal, with the regions between axes being of little use for symmetry analysis. Only one such zone axis can be parallel to a crystal surface normal, and a microcrystal is usually chosen at least initially to have this as the principal symmetry axis. Other zone axes from that crystal may suffer mild symmetry degradation because the N-beam lattice component (`excitation error' extension) will not have the symmetry of the structure (Goodman, 1974[link]; Eades et al., 1983[link]).

Upper-layer interactions, responsible for imparting three-dimensional information to the zero layer, are of two types: the first arising from `overlap' of dynamic shape transforms and causing smoothly varying modulations of the zero-layer reflections, and the second, caused by direct interactions with the upper-layer, or higher-order Laue zone lines, leading to a sharply defined fine-line structure. These latter interactions are especially useful in increasing the accuracy of space-group determination (Tanaka et al., 1983[link]), and may be enhanced by the use of low-temperature specimen stages. The presence of these defect lines in convergent-beam discs, occurring especially in low-symmetry zone-axis patterns, allows symmetry elements to be related to the three-dimensional structure (Section 2.5.3.5[link]; Fig. 2.5.3.4c[link]).

To the extent that such three-dimensional effects can be ignored or are absent in the zero-layer pattern the projection approximation (Chapter 5.2[link] ) can be applied. This situation most commonly occurs in zone-axis patterns taken from relatively thin crystals and provides a useful starting point for many analyses, by identifying the projected symmetry.

2.5.3.2. Background theory and analytical approach

| top | pdf |

2.5.3.2.1. Direct and reciprocity symmetries: types I and II

| top | pdf |

Convergent-beam diffraction symmetries are those of Schrödinger's equation, i.e. of crystal potential, plus the diffracting electron. The appropriate equation is given in Section 2.5.2[link] [equation (2.5.2.6)[link]] and Chapter 5.2 [equation (5.2.2.1)][link] in terms of the real-space wavefunction ψ. The symmetry elements of the crystal responsible for generating pattern symmetries may be conveniently classified as of two types (I and II) as follows.

  • I. The direct (type I: Table 2.5.3.1[link]) symmetries imposed by this equation on the transmitted wavefunction given z-axis illumination ([{\bf k}_{0}], the incident wavevector parallel to Z, the surface normal) are just the symmetries of ϕ whose operation leaves both crystal and z axis unchanged. These are also called `vertical' symmetry elements, since they contain Z. These symmetries apply equally in real and reciprocal space, since the operator [\nabla^{2}] has circular symmetry in both spaces and does nothing to degrade the symmetry in transmission. Hence, for high-symmetry crystals (zone axis parallel to z axis), and to a greater or lesser degree for crystals of a more general morphology, these zone-axis symmetries apply both to electron-microscope lattice images and to convergent-beam patterns under z-axis-symmetrical illumination, and so impact also on space-group determination by means of high-resolution electron microscopy (HREM). In CBED, these elements lead to whole pattern symmetries, to which every point in the pattern contributes, regardless of diffraction order and Laue zone (encompassing ZOLZ and HOLZ reflections).

    Table 2.5.3.1| top | pdf |
    Listing of the symmetry elements relating to CBED patterns under the classifications of `vertical' (I), `horizontal' (II) and combined or roto-inversionary axes

    I. Vertical symmetry elements
    International symbols
    [\eqalign{&2, 3, 4, 6 \quad (2_{1}, 3_{1}, \ldots)\cr &m \;\phantom{2, 3, 4, 6} (c)\cr &a, b \!\!\phantom{2, 3, 4, 6} (n)}]
    II. Horizontal symmetry elements
      Diperiodic symbols BESR symbols
    2′ m
    [2'_{1}]  
    m [1_{R}]
    a′, b′, n  
    [\bar{1}'] [2_{R}]
    [\hbox{I} + \hbox{II}] [\bar{4}'] [4_{R}]
    [\hbox{I} \times \hbox{II}] [\bar{3}' = 3 \times \bar{1}'] [6_{R} = 3 \cdot 2_{R}]
      [\bar{6}' = 3 \times m'] [31_{R}]
  • II. Reciprocity-induced symmetries, on the other hand, depend upon ray paths and path reversal, and in the present context have relevance only to the diffraction pattern. Crystal-inverting or horizontal crystal symmetry elements combine with reciprocity to yield indirect pattern symmetries lacking a one-to-one real-space correspondence, within individual diffraction discs or between disc pairs. Type II elements are assumed to lie on the central plane of the crystal, midway between surfaces, as symmetry operators; this assumption amounts to a `central plane' approximation, which has a very general validity in space-group-determination work (Goodman, 1984a[link]).

A minimal summary of basic theoretical points, otherwise found in Chapter 5.2[link] and numerous referenced articles, is given here.

For a specific zero-layer diffraction order [g\ (=h, k)] the incident and diffracted vectors are [{\bf k}_{0}] and [{\bf k}_{g}]. Then the three-dimensional vector [{\bf K}_{0g} = {1 \over 2} ({\bf k}_{0} + {\bf k}_{g})] has the pattern-space projection, [{\bf K}_{g} = {}^{p}[{\bf K}_{0g}]]. The point [{\bf K}_{g} = {\bf 0}] gives the symmetrical Bragg condition for the associated diffraction disc, and [{\bf K}_{g} \neq {\bf 0}] is identifiable with the angular deviation of [{\bf K}_{0g}] from the vertical z axis in three-dimensional space (see Fig. 2.5.3.1[link]). [{\bf K}_{g} = {\bf 0}] also defines the symmetry centre within the two-dimensional disc diagram (Fig. 2.5.3.2[link]); namely, the intersection of the lines S and G, given by the trace of excitation error, [{\bf K}_{g} = {\bf 0}], and the perpendicular line directed towards the reciprocal-space origin, respectively. To be definitive it is necessary to index diffracted amplitudes relating to a fixed crystal thickness and wavelength, with both crystallographic and momentum coordinates, as [{\bf u}_{g, \, K}], to handle the continuous variation of [{\bf u}_{g}] (for a particular diffraction order), with angles of incidence as determined by [{\bf k}_{0}], and registered in the diffraction plane as the projection of [{\bf K}_{0g}].

[Figure 2.5.3.1]

Figure 2.5.3.1 | top | pdf |

Vector diagram in semi-reciprocal space, using Ewald-sphere constructions to show the `incident', `reciprocity' and `reciprocity × centrosymmetry' sets of vectors. Dashed lines connect the full vectors [{\bf K}_{0g}] to their projections [{\bf K}_{g}] in the plane of observation.

[Figure 2.5.3.2]

Figure 2.5.3.2 | top | pdf |

Diagrammatic representation of a CBED disc with symmetry lines m, mR (alternate labels G, S) and the central point [{\bf K}_{g} = 0].

2.5.3.2.2. Reciprocity and Friedel's law

| top | pdf |

Reciprocity was introduced into the subject of electron diffraction in stages, the essential theoretical basis, through Schrödinger's equation, being given by Bilhorn et al. (1964)[link], and the N-beam diffraction applications being derived successively by von Laue (1935)[link], Cowley (1969)[link], Pogany & Turner (1968)[link], Moodie (1972)[link], Buxton et al. (1976)[link], and Gunning & Goodman (1992)[link].

Reciprocity represents a reverse-incidence configuration reached with the reversed wavevectors [\bar{{\bf k}}_{0} = - {\bf k}_{g}] and [\bar{{\bf k}}_{g} = - {\bf k}_{0}], so that the scattering vector [\Delta{\bf k} = {\bf k}_{g} - {\bf k}_{0} = \bar{{\bf k}}_{0} - \bar{{\bf k}}_{g}] is unchanged, but [\bar{{\bf K}}_{0g} = {1 \over 2} (\bar{{\bf k}}_{0} + \bar{{\bf k}}_{g})] is changed in sign and hence reversed (Moodie, 1972[link]). The reciprocity equation, [{\bf u}_{g, \, {\bf K}} = {\bf u}_{g, \, \bar{{\bf K}}}^{*}, \eqno(2.5.3.1)] is valid independently of crystal symmetry, but cannot contribute symmetry to the pattern unless a crystal-inverting symmetry element is present (since [\bar{{\bf K}}] belongs to a reversed wavevector). The simplest case is centrosymmetry, which permits the right-hand side of (2.5.3.1)[link] to be complex-conjugated giving the useful CBED pattern equation [{\bf u}_{g, \, {\bf K}} = {\bf u}_{\bar{g}, \, {\bf K}}. \eqno(2.5.3.2)] Since K is common to both sides there is a point-by-point identity between the related distributions, separated by 2g (the distance between g and [\bar{g}] reflections). This invites an obvious analogy with Friedel's law, [F_{g} = F_{\bar{g}}^{*}], with the reservation that (2.5.3.2)[link] holds only for centrosymmetric crystals. This condition (2.5.3.2)[link] constitutes what has become known as the ±H symmetry and, incidentally, is the only reciprocity-induced symmetry so general as to not depend upon a disc symmetry-point or line, nor on a particular zone axis (i.e. it is not a point symmetry but a translational symmetry of the pattern intensity).

2.5.3.2.3. In-disc symmetries

| top | pdf |

  • (a) Dark-field (diffracted-beam) discs . Other reciprocity-generated symmetries which are available for experimental observation relate to a single (zero-layer) disc and its origin [{\bf K}_{g} = {\bf 0}], and are summarized here by reference to Fig. 2.5.3.2[link], and given in operational detail in Table 2.5.3.2[link]. The notation subscript R, for reciprocity-induced symmetries, introduced by Buxton et al. (1976)[link] is now adopted (and referred to as BESR notation). Fig. 2.5.3.2[link] shows a disc crossed by reference lines m and [m_{R}]. These will be mirror lines of intensity if: (a) g is parallel to a vertical mirror plane; and (b) g is parallel to a horizontal diad axis, respectively. The third possible point symmetry, that of disc centrosymmetry ([1_{R}] in BESR notation) will arise from the presence of a horizontal mirror plane. Lines m and [m_{R}] become the GS extinction lines G and S when glide planes and screw axes are present instead of mirror planes and diad axes.

    Table 2.5.3.2| top | pdf |
    Diagrammatic illustrations of the actions of five types of symmetry elements (given in the last column in Volume A diagrammatic symbols) on an asymmetric pattern component, in relation to the centre of the pattern at [{\bf K}_{00} = {\bf 0}], shown as `⊕', or in relation to the centre of a diffraction order at [{\bf K}_{0g} = {\bf 0}], shown as `+'

    TypeSymmetry elementObservation and actionIn combinationInterpretation
    Vertical 4 [Scheme scheme1]   [Scheme scheme2]
    m; a [Scheme scheme3] [Scheme scheme5] [Scheme scheme6]
    Horizontal 2′; [2'_{1}] [Scheme scheme4]
    [i(\bar{1}')] [Scheme scheme7]   [Scheme scheme8]
    m′; a [Scheme scheme9]   [Scheme scheme10]
  • (b) Bright-field (central-beam) disc . The central beam is a special case since the point [{\bf K}_{0} = {\bf 0}] is the centre of the whole pattern as well as of that particular disc. Therefore, both sets of rotational symmetry (types I[link] and II[link]) discussed above apply (see Table 2.5.3.3[link]).

    In addition, the central-beam disc is a source of three-dimensional lattice information from defect-line scattering. Given a sufficiently perfect crystal this fine-line structure overlays the more general intensity modulation, giving this disc a lower and more precisely recorded symmetry.

2.5.3.2.4. Zero-layer absences

| top | pdf |

Horizontal glides, a′, n′ (diperiodic, primed notation), generate zero-layer absent rows, or centring, rather than GS bands (see Fig. 2.5.3.3[link]). This is an example of the projection approximation in its most universally held form, i.e. in application to absences. Other examples of this are: (a) appearance of both G and S extinction bands near their intersection irrespective of whether glide or screw axes are involved; and (b) suppression of the influence of vertical, non-primitive translations with respect to observations in the zero layer. It is generally assumed as a working rule that the zero-layer or ZOLZ pattern will have the rotational symmetry of the point-group component of the vertical screw axis (so that [2_{1} \simeq 2]). Elements included in Table 2.5.3.1[link] on this pretext are given in parentheses. However, the presence of [2_{1}] rather than 2 ([3_{1}] rather than 3 etc.) should be detectable as a departure from accurate twofold symmetry in the first-order-Laue-zone (FOLZ) reflection circle (depicted in Fig. 2.5.3.3[link]). This has been observed in the cubic structure of Ba2Fe2O5Cl2, permitting the space groups I23 and [I2_{1} 3] to be distinguished (Schwartzman et al., 1996[link]). A summary of all the symmetry components described in this section is given diagrammatically in Table 2.5.3.2[link].

[Figure 2.5.3.3]

Figure 2.5.3.3 | top | pdf |

Diagrammatic representation of the influence of non-symmorphic elements: (i) Alternate rows of the zero-layer pattern are absent owing to the horizontal glide plane. The pattern is indexed as for an `a' glide; the alternative indices (in parentheses) apply for a `b' glide. (ii) GS bands are shown along the central row of the zero layer, for odd-order reflections.

2.5.3.3. Pattern observation of individual symmetry elements

| top | pdf |

The following guidelines, the result of accumulated experience from several laboratories, are given in an experimentally based sequence, and approximately in order of value and reliability.

  • (i) The value of X in an X-fold rotation axis is made immediately obvious in a zone-axis pattern, although a screw component is not detected in the pattern symmetry.

    Roto-inversionary axes require special attention: [\bar{6}] and [\bar{3}] may be factorized, as in Tables 2.5.3.1[link], 2.5.3.3[link] and 2.5.3.4[link], to show better the additional CBED symmetries ([3/m'] and [3 \times \bar{1}'], respectively). [\bar{4}] cannot be decomposed further (Table 2.5.3.1)[link] and generates its own diffraction characteristics in non-projective patterns (see Section 2.5.3.5[link]). This specific problem of observing the fourfold roto-inversion symmetry has been resolved recently by Tanaka et al. (1994)[link] using both CBED and LACBED techniques.

  • (ii) Vertical mirror plane determination may be the most accurate crystal point-symmetry test, given that it is possible to follow the symmetry through large crystal rotations (say 5 to 15°) about the mirror normal. It is also relatively unaffected by crystal surface steps as compared to (v)[link] below.

  • (iii) Horizontal glide planes are determined unequivocally from zero-layer absences when the first Laue zone is recorded, either with the main pattern or by further crystal rotation; i.e. a section of this zone is needed to determine the lateral unit-cell parameters. This observation is illustrated diagrammatically in Fig. 2.5.3.3[link].

  • (iv) An extinction (GS) line or band through odd-order reflections of a zone-axis pattern indicates only a projected glide line . This is true because both [P2_{1}] (No. 4) and Pa (No. 7) symmetries project into `pg' in two dimensions. However, the projection approximation has only limited validity in CBED. For all crystal rotations around the [2_{1}] axis, or alternatively about the glide-plane `a' normal, dynamic extinction conditions are retained. This is summarized by saying that the diffraction vector [{\bf K}_{0{g}}] should be either normal to a screw axis or contained within a glide plane for the generation of the S or G bands, respectively. Hence [P2_{1}] and Pa may be distinguished by these types of rotations away from the zone axis with the consequence that the element [2_{1}] in particular is characterized by extinctions close to the Laue circle for the tilted ZOLZ pattern (Goodman, 1984b[link]), and that the glide a will generate extinction bands through both ZOLZ and HOLZ reflections for all orientations maintaining Laue-circle symmetry about the S band (Steeds et al., 1978[link]).

    As a supplement to this, in a refined technique not universally applicable, Tanaka et al. (1983)[link] have shown that fine-line detail from HOLZ interaction can be observed which will separately identify S- (21) and G-band symmetry from a single pattern (see Fig. 2.5.3.6[link]).

  • (v) The centre-of-symmetry (or ±H) test can be made very sensitive by suitable choice of diffraction conditions but requires a reasonably flat crystal since it involves a pair of patterns (the angular beam shift involved is very likely to be associated with some lateral probe shift on the specimen). This test is best carried out at a low-symmetry zone axis, free from other symmetries, and preferably incorporating some fine-line HOLZ detail, in the following way. The hkl and [\bar{h}\bar{k}\bar{l}] reflections are successively illuminated by accurately exchanging the central-beam aperture with the diffracted-beam apertures, having first brought the zone axis on to the electron-microscope optic axis. This produces the symmetrical ±H condition.

  • (vi) In seeking internal [m_{R}] symmetry as a test for a horizontal diad axis it is as well to involve some distinctive detail in the mirror symmetry (i.e. simple two-beam-like fringes should be avoided), and also to rotate the crystal about the supposed diad axis, to avoid an [m_{R}] symmetry due to projection [for examples see Fraser et al. (1985)[link] and Goodman & Whitfield (1980)[link]].

  • (vii) The presence or absence of the in-disc centrosymmetry element [1_{R}] formally indicates the presence or absence of a horizontal mirror element m′, either as a true mirror or as the mirror component of a horizontal glide plane g′. In this case the absence of symmetry provides more positive evidence than its presence, since absence is sufficient evidence for a lack of central-mirror crystal symmetry but an observed symmetry could arise from the operation of the projection approximation. If some evidence of the three-dimensional interaction is included in the observation or if three-dimensional interaction (from a large c axis parallel to the zone axis) is evident in the rest of the pattern, this latter possibility can be excluded. Interpretation is also made more positive by extending the angular aperture, especially by the use of LACBED.

These results are illustrated in Table 2.5.3.2[link] and by actual examples in Section 2.5.3.5[link].

2.5.3.4. Auxiliary tables

| top | pdf |

Space groups may very well be identified using CBED patterns from an understanding of the diffraction properties of real-space symmetry elements, displayed for example in Table 2.5.3.2[link]. It is, however, of great assistance to have the symmetries tabulated in reciprocal space, to allow direct comparison with the pattern symmetries.

There are three generally useful ways in which this can be done, and these are set out in Tables 2.5.3.3[link] [link] to 2.5.3.5[link]. The simplest of these is by means of point group, following the procedures of Buxton et al. (1976)[link]. Next, the CBED pattern symmetries can be listed as diperiodic groups which are space groups in two dimensions, allowing identification with a restricted set of three-dimensional space groups (Goodman, 1984b[link]). Finally, the dynamic extinctions (GS bands and zero-layer absences) can be listed for each non-symmorphic space group, together with the diffraction conditions for their observation (Tanaka et al., 1983[link]; Tanaka & Terauchi, 1985[link]). Descriptions for these tables are given below.

  • Table 2.5.3.3[link]. BESR symbols (Buxton et al., 1976[link]) incorporate the subscript R to describe reciprocity-related symmetry elements, R being the operator that rotates the disc pattern by 180° about its centre. The symbols formed in this way are [1_{R}], [2_{R}], [4_{R}], [6_{R}], where [X_{R}] represents [2\pi / X] rotation about the zone axis, followed by R. Of these, [2_{R}] represents the ±H symmetry (two twofold rotations) described earlier [equation (2.5.3.2)[link]] as a transformation of crystalline centrosymmetry; [6_{R}] may be thought of as decomposing into [3 \cdot 2_{R}] for purposes of measurement. The mirror line [m_{R}] (Fig. 2.5.3.2[link]) is similarly generated by [m \cdot 1_{R}].

    Table 2.5.3.3| top | pdf |
    Diffraction point-group tables, giving whole-pattern and central-beam pattern symmetries in terms of BESR diffraction-group symbols and diperiodic group symbols

    IIIIIIIVV
    Whole patternBright field (central beam)BESR groupDiperiodic group (point group)Cubic point groups
    [100][110]
    1 1 1 1    
    1 2 [\underline{1}_{R}] m    
    2 2 2 2    
    1 1 [^{*}2_{R}] [{\bar 1}^{\prime}]    
    2 2 [^{*}\underline{21}_{R}] [2/m']    
    1 m [m_{R}] 2′   23
    m m m m    
    m 2mm [\underline{m1}_{R}] 2′mm   [\bar{4}3m]
    2 2mm [2m_{R}m_{R}] 2′2′2 23 432
    2mm 2mm 2mm mm2    
    m m [^{*}2_{R}mm_{R}] [2'/m]   m [\bar{3}]
    2mm 2mm [^{*}\underline{2mm1}_{R}] mmm m [\bar{3}] m [\bar{3}] m
    4 4 4 4    
    2 4 [4_{R}] [\bar{4}']    
    4 4 [^{*}\underline{41}_{R}] [4/m']    
    4 4mm [4m_{R}m_{R}] [42'2'] [\bar{4}32]  
    4mm 4mm 4mm 4mm    
    2mm 4mm [4_{R}mm_{R}] [\bar{4}'m2'] [\bar{4}3m]  
    4mm 4mm [^{*}\underline{4mm1}_{R}] [4/m'mm] m [\bar{3}] m  
    3 3 3 3    
    3 6 [\underline{31}_{R}] [\bar{6}']    
    3 3m [3m_{R}] 32′    
    3m 3m 3m 3m    
    3m 6mm [\underline{3m1}_{R}] [\bar{6}'m2']    
    6 6 6 6    
    3 3 [^{*}6_{R}] [\bar{3}']    
    6 6 [^{*}\underline{61}_{R}] [6/m']    
    6 6mm [6m_{R}m_{R}] [62'2']    
    6mm 6mm 6mm 6mm    
    3m 3m [^{*}6_{R}mm_{R}] [\bar{3}'m]    
    6mm 6mm [^{*}\underline{6mm1}_{R}] [6/m'mm]    

    Table 2.5.3.3[link] gives the BESR interrelation of pattern symmetries with point group (Buxton et al., 1976[link]; Steeds, 1983[link]). Columns I and II of the table list the point symmetries of the whole pattern and bright-field pattern, respectively; column III gives the BESR diffraction groups. [Note: following the Pond & Vlachavas (1983)[link] usage, `[^{*}]' has been appended to the centrosymmetric groups.]

    Inspection of columns I and II shows that 11 of the 31 diffraction groups can be determined from a knowledge of the whole pattern and bright-field (central-beam disc) point symmetries alone. The remaining 10 pairs of groups need additional observation of the dark-field pattern for their resolution. Disc symmetries [1_{R}], [m_{R}] (Fig. 2.5.3.2[link]; Table 2.5.3.2[link]) are sought (a) in general zero-layer discs and (b) in discs having an [m_{R}] line perpendicular to a proposed twofold axis, respectively; the ±H test is applied for centrosymmetry, to complete the classification.

    Column IV gives the equivalent diperiodic point-group symbol, which, unprimed, gives the corresponding three-dimensional symbol. This will always refer to a non-cubic point group. Column V gives the additional cubic point-group information indicating, where appropriate, how to translate the diffraction symmetry into [100] or [110] cubic settings, respectively.

    Of the groups listed in column III, those representing the projection group of their class are underlined. These groups all contain [1_{R}], the BESR symbol for m′. When the projection approximation is applicable, only those groups underlined will apply. The effect of this approximation is to add a horizontal mirror plane to the symmetry group.

  • Table 2.5.3.4[link]. This lists possible space groups for each of the classified zero-layer CBED symmetries. Since the latter constitute the 80 diperiodic groups, it is first necessary to index the pattern in diperiodic nomenclature; the set of possible space groups is then given by the table.

    Table 2.5.3.4| top | pdf |
    Tabulation of principal-axis CBED pattern symmetries against relevant space groups given as IT A numbers

    Three columns of diperiodic groups (central section) correspond to (i) symmorphic groups, (ii) non-symmorphic groups (GS bands) and (iii) non-symmorphic groups (zero-layer absences arising from horizontal glide planes). Cubic space groups are given underlined in the right-hand section with the code: underlining = [001](cyclic) setting; italics + underlining = [110](cyclic) setting. Separators `;' and `:' indicate change of Bravais lattice type and change of crystal system, respectively.

    DGIIISGIII
    Point groupsDiperiodic groupsSpace groups
    H–MBESR(i)(ii)(iii)Subgroups IIb (Subgroups 1)
    Oblique Triclinic
    1 1 1 p1     1  
    2* [\bar{1}] [2_{R}] [p\bar{1}']     2  
      Monoclinic (Oblique)
    3 12 2 p2     3 4, 5
    4 1m [1_{R}] pm     6 8
    5 1m       pb 7 9
    6* [2/m] [21_{R}] [p2/m']     10 11, 12
    7* [2/m] [21_{R}]     [p2/b'] 13 14, 15
    Rectangular (Rectangular)
    8 21 [m_{R}] p2′     3 52: 195; 197, 199
    9 21 [m_{R}]   [p2'_{1}]   4 198
    10 21 [m_{R}] c2′     5 196
    11 m1 m pm     62 7, 82
    12 m1 m   pa   72 92
    13 m1 m cm     8 9
    14* [12/m] [2_{R}mm_{R}] [p2'/m]     10 13, 122: 200, 201; 204
    15* [12/m] [2_{R}mm_{R}]   [p2'_{1}/m]   11 14
    16* [12/m] [2_{R}mm_{R}]   [p2'/a]   132 152: 206
    17* [12/m] [2_{R}mm_{R}]   [p2'_{1}/m]   142 205
    18* [12/m] [2_{R}mm_{R}] [c2'/m]     12 15: 202, 203
      Orthorhombic
    19 222 [2m_{R}m_{R}] p2′2′2     16 17; 212; 22: 195; 196, 207, 206; 211, 214
    20 222 [2m_{R}m_{R}]   [p2'_{1}2'2]   172 182; 202: 212, 213
    21 222 [2m_{R}m_{R}]   [p2'_{1}2'_{1}2]   18 19: 198
    22 222 [2m_{R}m_{R}] c2′2′2     21 20; 23, 24: 197, 199, 209, 210
    23 mm2 2mm pmm2     25 26, 27; 38, 39; 42
    24 mm2 2mm   pbm2   28 29, 30, 312; 40, 41
    25 mm2 2mm   pba2   32 33, 34; 43
    26 mm2 2mm cmm2     35 36, 37; 44, 45, 46
    27 mm2 [m1_{R}] p2′mm     252 281; 352, 422; 382, 392: 215; 217
    28 mm2 [m1_{R}]   [p2'_{1}m'a]   261 311; 361
    29 mm2 [m1_{R}]   [p2'_{1}ab'] [(p2'_{1}ab')] 292 332
    30 mm2 [m1_{R}]     [p2'_{1}ma'] 262 291; 362
    31 mm2 [m1_{R}]     [p2'_{1}mn'] 312 332
    32 mm2 [m1_{R}]     p2′mb 282 322, 402, 412
    33 mm2 [m1_{R}]     p2′aa 272 302; 372
    34 mm2 [m1_{R}]     [pb2'n'] 301 342; 432: 218; 219
    35 mm2 [m1_{R}] c2′mm     381 401; 442, 461: 216; 220
    36 mm2 [m1_{R}]     c2′mb 391 411; 452, 462
    37* mmm [2mm1_{R}] pmmm     47 49, 511; 652, 672; 69:
                  200; 202, 221, 224, 226, 228, 229
    38* mmm [2mm1_{R}]   [pbmm'\; (2'_{1})]   512 531, 57, 592; 631, 641
    39* mmm [2mm1_{R}]   [pbam'\; (2'_{1}2'_{1})]   55 58, 622
    40* mmm [2mm1_{R}]   [pmab'\; (2'_{1}2'_{1})] [(pmab')] 571 602, 61, 62: 205
    41* mmm [2mm1_{R}]   [pbaa'\; (2'_{1})] [(pbaa')] 542 52, 562, 601
    42* mmm [2mm1_{R}]     [pmma'\; (2'_{1})] 51 54, 552, 572; 632, 642
    43* mmm [2mm1_{R}]     pmmn[(2'_{1}2'_{1})] 59 56, 621
    44* mmm [2mm1_{R}]     [pbmn'\; (2'_{1})] 532 521, 581, 60
    45* mmm [2mm1_{R}]     pmaa 492 502, 53, 541; 662, 681: 222, 223
    46* mmm [2mm1_{R}]     pban 50 522, 48; 70: 201; 203, 230
    47* mmm [2mm1_{R}] cmmm     65 63, 66; 72, 742, 71: 204, 225, 227
    48* mmm [2mm1_{R}]     cmma 67 64, 68; 721, 74, 73: 206
    Square Tetragonal
    49 4 4 p4     75 77, 76, 78; 79, 80
    50 [4/m] [41_{R}] [p4/m']     83 84; 87
    51 [4/m] [41_{R}]     [p4/n'] 85 86, 88
    52 422 [4m_{R}m_{R}] [p42'2']     89 93, 91, 95; 97, 98:
                  207, 208; 209, 210; 211, 214
    53 422 [4m_{R}m_{R}]   [p42'_{1}2']   90 94, 92, 96: 212, 213
    54 4mm 4mm [p4mm]     99 101, 103, 105; 107, 108
    55 4mm 4mm   [p4bm]   100 102, 104, 106; 109, 110
    56* [4/mmm] [4mm1_{R}] [p4/m'mm]     123 124, 131, 132; 139, 140;
                  221, 223; 225, 226; 229
    57* [4/mmm] [4mm1_{R}]   [p4/m'bm\; (2'_{1})]   127 128, 135, 136
    58* [4/mmm] [4mm1_{R}]     [p4/n'bm] 125 126, 133, 134; 141, 142:
                  222, 224; 227, 228; 230
    59* [4/mmm] [4mm1_{R}]     [p4/n'mm\; (2'_{1})] 129 130, 137, 138
    60 [\bar{4}] [4_{R}] [p\bar{4}']     81 82
    61 [\bar{4}2m] [4_{R}mm_{R}] [p\bar{4}'m\bar{2}']     115 116; 119, 120
    62 [\bar{4}2m] [4_{R}mm_{R}]   [p\bar{4}b2']   117 118; 122: 220
    63 [\bar{4}2m] [4_{R}mm_{R}] [p\bar{4}'2'm]     111 112; 121: 215; 216; 217; 218; 219
    64 [\bar{4}2m] [4_{R}mm_{R}]   [p4'2'_{1}m]   113 114
    Hexagonal Trigonal
    65 3 3 p3     143 144, 145; 146
    66 [\bar{3}] [6_{R}] [p\bar{3}']     147 148
    67 32 [3m_{R}] p312′     149 151, 153
    68 32 [3m_{R}] [p32'1]     150 152, 154; 155
    69 3m 3m p31m     157 159
    70 3m 3m p3m1     156 158; 160, 161
    71* [\bar{3}m] [6_{R}mm_{R}] [p\bar{3}'1m]     162 163
    72* [\bar{3}m] [6_{R}mm_{R}] [p\bar{3}'m1]     164 165; 166, 167
      Hexagonal
    73 6 6 p6     168 171, 172, 173, 169, 170
    74 [\bar{6}] [31_{R}] [p3/m'\; (p\bar{6}')]     174  
    75 622 [6m_{R}m_{R}] [p62'2']     177 180, 181, 182, 178, 179
    76 6mm 6mm [p6mm]     183 184, 185, 186
    77* [6/m] [61_{R}] [p6/m']     175 176
    78* [6/mmm] [6mm1_{R}] [p6/m'mm]     191 192, 193, 194
    79 [\bar{6}m2] [3m1_{R}] [p3/m'2'm] [(p\bar{6}'m2')]     189 190
    80 [\bar{6}m2] [3m1_{R}] [p3/m'm2'] [(p\bar{6}'2'm)]     187 188

    A basic requirement for diperiodic group nomenclature has been that of compatibility with IT A and I. This has been met by the recent Pond & Vlachavas (1983) tabulation. For example, DG: [(^{*})pban'], where [^{*}] indicates centrosymmetry, becomes space group Pban when, in Seitz matrix description, the former group matrix is multiplied by the third primitive translation, [a_{3}]. Furthermore, in textual reference the prime can be optionally omitted, since the lower-case lattice symbol is sufficient indication of a two-dimensional periodicity (as pban).

    The three sections of Table 2.5.3.4[link] are:

    • I. Point-group entries, given in H–M and BESR symbols.

    • II. Pattern symmetries, in diperiodic nomenclature, have three subdivisions: (i) symmorphic groups: patterns without zero-layer absences or extinctions. Non-symmorphic groups are then given in two categories: (ii) patterns with zero-layer GS bands, and (iii) patterns with zero-layer absences resulting from a horizontal glide plane; where the pattern also contains dynamic extinctions (GS bands) and so is listed in column (ii), the column (iii) listing is given in parentheses.

      The `short' (Pond & Vlachavas) symbol has proved an adequate description for all but nine groups for which the screw-axis content was needed: here [(2'_{1})], or [(2'_{1}2'_{1})], have been added to the symbol.

    • III. Space-group entries are given in terms of IT A numbers. The first column of each row gives the same-name space group as illustrated by the example pban′ → Pban above. The groups following in the same row (which have the same zero-layer symmetry) complete an exhaustive listing of the IIb subgroups, given in IT A . Cubic space groups are underlined for the sake of clarity; hence, those giving rise to the zero-layer symmetry of the diffraction group in the [100] (cyclic) setting have a single underline: these are type I minimal supergroups in IT A nomenclature. The cubic groups are also given in the [110] setting, in underlined italics, since this is a commonly encountered high-symmetry setting. (Note: these then are no longer minimal supergroups and the relationship has to be found through a series of IT A listings.)

    The table relates to maximal-symmetry settings. For monoclinic and orthorhombic systems there are three equally valid settings. For monoclinic groups, the oblique and rectangular settings appear separately; where rectangular C-centred groups appear in a second setting this is indicated by superscript `2'. For orthorhombic groups, superscripts correspond to the `incident-beam' system adopted in Table 2.5.3.5[link], as follows: no superscript: [001] beam direction; superscript 1: [100] beam direction; superscript 2: [010] beam direction. The cubic system is treated specially as described above.

    Table 2.5.3.5| top | pdf |
    Conditions for observation of GS bands for the 137 space groups exhibiting these extinctions

    Point groups 2, m, [{\bf 2/{\bi m}}] (2nd setting unique axis [\|b])

    Space groupIncident-beam direction
    [u0w]
    4 [P2_{1}] 0k0 S
    [2_{1}]
    7 Pc h0l G
    c
    9 Cc h0l G
    c
    11 [P2_{1}/m] 0k0 S
    [2_{1}]
    13 [P2/c] h0l G
    c
    14 [P2_{1}/c] 0k0 S
    [2_{1}]
    h0l G
    c
    15 [C2/c] h0l G
    c

    Point groups 222, mm2

    Space groupIncident-beam direction
    [100][010][001][uv0][0vw][u0w]
    17 [P222_{1}] 00l S 00l S     00l S        
    [2_{1}] [2_{1}]     [2_{1}]        
    18 [P2_{1}2_{1}2] 0k0 S h00 S h00, 0k0 S     h00 S 0k0 S
    [2_{1}] [2_{1}] [2_{1}]     [2_{1}] [2_{1}]
    19 [P2_{1}2_{1}2_{1}] 0k0, 00l S h00, 00l S h00, 0k0 S 00l S h00 S 0k0 S
    [2_{1}] [2_{1}] [2_{1}] [2_{1}] [2_{1}] [2_{1}]
    20 [C222_{1}] 00l S 00l S     00l S        
    [2_{1}] [2_{1}]     [2_{1}]        
    26 [Pmc2_{1}] 00l GS 00l c′−     00l S     h0l G
    [c + 2_{1}] [2_{1}]     [2_{1}]     c
    27 [Pcc2] 00l c′− 00l c′−         0kl G h0l G
    c c         c c
    28 [Pma2]         h00 G         h0l G
            a         a
    29 [Pca2_{1}] 00l c′− 00l GS h00 G 00l S 0kl G h0l G
    [2_{1}] [c + 2_{1}] a [2_{1}] c a
    30 [Pnc2] 00l n′− 00l c′− 0k0 G     0kl G h0l G
    c n n     n c
    31 [Pmn2_{1}] 00l GS 00l n′− h00 G 00l S     h0l G
    [n + 2_{1}] [2_{1}] n [2_{1}]     n
    32 [Pba2]         h00, 0k0 G     0kl G h0l G
            a b     b a
    33 [Pna2_{1}] 00l n′− 00l GS h00, 0k0 G 00l S 0kl G h0l G
    [2_{1}] [n + 2_{1}] a b [2_{1}] n a
    34 [Pnn2] 00l n′− 00l n′− h00, 0k0 G     0kl G h0l G
    n n n     n n
    36 [Cmc2_{1}] 00l GS 00l c′−     00l S     h0l G
    [c + 2_{1}] [2_{1}]     [2_{1}]     c
    37 [Ccc2] 00l c′− 00l c′−         0kl G h0l G
    c c         c c
    39 [Abm2]                 0kl G    
                    b    
    40 [Ama2]         h00 G         h0l G
            a         a
    41 [Aba2]         h00 G     0kl G h0l G
            a     b a
    43 [Fdd2] 00l d′− 00l d′− h00, 0k0 G     0kl G h0l G
      d d     d d
    45 [Iba2]   b′−   a′−         0kl G h0l G
                b a
    46 [Ima2]       a′−             h0l G
                      a

    Point group mmm

    Space groupIncident-beam direction
    [100][010][001][uv0][0vw][u0w]
    48 P 2/n 2/n 2/n 00l, 0k0 n′− 00l, h00 n′− 0k0, h00 n′− hk0 G 0kl G h0l G
    n n n n n n
    49 P 2/c 2/c 2/m 00l c′− 00l c′−         0kl G h0l G
    c c         c c
    50 P 2/b 2/a 2/n 0k0 b′− h00 a′− 0k0, h00 n′− hk0 G 0kl G h0l G
    n n b a n b a
    51 [P\;2_{1}/m\;2/m\;2/a]     h00 GS h00 a′− hk0 G h00 S    
        [a + 2_{1}] [2_{1}] a [2_{1}]    
    52 [P\;2/n\;2_{1}/n\;2/a] 00l, 0k0 a′− 00l, h00 n′− 0k0 GS hk0 G 0kl G h0l G
    [n + 2_{1}] n
    [2_{1}] n a h00 n′− a n 0k0 S
    n [2_{1}]
    53 [P\;2/m\;2/n\;2_{1}/a] 00l GS h00, 00l a′− h00 a′− hk0 G     h0l G
    a
    [n + 2_{1}] [2_{1}] n 00l S n
    [2_{1}]
    54 [P\;2_{1}/c\;2/c\;2/a] 00l c′− h00 GS h00 a′− hk0 G 0kl G h0l G
    [a + 2_{1}] c
    c 00l c′− [2_{1}] a h00 S c
    c [2_{1}]
    55 [P\;2_{1}/b\;2_{1}/a\;2/m] 0k0 b′− h00 a′− 0k0, h00 GS     0kl G h0l G
    b a
    [2_{1}] [2_{1}] [b + 2_{1}, a + 2_{1}] h00 S 0k0 S
    [2_{1}] [2_{1}]
    56 [P\;2_{1}/c\;2_{1}/c\;2/n] 0k0 GS h00 GS 0k0, h00 n′− hk0 G 0kl G h0l G
    [n + 2_{1}] [n + 2_{1}] c c
    00l c′− 00l c′− [2_{1}] n h00 S 0k0 S
    c c [2_{1}] [2_{1}]
    57 [P\;2/b\;2_{1}/c\;2_{1}/m] 00l GS 00l c′− 0k0 GS 00l S 0kl G h0l G
    [c + 2_{1}] c
    0k0 b′− [2_{1}] [b + 2_{1}] [2_{1}] b 0k0 S
    [2_{1}] [2_{1}]
    58 [P\;2_{1}/n\;2_{1}/n\;2/m] 00l, 0k0 n′− 00l, h00 n′− 0k0, h00 GS     0kl G h0l G
    n n
    n [2_{1}] n [2_{1}] [n + 2_{1}] h00 S 0k0 S
    [2_{1}] [2_{1}]
    59 [P\;2_{1}/m\;2_{1}/m\;2/n] 0k0 GS h00 GS 0k0, h00 n′− hk0 G h00 S 0k0 S
    [n + 2_{1}] [n + 2_{1}] [2_{1}] n [2_{1}] [2_{1}]
    60 [P\;2_{1}/b\;2/c\;2_{1}/n] 00l GS h00 GS 0k0, h00 n′− hk0 G 0kl G h0l G
    [c + 2_{1}] [n + 2_{1}] n b
    0k0 b′− 00l c′− b [2_{1}] 00l S h00 S c
    n [2_{1}] [2_{1}] [2_{1}]
    61 [P\;2_{1}/b\;2_{1}/c\;2_{1}/a] 00l GS h00 GS 0k0 GS hk0 G 0kl G h0l G
    [c + 2_{1}] [a + 2_{1}] [b + 2_{1}] a b c
    0k0 b′− 00l c′− h00 a′− 00l S h00 S 0k0 S
    [2_{1}] [2_{1}] [2_{1}] [2_{1}] [2_{1}] [2_{1}]
    62 [P\;2_{1}/n\;2_{1}/m\;2_{1}/a] 0k0, 00l n′− 00l GS 0k0 GS hk0 G 0kl G 0k0 S
    [n + 2_{1}] [n + 2_{1}] a n
    [2_{1}] h00 h00 a′− 00l S h00 S [2_{1}]
    [a + 2_{1}] [2_{1}] [2_{1}] [2_{1}]
    63 [C\;2/m\;2/c\;2_{1}/m] 00l GS 00l c′−     00l S     h0l G
    [c + 2_{1}] [2_{1}] [2_{1}] c
    64 [C\;2/m\;2/c\;2_{1}/a] 00l GS 00l c′−     hk0 G     h0l G
    a
    [c + 2_{1}] [2_{1}] 00l S c
    [2_{1}]
    66 [C\;2/c\;2/c\;2/m] 00l c′− 00l c′−         0kl G h0l G
    c c c c
    67 [C\;2/m\;2/m\;2/a]             hk0 G        
    a
    68 [C\;2/c\;2/c\;2/a] 00l c′− 00l c′−     hk0 G 0kl G h0l G
    c c a c c
    70 [F\;2/d\;2/d\;2/d] 00l, 0k0 d′− h00, 00l d′− 0k0, h00 d′− hk0 G 0kl G h0l G
    d d d d d d
    72 [I\;2/b\;2/a\;2/m]   b′−   a′−         0kl G h0l G
    b a
    73 [I\;2/b\;2/c\;2/a]   b′−   c′−   a′− hk0 G 0kl G h0l G
    a b c
    74 [I\;2/m\;2/m\;2/a]             hk0 G        
    a

    Point groups 4, [\bar{\bf{4}}], [\bf{4/{\bi m}}]

    Space groupIncident-beam direction
    [uv0]
    76 [P4_{1}] 00l S
    [4_{1}]
    78 [P4_{3}] 00l S
    [4_{3}]
    85 [P4/n] hk0 G
    n
    86 [P4_{2}/n] hk0 G
    n
    88 [I4_{1}/a] hk0 G
    a

    Point group 422

    Space groupIncident-beam direction
    [uv0][0vw]
    90 [P42_{1}2]     h00 S
    [2_{1}]
    91 [P4_{1}22] 00l S    
    [4_{1}]
    92 [P4_{1}2_{1}2] 00l S h00 S
    [4_{1}] [2_{1}]
    94 [P4_{2}2_{1}2]     h00 S
    [2_{1}]
    95 [P4_{3}22] 00l S    
    [4_{3}]
    96 [P4_{3}2_{1}2] 00l S h00 S
    [4_{3}] [2_{1}]

    Point group 4mm

    Space groupIncident-beam direction
    [100][001][110][u0w] and [0vw] [[u\bar{u}w]]
    100 [P4bm]     h00, 0k0 G     h0l, 0kl G    
    a b a b
    101 [P4_{2}cm] 00l c′−         h0l, 0kl G    
    c c
    102 [P4_{2}nm] 00l n′− h00, 0k0 G     h0l, 0kl G    
    n n n
    103 [P4cc] 00l c′−     00l c′− h0l, 0kl G hhl G
    c c c c
    104 [P4nc] 00l n′− h00, 0k0 G 00l c′− h0l, 0kl G hhl G
    n n c n c
    105 [P4_{2}mc]         00l c′−     hhl G
    c c
    106 [P4_{2}bc]     h00, 0k0 G 00l c′− h0l, 0kl G hhl G
    a b c a b c
    108 [I4cm]             h0l, 0kl G    
    c
    109 [I3_{1}md]     hh0 G 00l d′−     hhl G
    [{\bar h}h0]    
    d d d
    110 [I4_{1}cd]     hh0 G 00l d′− h0l, 0kl G hhl G
    [{\bar h}h0]      
    d d c d

    Point groups [\bar{\bf {4}}]2m, 4/mmm

    Space groupIncident-beam direction
    [100][001][110][u0w] and [0vw][uuw][uv0]
    112 [P{\bar 4}2c]         00l c′−     hhl G    
    c c
    113 [P{\bar 4}2_{1}m] 0k0 S h00, 0k0 S     0k0, h00 S        
    [2_{1}] [2_{1}] [2_{1}]
    114 [P{\bar 4}2_{1}c] 0k0 S h00, 0k0 S 00l c′− 0k0, h00 S hhl G    
    [2_{1}] [2_{1}] c [2_{1}] c
    116 [P{\bar 4}c2] 00l c′−         h0l, 0kl G        
    c c
    117 [P{\bar 4}b2]     h00, 0k0 G     h0l, 0kl G        
    a b a b
    118 [P{\bar 4}n2] 00l n′− h00, 0k0 G     h0l, 0kl G        
    n n n
    120 [I{\bar 4}c2]             h0l, 0kl G        
    c
    122 [I{\bar 4}2d]     hh0 G 00l d′−     hhl G    
    [{\bar h}h0]    
    d d d
    124 [P\;4/m\;2/c\;2/c] 00l c′−     00l c′− h0l, 0kl G hhl G    
    c c c c
    125 [P\;4/n\;2/b\;2/m] 0k0 b′− h00, 0k0 n′−     h0l, 0kl G     hk0 G
    n a b a b n
    126 [P\;4/n\;2/n\;2/c] 0k0 n′− h00, 0k0 n′− 00l c′− h0l, 0kl G hhl G hk0 G
    n
    00l n c n c n
    n
    127 [P\;4/m\;2_{1}/b\;2/m] 0k0 b′− h00 GS     h0l, 0kl G        
    [a + 2_{1}] a b
    [2_{1}] 0k0 0k0, h00 S
    [b + 2_{1}] [2_{1}]
    128 [P\;4/m\;2_{1}/n\;2/c] 00l, 0k0 n′− h00, 0k0 GS 00l c′− h0l, 0kl G hhl G    
    n
    [n\quad 2_{1}] [n + 2_{1}] c 0k0, h00 S c
    [2_{1}]
    129 [P\;4/n\;2_{1}/m\;2/m] 0k0 GS h00, 0k0 n′−     0k0, h00 S     hk0 G
    [n + 2_{1}] [2_{1}] [2_{1}] n
    130 [P\;4/n\;2_{1}/c\;2/c] 0k0 GS h00, 0k0 n′− 00l c′− h0l, 0kl G hhl G hk0 G
    [n + 2_{1}] c
    00l c′− [2_{1}] c 0k0, h00 S c n
    c [2_{1}]
    131 [P\;4_{2}/m\;2/m\;2/c]         00l c     hhl G    
    c c
    132 [P\;4_{2}/m\;2/c\;2/m] 00l c′−         h0l, 0kl G        
    c c
    133 [P\;4_{2}/n\;2/b\;2/c] 0k0 b′− h00, 0k0 n′− 00l c′− h0l, 0kl G hhl G hk0 G
    n a b c a b c n
    134 [P\;4_{2}/n\;2/n\;2/m] 0k0, 00l n′− h00, 0k0 n′−     h0l, 0kl G     hk0 G
    n n n n
    135 [P\;4_{2}/m\;2_{1}/b\;2/c] 0k0 b′− h00, 0k0 GS 00l c′− h0l, 0kl G hhl G    
    a b
    [2_{1}] [a + 2_{1}\;b + 2_{1}] c 0k0, h00 S c
    [2_{1}]
    136 [P\;4_{2}/m\;2_{1}/n\;2/m] 00l, 0k0 n′− h00, 0k0 GS     h0l, 0kl G        
    n
    n 2 [n + 2_{1}] 0k0, h00 S
    [2_{1}]
    137 [P\;4_{2}/n\;2_{1}/m\;2/c] 0k0 GS h00, 0k0 n′− 00l c′− 0k0, h00 S hhl G hk0 G
    [n + 2_{1}] [2_{1}] c [2_{1}] c n
    138 [P\;4_{2}/n\;2_{1}/c\;2/m] 0k0 GS h00, 0k0 n′−     h0l, 0kl G     hk0 G
    [n + 2_{1}] c
    00l c′− [2_{1}] 0k0, h00 S n
    c [2_{1}]
    140 [I\;4/m\;2/c\;2/m]             h0l, 0kl G        
    c
    141 [I\;4_{1}/a\;2/m\;2/d]     hh0 a′− 00l, [{\bar h}h0] d′−     hhl G hk0 G
    [{\bar h}h0]      
    d d a d a
    142 [I\;4_{1}/a\;2/c\;2/d]     hh0 a′− 00l, [{\bar h}h0] d′− h0l, 0kl G hhl G hk0 G
    [{\bar h}h0]        
    d d a c d a

    Point groups 3m, [{\bar{\bf 3}}] m, 6, 6/m, 622, 6mm, [{\bar{\bf 6}}] m2, 6/mmm

    Space groupIncident-beam direction
    [100][210][2u u w][v0w]
    158 P3c1     000l G [hh2{\bar h}l] G    
    c c
    159 P31c 000l G         [h{\bar h}0l] G
    c c
    161 R3c     000l G [hh2{\bar h}l] G    
    [l = 6n + 3]  
    c c
    163 [P{\bar 3}1c] 000l G         [h{\bar h}0l] G
    c c
    165 [P{\bar 3}c1]     000l G [hh2{\bar h}l] G    
    c c
    167 [R{\bar 3}c]     000l G [hh2{\bar h}l] G    
    [l = 6n + 3]  
    c c
    169 [P6_{1}] 000l S 000l S        
    [6_{1}] [6_{1}]
    170 [P6_{5}] 00l S 00l S        
    [6_{5}] [6_{5}]
    173 [P6_{3}] 000l S 000l S        
    [6_{3}] [6_{3}]
    176 [P6_{3}/m] 000l S 000l S        
    [6_{3}] [6_{3}]
    178 [P6_{1}22] 000l S 000l S        
    [6_{1}] [6_{1}]
    179 [P6_{5}22] 000l S 000l S        
    [6_{5}] [6_{5}]
    182 [P6_{3}22] 000l S 000l S        
    [6_{3}] [6_{3}]
    184 [P6cc] 000l c′− 000l c′− [hh2{\bar h}l] G [h{\bar h}0l] G
    c c c c
    185 [P6_{3}cm] 000l c′− 000l GS [hh2{\bar h}l] G    
    [6_{3}] [c + 6_{3}] c
    186 [P6_{3}mc] 000l GS 000l c′−     [h{\bar h}0l] G
    [c + 6_{3}] [6_{3}] c
    188 [P{\bar 6}c2]     000l G [hh2{\bar h}l] G    
    c c
    190 [P{\bar 6}c2] 000l G         [h{\bar h}0l] G
    c c
    192 [P6/mcc] 000l c′− 000l c′− [hh2{\bar h}l] G [h{\bar h}0l] G
    c c c c
    193 [P6_{3}/mcm] 00l c′− 000l GS [hh2{\bar h}l] G    
    [6_{3}] [c + 6_{3}] c
    194 [P6_{3}/mmc] 000l GS 000l c′−     [h{\bar h}0l] G
    [c + 6_{3}] [6_{3}] c

    Point groups 23, m3, 432, m3m

    Space groupIncident-beam direction
    [100][110][uv0][uuw]
    (cyclic)(cyclic)(cyclic)(cyclic)
    198 P [2_{1}]3 00l, 0k0 S 00l S 00l S    
    [2_{1}] [2_{1}] [2_{1}]
    201 [Pn\bar{3}] 00l, 0k0 n′−     [{\bar k}h0] G    
    [P2/n{\bar 3}] n n
    203 Pd [{\bar 3}] 00l, 0k0 d′−     [{\bar k}h0] G    
    [F2/d{\bar 3}] d d
    205 Pa [{\bar 3}] 00l GS 00l S 00l S    
    [c + 2_{1}] [2_{1}] [2_{1}]
    [P2_{1}/a{\bar 3}] 0k0 b′− [{\bar h}h0] G [{\bar k}h0] G
    [2_{1}] a a
    206 Ia [{\bar 3}]     [{\bar h}h0] G [{\bar k}h0] G    
    [I2_{1}/a{\bar 3}] a a
    212 [P4_{3}32]         00l S    
    [4_{3}]
    213 [P4_{1}32]         00l S    
    [4_{1}]
    218 [P{\bar 4}3n]     00l n hhl G    
    c c
    219 [F{\bar 4}3c]         hhl G    
    c
    220 [I{\bar 4}3d] 0kk G 00l d hhl G    
    [0{\bar k}k]    
    d d d
    222 [Pn{\bar 3}n] 00l, 0k0 n′− 00l n hk0 G hhl G
    n c n c
    223 [Pm{\bar 3}n]     00l n′−     hhl G
    c c
    224 [Pn{\bar 3}m] 00l, 0k0 n′−     hk0 G    
    n n
    226 [Fm{\bar 3}c]             hhl G
    c
    227 [Fd{\bar 3}m] 00l, 0k0 d′−     hk0 G    
    d d
    228 [Fd{\bar 3}c] 00l, 0k0 d′−     hk0 G hhl G
    d d c
    230 [Ia{\bar 3}d] 0kk b′− 00l, [{\bar h}h0] d′− hk0 G hhl G
    [0{\bar k}k]      
    d d a a d
    Conditions in this column are cyclic on h and k.
  • Table 2.5.3.5[link]. This lists conditions for observation of GS bands for the 137 space groups exhibiting these extinctions. These are entered as `G', `S', or `GS', indicating whether a glide plane, screw axis, or both is responsible for the GS band. All three possibilities will lead to a glide line (and hence to both extinction bands) in projection, and one of the procedures (a), (b), or (c) of Section 2.5.3.3[link](iv)[link] above is needed to complete the three-dimensional interpretation. In addition, the presence of horizontal glide planes, which result in systematic absences in these particular cases in the zero-layer pattern, is indicated by the symbol `−'. Where these occur at the site of prospective `G' or `S' bands from other glide or screw elements the symbol of that element is given and the `G' or `S' symbol is omitted.

    The following paragraphs give information on the real-space interpretation of GS band formation, and their specific extinction rules, considered useful in structural interpretation.

    Real-space interpretation of extinction conditions . Dynamic extinctions (GS bands) are essentially a property of symmetry in reciprocal space. However, since diagrams from IT I and A are used there is a need to give an equivalent real-space description. These bands are associated with the half-unit-cell-translational glide planes and screw axes represented in these diagrams. Inconsistencies between `conventional' and `physical' real-space descriptions, however, become more apparent in dynamical electron diffraction, which is dependent upon three-dimensional scattering physics, than in X-ray diffraction. Also, the distinction between general (symmorphic) and specific (non-symmorphic) extinctions is more basic (in the former case). This is clarified by the following points:

    • (i) Bravais lattice centring restricts the conditions for observation of GS bands. For example, in space group Abm2 (No. 39), `A' centring prevents observation of the GS bands associated with the `b' glide at the [001] zone-axis orientation; this observation, and hence verification of the b glide, must be made at the lower-symmetry zone axes [0vw] (see Table 2.5.3.5)[link]. In the exceptional cases of space groups [I2_{1}2_{1}2_{1}] and [I2_{1}3] (Nos. 24 and 199), conditions for the observation of the relevant GS bands are completely prevented by body centring; here the screw axes of the symmorphic groups I222 and I23 are parallel to the screw axes of their non-symmorphic derivatives. However, electron crystallographic methods also include direct structure imaging by HREM, and it is important to note here that while the indistinguishability encountered in data sets acquired in Fourier space applies to both X-ray diffraction and CBED (notwithstanding possible differences in HOLZ symmetries), this limitation does not apply to the HREM images (produced by dynamic scattering) yielding an approximate structure image for the (zone-axis) projection. This technique then becomes a powerful tool in space-group research by supplying phase information in a different form.

    • (ii) A different complication, relating to nomenclature, occurs in the space groups [P\bar{4}3n], Pn3n and Pm3n (Nos. 218, 222 and 223) where `c' glides parallel to a diagonal plane of the unit cell occur as primary non-symmorphic elements (responsible for reciprocal-space extinctions) but are not used in the Hermann–Maugin symbol; instead the derivative `n' glide planes are used as characters, resulting in an apparent lack of correspondence between the conventionally given real-space symbols and the reciprocal-space extinctions.

      (Note: In IT I non-symmorphic reflection rules which duplicate rules given by lattice centring, or those which are a consequence of more general rules, are given in parentheses; in IT A this clarification by parenthesizing, helpful for electron-diffraction analysis, has been removed.)

    • (iii) Finally, diamond glides (symbol `d') require special consideration since they are associated with translations [{1 \over 4}], [{1 \over 4}], [{1 \over 4}], and so would appear not to qualify for GS bands; however, this translation is a result of the conventional cell being defined in real rather than reciprocal space where the extinction symmetry is formed. Hence `d' glides occur only in F-centred lattices (most obviously Nos. 43, 70, 203, 277 and 228). These have correspondingly an I-centred reciprocal lattice for which the zero-layer two-dimensional unit cell has an edge of [a^{*\prime\prime} = 2a^{*}]. Consequently, the first-order row reflection along the diamond glide retains the reciprocal-space anti-symmetry on the basis of this physical unit cell (halved in real space), and leads to the labelling of odd-order reflections as [4n + 2] (instead of [2n + 1] when the cell is not halved). Additionally, although seven space groups are I-centred in real space with the conventional unit cell (Nos. 109, 110, 122, 141, 142, 220 and 230), these space groups are F-centred with the transformation [a'' = [110]], [b'' = [1\bar{1}0]], and correspondingly I-centred in the reciprocal-space cell as before, but the directions [100], [010] and reflection rows h00, 0k0 become replaced by directions [110] (or [[1\bar{1}0]]) and rows hh0, [h\bar{h}0], in the entries of Table 2.5.3.5[link].

    Extinction rules for symmetry elements appearing in Table 2.5.3.5[link]. Reflection indices permitting observation of G and S bands follow [here `zero-layer' and `out-of-zone' (i.e. HOLZ or alternative zone) serve to emphasize that these are zone-axis observations].

    • (i) Vertical glide planes lead to `G' bands in reflections as listed (`a', `b', `c' and `n' glides):

      h0l, hk0, 0kl out-of-zone reflections (for glide planes having normals [010], [001] and [100]) having [h + l], [h + k], [k + l = 2n + 1], respectively, in the case of `n' glides, or h, k, l odd in the case of `a', `b' or `c' glides, respectively;

      h00, 0k0, 00l zero-layer reflections with h, k or l odd.

      Correspondingly for `d' glides:

      • (a) In F-centred cells:

        h0l, hk0, 0kl out-of-zone reflections (for glide planes having normals [010], [100] and [001], having [h + l], [k + l], or [h + k = 4n + 2], respectively, with h, k and l even; and (space group No. 43 only) zero-layer reflections h00, 0k0 with h, k even and [= 4n + 2].

      • (b) In I-centred cells:

        hhl (cyclic on h, k, l for cubic groups) out-of-zone reflections having [2h + l = 4n + 2], with l even; and zero-layer reflections hh0, [h\bar{h}0] (cyclic on h, k, l for cubic groups) having h odd.

    • (ii) Horizontal screw axes, namely [2_{1}] or the [2_{1}] component of screw axes [4_{1}], [4_{3}], [6_{1}], [6_{3}], [6_{5}], lead to `S' bands in reflection rows parallel to the screw axis, i.e. either h00, 0k0 or 00l, with conventional indexing, for h, k or l odd.

    • (iii) Horizontal glide planes lead to zero-layer absences rather than GS bands. When these prevent observation of a specific GS band (by removing the two-dimensional conditions), the symbol `−' indicates a situation where, in general, there will simply be absences for the odd-order reflections. However, Ishizuka & Taftø (1982)[link] were the first to observe finite-intensity narrow bands under these conditions, and it is now appreciated that with a sufficient crystal thickness and a certain minimum for the z-axis repeat distance, GS bands can be recorded by violating the condition for horizontal-mirror-plane (m′) extinction while satisfying the condition for G or S, achieved by appropriate tilts away from the exact zone-axis orientation [see Section 2.5.3.3[link](iv)[link]].

2.5.3.5. Space-group analyses of single crystals; experimental procedure and published examples

| top | pdf |

2.5.3.5.1. Stages of procedure

| top | pdf |

  • (i) Zone-axis patterns . The first need is to record a principal zone-axis pattern. From this, the rotational order X of the vertical axis and associated mirror (including glide-line) components are readily observed (see all examples).

    This pattern may include part of the higher-order Laue zone; in particular the closest or first-order Laue zone (FOLZ) should be included in order to establish the presence or absence of horizontal glide planes, as illustrated in Fig. 2.5.3.3[link]. The projection approximation frequently applies to the zone-axis pattern, particularly when this is obtained from thin crystals (although this cannot apply by definition to the FOLZ). This is indicated by the absence of fine-line detail in the central beam particularly; identification of the projected symmetry is then straightforward.

  • (ii) Laue circle patterns . Next, it is usual to seek patterns in which discs around the Laue circle include the line [m_{R}] (Fig. 2.5.3.2[link]). The internal disc symmetries observed together with those from the zone-axis pattern will determine a diffraction group, classifying the zero-layer symmetry. [Fig. 6(c) of Goodman & Whitfield (1980)[link] gives an example of Laue-circle symmetries.]

  • (iii) Alternative zone axes or higher-order Laue zones . Finally, alternative zone or higher-order Laue-zone patterns may be sought for additional three-dimensional data: (a) to determine the three-dimensional extinction rules, (b) to test for centrosymmetry, or (c) to test for the existence of mirror planes perpendicular to the principal rotation axis. These procedures are illustrated in the following examples.

2.5.3.5.2. Examples

| top | pdf |

  • (1) Determination of centrosymmetry; examples from the hexagonal system. Fig. 2.5.3.4[link](a) illustrates the allocation of planar point groups from [0001] zone-axis patterns of [\beta \hbox{-Si}_{3}\hbox{N}_{4}] (left-hand side) and β-GaS (right-hand side); the patterns exhibit point symmetries of 6 and 6mm, respectively, as indicated by the accompanying geometric figures, permitting point groups 6 or [6/m], and 6mm or [6/mmm], in three dimensions. Alternative zone axes are required to distinguish these possibilities, the actual test used (testing for the element m′ or the centre of symmetry) being largely determined in practice by the type of crystal preparation.

    [Figure 2.5.3.4]

    Figure 2.5.3.4 | top | pdf |

    (a) Zone-axis patterns from hexagonal structures β-[\hbox{Si}_{3}\hbox{N}_{4}] (left) and β-GaS (right) together with the appropriate planar figures for point symmetries 6 and 6mm, respectively. (b) [[12\bar{1}0]] zone-axis pattern from β-[\hbox{Si}_{3}\hbox{N}_{4}], showing Friedel's law breakdown in symmetry between 0002 and [000\bar{2}] reflections (Bando, 1981[link]). (c) Conjugate pair of [1\bar{1}01/\bar{1}101] patterns from β-GaS, taken near the [[110\bar{2}]] zone axis, showing a translational symmetry associated with structural centrosymmetry.

    Fig. 2.5.3.4[link](b) shows the CBED pattern from the [[11\bar{2}0]] zone axis of [\beta \hbox{-Si}_{3}\hbox{N}_{4}] (Bando, 1981[link]), using a crystal with the corresponding cleavage faces. The breakdown of Friedel's law between reflections 0002 and [000\bar{2}] rules out the point group [6/m] (the element m′ from the first setting is not present) and establishes 6 as the correct point group.

    Also, the GS bands in the 0001 and [000\bar{1}] reflections are consistent with the space group [P6_{3}]. [Note: screw axes [6_{1}], [6_{3}] and [6_{5}] are not distinguished from these data alone (Tanaka et al., 1983[link]).]

    Fig. 2.5.3.4[link](c) shows CBED patterns from the vicinity of the [[1\bar{1}02]] zone axis of β-GaS, only 11.2° rotated from the [0001] axis and accessible using the same crystal as for the previous [0001] pattern. This shows a positive test for centrosymmetry using a conjugate reflection pair [1\bar{1}01/\bar{1}10\bar{1}], and establishes the centrosymmetric point group [6/mmm], with possible space groups Nos. 191, 192, 193 and 194. Rotation of the crystal to test the extinction rule for [hh2\bar{h}l] reflections with l odd (Goodman & Whitfield, 1980[link]) establishes No. 194 [(P6_{3}/mmc)] as the space group.

    Comment: These examples show two different methods for testing for centrosymmetry. The ±H test places certain requirements on the specimen, namely that it be reasonably accurately parallel-sided – a condition usually met by easy-cleavage materials like GaS, though not necessarily by the wedge-shaped refractory Si3N4 crystals. On the other hand, the 90° setting, required for direct observation of a possible perpendicular mirror plane, is readily available in these fractured samples, but not for the natural cleavage samples.

  • (2) Point-group determination in the cubic system, using Table 2.5.3.3.[link] Fig. 2.5.3.5[link] shows [001] (cyclic) zone-axis patterns from two cubic materials, which serve to illustrate the ability to distinguish cubic point groups from single zone-axis patterns displaying detailed central-beam structures. The left-hand pattern, from the mineral gahnite (Ishizuka & Taftø, 1982[link]) has 4mm symmetry in both the whole pattern and the central (bright-field) beam, permitting only the BESR group [4mm1_{R}] for the cubic system (column III, Table 2.5.3.3)[link]; this same observation establishes the crystallographic point group as m3m (column V of Table 2.5.3.3[link]). The corresponding pattern for the χ-phase precipitate of stainless steel (Steeds & Evans, 1980[link]) has a whole-pattern symmetry of only 2mm, lower than the central-beam (bright-field) symmetry of 4mm (this lower symmetry is made clearest from the innermost reflections bordering the central beam). This combination leads to the BESR group [4_{R}mm_{R}] (column III, Table 2.5.3.3)[link], and identifies the cubic point group as [\bar{4}3m].

    [Figure 2.5.3.5]

    Figure 2.5.3.5 | top | pdf |

    Zone-axis patterns from cubic structures gahnite (left) (Ishizuka & Taftø, 1982[link]) and χ-phase precipitate (right) (Steeds & Evans, 1980[link]).

  • (3) Analysis of data from FeS2 illustrating use of Tables 2.5.3.4[link] and 2.5.3.5[link]. FeS2 has a cubic structure for which a complete set of data has been obtained by Tanaka et al. (1983)[link]; the quality of the data makes it a textbook example (Tanaka & Terauchi, 1985[link]) for demonstrating the interpretation of extinction bands.

    Figs. 2.5.3.6[link](a) and (b) show the [001] (cyclic) exact zone-axis pattern and the pattern with symmetrical excitation of the 100 reflection, respectively (Tanaka et al., 1983[link]).

    [Figure 2.5.3.6]

    Figure 2.5.3.6 | top | pdf |

    (a) CBED pattern from the exact [001] (cyclic) zone-axis orientation of FeS2. (b) Pattern from the [001] zone axis oriented for symmetrical excitation of the 100 reflection (central in the printed pattern) [from the collection of patterns presented in Tanaka et al. (1983)[link]; originals kindly supplied by M. Tanaka].

    • (i) Using Table 2.5.3.4[link], since there are GS bands, the pattern group must be listed in column II(ii); since a horizontal `b' glide plane is present (odd rows are absent in the [b^{*}] direction), the symbol must contain a `b′' (or `a′') (cf. Fig. 2.5.3.3[link]). The only possible cubic group from Table 2.5.3.4[link] is No. 205.

    • (ii) Again, a complete GS cross, with both G and S arms, is present in the 100 reflection (central in Fig. 2.5.3.6b[link]), confirmed by mirror symmetries across the G and S lines. From Table 2.5.3.5[link] only space group No. 205 has the corresponding entry in the column for `[100] cyclic' with GS in the cubic system (space groups Nos. 198–230). Additional patterns for the [110] setting, appearing in the original paper (Tanaka et al., 1983[link]), confirm the cubic system, and also give additional extinction characteristics for 001 and 1[\bar{1}]0 reflections (Tanaka et al., 1983[link]; Tanaka & Terauchi, 1985[link]).

  • (4) Determination of centrosymmetry and space group from extinction characteristics. Especially in working with thin crystals used in conjunction with high-resolution lattice imaging, it is sometimes most practical to determine the point group (i.e. space-group class) from the dynamic extinction data. This is exemplified in the Moodie & Whitfield (1984)[link] studies of orthorhombic materials. Observations on the zero-layer pattern for [\hbox{Ge}_{3}\hbox{SbSe}_{3}] with a point symmetry of 2mm, and with GS extinction bands along odd-order h00 reflections, together with missing reflection rows in the 0k0 direction, permit identification from Table 2.5.3.4[link]. This zone-axis pattern has the characteristics illustrated in Figs. 2.5.3.3[link] and hence (having both missing rows and GS bands) should be listed in both II(ii) and II(iii). Hence the diffraction group must be either No. 40 or 41. Here, the class mmm, and hence centrosymmetry, has been identified through non-symmorphic elements.

    This identification leaves seven possible space groups, Nos. 52, 54, 56, 57, 60, 61 and 62, to be distinguished by hkl extinctions.

    The same groups are identified from Table 2.5.3.5[link] by seeking the entry GS `−' in one of the [001] (cyclic) entries for the orthorhombic systems. With the assumption that no other principal zone axis is readily available from the same sample (which will generally be true), Table 2.5.3.5[link], in the last three columns, indicates which minor zone axes should be sought in order to identify the space group, from the glide-plane extinctions of `G' bands. For example, space group 62 has no h0l extinctions, but will give 0kl extinction bands `G' according to the rules for an `n' glide, i.e. in reflections for which [k + l = 2n + 1]. Again, if the alternative principal settings are available (from the alternative cleavages of the sample) the correct space group can be found from the first three columns of Table 2.5.3.5[link].

    From the above discussions it will be clear that Tables 2.5.3.4[link] and 2.5.3.5[link] present information in a complementary way: in Table 2.5.3.4[link] the specific pattern group is indexed first with the possible space groups following, while in Table 2.5.3.5[link] the space group is indexed first, and the possible pattern symmetries are then given, in terms of the standard International Tables setting.

2.5.3.6. Use of CBED in study of crystal defects, twins and non-classical crystallography

| top | pdf |

  • (i) Certain crystal defects lend themselves to analysis by CBED and LACBED. In earlier work, use was made of the high sensitivity of HOLZ line geometry to unit-cell parameters (Jones et al., 1977[link]). A computer program (Tanaka & Terauchi, 1985[link]) is available for simulating relative line positions from lattice geometry, assuming kinematical scattering, which at least provides a valid starting point since these spacings are mainly determined from geometric considerations. Fraser et al. (1985)[link], for example, obtained a sensitivity of 0.03% in measurements of cubic-to-tetragonal distortions in this way, although the absolute accuracy was not established.

  • (ii) By contrast, techniques have been devised for evaluating Bragg-line splitting caused by the action of a strain field within the single crystal. One method depends upon the observation of splitting in HOLZ lines (Carpenter & Spence, 1982[link]). More recently, the use of LACBED has allowed quantitative evaluation of lattice distortions in semiconductor heterostructures (e.g. containing GaAs–InGaAs interfaces). This technique has been reviewed by Chou et al. (1994)[link].

  • (iii) Quite distinct from this is the analysis of stacking faults between undistorted crystal domains (Johnson, 1972[link]). Coherent twin boundaries with at least a two-dimensional coincidence site lattice can be considered in a similar fashion (Schapink et al., 1983[link]). In marked contrast to electron-microscopy image analysis these boundaries need to be parallel (or nearly so) to the crystal surfaces rather than inclined or perpendicular to them for analysis by CBED or LACBED.

    The term `rigid-body displacement' (RBD) is used when it is assumed that no strain field develops at the boundary. A classification of the corresponding bi-crystal symmetries was developed by Schapink et al. (1983)[link] for these cases. Since experimental characterization of grain boundaries is of interest in metallurgy, this represents a new area for the application of LACBED and algorithms invoking reciprocity now make routine N-beam analysis feasible.

    The original investigations, of a mid-plane stacking fault in graphite (Johnson, 1972[link]) and of a mid-plane twin boundary in gold (Schapink et al., 1983[link]), represent classic examples of the influence of bi-crystal symmetry on CBED zone-axis patterns, whereby the changed central-plane symmetry is transformed through reciprocity into an exact diffraction symmetry. (a) In the graphite [(P6_{3}/mmc)] example, the hexagonal pattern of the unfaulted graphite is replaced by a trigonal pattern with mid-plane faulting. Here a mirror plane at the centre of the perfect crystal (A–B–A stacking) is replaced by an inversion centre at the midpoint of the single rhombohedral cell A–B–C; the projected symmetry is also reduced from hexagonal to trigonal: both whole pattern and central beam then have the symmetry of 3m1. The 2H polytype of TaS2 [(P6_{3}/mmc)] (Tanaka & Terauchi, 1985[link]) gives a second clear example. (b) In the case of a [111] gold crystal, sectioning the f.c.c. structure parallel to [111] preparatory to producing the twin already reduces the finite crystal symmetry to [R\bar{3}m], i.e. a trigonal space group for which the central beam, and the HOLZ reflections in particular, exhibit the trigonal symmetry of 31m (rather than the 3m1 of trigonal graphite). A central-plane twin boundary with no associated translation introduces a central horizontal mirror plane into the crystal. For the zone-axis pattern the only symmetry change will be in the central beam, which will become centrosymmetric, increasing its symmetry to 6mm. Using diffraction-group terminology these cases are seen to be relative inverses. Unfaulted graphite has the BESR group [6mm1_{R}] (central beam and whole pattern hexagonal); central-plane faulting results in a change to the group [6_{R}mm_{R}]. Unfaulted [111] gold correspondingly has the BESR group symmetry [6_{R}mm_{R}]; central-plane twinning results in the addition of the element [1_{R}] (for a central mirror plane), leading to the group [6mm1_{R}].

  • (iv) Finally, no present-day discussion of electron-crystallographic investigations of symmetry could be complete without reference to two aspects of non-classical symmetries widely discussed in the literature in recent years. The recent discovery of noncrystallographic point symmetries in certain alloys (Shechtman et al., 1984[link]) has led to the study of quasi-crystallinity. An excellent record of the experimental side of this subject may be found in the book Convergent-beam electron diffraction III by Tanaka et al. (1994)[link], while the appropriate space-group theory has been developed by Mermin (1992)[link]. It would be inappropriate to comment further on this new subject here other than to state that this is clearly an area of study where combined HREM, CBED and selected-area diffraction (SAD) evidence is vital to structural elucidation.

    The other relatively new topic is that of modulated structures. From experimental evidence, two distinct structural phenomena can be distinguished for structures exhibiting incommensurate superlattice reflections. Firstly, there are `Vernier' phases, which exist within certain composition ranges of solid solutions and are composed of two extensive substructures, for which the superspace-group nomenclature developed by de Wolff et al. (1981)[link] is structurally valid (e.g. Withers et al., 1993[link]). Secondly, there are structures essentially composed of random mixtures of two or more substructures existing as microdomains within the whole crystal (e.g. Grzinic, 1985[link]). Here the SAD patterns will contain superlattice reflections with characteristic profiles and/or irregularities of spacings. A well illustrated review of incommensurate-structure analysis in general is given in the book by Tanaka et al. (1994)[link], while specific discussions of this topic are given by Goodman et al. (1992)[link], and Goodman & Miller (1993)[link].

2.5.3.7. Present limitations and general conclusions

| top | pdf |

The list of examples given here must necessarily be regarded as unsatisfactory considering the vastness of the subject, although some attempt has been made to choose a diverse range of problems which will illustrate the principles involved. Some particular aspects, however, need further mention.

One of these concerns the problem of examining large-unit-cell materials with a high diffraction-pattern density. This limits the possible convergence angle, if overlap is to be avoided, and leaves numerous but featureless discs [for example Goodman (1984b)[link]]. Technical advances which have been made to overcome this problem include the beam-rocking technique (Eades, 1980[link]) and LACBED (Tanaka et al., 1980[link]), both of which are reviewed by Tanaka & Terauchi (1985)[link] and Eades et al. (1983)[link]. The disadvantage of these latter methods is that they both require a significantly larger area of specimen than does the conventional technique, and it may be that more sophisticated methods of handling the crowded conventional patterns are still needed.

Next, the matter of accuracy must be considered. There are two aspects of the subject where this is of concern. Firstly, there is a very definite limit to the sensitivity with which symmetry can be detected. In a simple structure of medium-light atoms, displacements of say 0.1 Å or less from a pseudomirror plane could easily be overlooked. An important aspect of CBED analysis, not mentioned above, is the N-beam computation of patterns which is required when something approaching a refinement (in the context of electron diffraction) is being attempted. Although this quantitative aspect has a long history [for example see Johnson (1972)[link]], it has only recently been incorporated into symmetry studies as a routine (Creek & Spargo, 1985[link]; Tanaka, 1994[link]). Multi-slice programs which have been developed to produce computer-simulated pattern output are available (Section 2.5.3.8[link]).

Next there is concern as to the allocation of a space group to structures which microscopically have a much lower symmetry (Goodman et al., 1984[link]). This arises because the volume sampled by the electron probe necessarily contains a large number of unit cells. Reliable microscopic interpretation of certain nonstoichiometric materials requires that investigations be accompanied by high-resolution microscopy. Frequently (especially in mineralogical samples), nonstoichiometry implies that a space group exists only on average, and that the concept of absolute symmetry elements is inapplicable.

From earlier and concluding remarks it will be clear that combined X-ray/CBED and CBED/electron-microscopy studies of inorganic materials represents the standard ideal approach to space-group analysis at present; given this approach, all the space-group problems of classical crystallography appear soluble. As has been noted earlier, it is important that HREM be considered jointly with CBED in determining space group by electron crystallography, and that only by this joint study can the so-called `phase problem' be completely overcome. The example of the space-group pairs [I222/I2_{1}2_{1}2_{1}] and [I23/I2_{1}3] has already been cited. Using CBED, it might be expected that FOLZ lines would show a break from twofold symmetry with the incident beam aligned with a [2_{1}] axis. However, a direct distinction should be made apparent from high-resolution electron micrographs. Other less clear-cut cases occur where the HREM images allow a space-group distinction to be made between possible space groups of the same arithmetic class, especially when only one morphology is readily obtained (e.g. [P222_{1}], [P22_{1}2_{1}], [P2_{1}2_{1}2_{1}]).

The slightly more subtle problem of distinguishing enantiomorphic space-group pairs can be solved by one of two approaches: either the crystal must be rotated around an axis by a known amount to obtain two projections, or the required three-dimensional phase information can be deduced from specific three-beam-interaction data. This problem is part of the more general problem of solving handedness in an asymmetric structure, and is discussed in detail by Johnson & Preston (1994)[link].

2.5.3.8. Computer programs available

| top | pdf |

  • (1) A FORTRAN source listing of program TCBED for simulating three-dimensional convergent-beam patterns with absorption by the Bloch-wave method: Zuo et al. (1989)[link] [see also Electron microdiffraction (Spence & Zuo, 1992[link]) for other useful programs and worked examples for the analysis of these diffraction patterns]. Contact J. M. Zuo or J. C. H. Spence, Physics Department, Arizona State University, Tempe, Arizona, USA.

  • (2) A package for CBED pattern simulation by both Bloch-wave and multi-slice methods is available from P. Stadelmann (pierre.stadelmann@cime.uhd.edfl.ch ), Lausanne, Switzerland, in UNIX for workstations [Silicon Graphics, Dec Alpha (OSF), IBM RISC 6000, SUN and HP-9000].

  • (3) HOLZ line simulations: Listing for PC 8801 (NEC): Tanaka & Terauchi (1985[link], pp. 174–175).

2.5.4. Electron-diffraction structure analysis (EDSA)4

| top | pdf |
B. K. Vainshteinc and B. B. Zvyagind

2.5.4.1. Introduction

| top | pdf |

Electron-diffraction structure analysis (EDSA) (Vainshtein, 1964[link]) based on electron diffraction (Pinsker, 1953[link]) is used for the investigation of the atomic structure of matter together with X-ray and neutron diffraction analysis. The peculiarities of EDSA, as compared with X-ray structure analysis, are defined by a strong interaction of electrons with the substance and by a short wavelength λ. According to the Schrödinger equation (see Section 5.2.2[link] ) the electrons are scattered by the electrostatic field of an object. The values of the atomic scattering amplitudes, [f_{e}], are three orders higher than those of X-rays, [f_{x}], and neutrons, [f_{n}]. Therefore, a very small quantity of a substance is sufficient to obtain a diffraction pattern. EDSA is used for the investigation of very thin single-crystal films, of [\sim]5–50 nm polycrystalline and textured films, and of deposits of finely grained materials and surface layers of bulk specimens. The structures of many ionic crystals, crystal hydrates and hydro-oxides, various inorganic, organic, semiconducting and metallo-organic compounds, of various minerals, especially layer silicates, and of biological structures have been investigated by means of EDSA; it has also been used in the study of polymers, amorphous solids and liquids.

Special areas of EDSA application are: determination of unit cells; establishing orientational and other geometrical relationships between related crystalline phases; phase analysis on the basis of [d_{hkl}] and [I_{hkl}] sets; analysis of the distribution of crystallite dimensions in a specimen and inner strains in crystallites as determined from line profiles; investigation of the surface structure of single crystals; structure analysis of crystals, including atomic position determination; precise determination of lattice potential distribution and chemical bonds between atoms; and investigation of crystals of biological origin in combination with electron microscopy (Vainshtein, 1964[link]; Pinsker, 1953[link]; Zvyagin, 1967[link]; Pinsker et al., 1981[link]; Dorset, 1976[link]; Zvyagin et al., 1979[link]).

There are different kinds of electron diffraction (ED) depending on the experimental conditions: high-energy (HEED) (above 30–200 kV), low-energy (LEED) (10–600 V), transmission (THEED), and reflection (RHEED). In electron-diffraction studies use is made of special apparatus – electron-diffraction cameras in which the lens system located between the electron source and the specimen forms the primary electron beam, and the diffracted beams reach the detector without aberration distortions. In this case, high-resolution electron diffraction (HRED) is obtained. ED patterns may also be observed in electron microscopes by a selected-area method (SAD). Other types of electron diffraction are: MBD (microbeam), HDD (high-dispersion), CBD (convergent-beam), SMBD (scanning-beam) and RMBD (rocking-beam) diffraction (see Sections 2.5.2[link] and 2.5.3[link]). The recent development of electron diffractometry, based on direct intensity registration and measurement by scanning the diffraction pattern against a fixed detector (scintillator followed by photomultiplier), presents a new improved level of EDSA which provides higher precision and reliability of structural data (Avilov et al., 1999[link]; Tsipursky & Drits, 1977[link]; Zhukhlistov et al., 1997[link], 1998[link]; Zvyagin et al., 1996[link]).

Electron-diffraction studies of the structure of molecules in vapours and gases is a large special field of research (Vilkov et al., 1978[link]). See also Stereochemical Applications of Gas-Phase Electron Diffraction (1988)[link].

2.5.4.2. The geometry of ED patterns

| top | pdf |

In HEED, the electron wavelength λ is about 0.05 Å or less. The Ewald sphere with radius [\lambda^{-1}] has a very small curvature and is approximated by a plane. The ED patterns are, therefore, considered as plane cross sections of the reciprocal lattice (RL) passing normal to the incident beam through the point 000, to scale [L\lambda] (Fig. 2.5.4.1[link]). The basic formula is [r = |{\bf h}| L\lambda, \hbox{ or } rd = L\lambda, \eqno(2.5.4.1)] where r is the distance from the pattern centre to the reflection, h is the reciprocal-space vector, d is the appropriate interplanar distance and L is the specimen-to-screen distance. The deviation of the Ewald sphere from a plane at distance h from the origin of the coordinates is [\delta_{h} = h^{2} \lambda / 2]. Owing to the small values of λ and to the rapid decrease of [f_{e}] depending on [(\sin \theta)/\lambda], the diffracted beams are concentrated in a small angular interval ([\leq 0.1] rad).

[Figure 2.5.4.1]

Figure 2.5.4.1 | top | pdf |

Ewald spheres in reciprocal space. Dotted line: electrons, solid line: X-rays.

Single-crystal ED patterns image one plane of the RL. They can be obtained from thin ideal crystalline plates, mosaic single-crystal films, or, in the RHEED case, from the faces of bulk single crystals. Point ED patterns can be obtained more easily owing to the following factors: the small size of the crystals (increase in the dimension of RL nodes) and mosaicity – the small spread of crystallite orientations in a specimen (tangential tension of the RL nodes). The crystal system, the parameters of the unit cell and the Laue symmetry are determined from point ED patterns; the probable space group is found from extinctions. Point ED patterns may be used for intensity measurements if the kinematic approximation holds true or if the contributions of the dynamic and secondary scattering are not too large.

The indexing of reflections and the unit-cell determination are carried out according to the formulae relating the RL to the DL (direct lattice) (Vainshtein, 1964[link]; Pinsker, 1953[link]; Zvyagin, 1967[link]).

Under electron-diffraction conditions crystals usually show a tendency to lie down on the substrate plane on the most developed face. Let us take this as (001). The vectors a and b are then parallel, while vector [c^{*}] is normal to this plane, and the RL points are considered as being disposed along direct lines parallel to the axis [c^{*}] with constant hk and variable l.

The interpretation of the point patterns as respective RL planes is quite simple in the case of orthogonal lattices. If the lattice is triclinic or monoclinic the pattern of the crystal in the position with the face (001) normal to the incident beam does not have to contain hk0 reflections with non-zero h and k because, in general, the planes ab and [a^{*}b^{*}] do not coincide. However, the intersection traces of direct lines hk with the plane normal to them (plane ab) always form a net with periods [(a \sin \gamma)^{-1}, (b \sin \gamma)^{-1}, \hbox{and angle } \gamma' = \pi - \gamma \eqno(2.5.4.2a)] (Fig. 2.5.4.2[link]). The points hkl along these directions hk are at distances [\eta = ha^{*} \cos \beta^{*} + kb^{*} \cos \alpha^{*} + lc^{*} \eqno(2.5.4.3)] from the ab plane.

[Figure 2.5.4.2]

Figure 2.5.4.2 | top | pdf |

Triclinic reciprocal lattice. Points: open circles, projection net: black circles.

By changing the crystal orientation it is possible to obtain an image of the [a^{*} b^{*}] plane containing hk0 reflections, or of other RL planes, with the exception of planes making a small angle with the axis [c^{*}].

In the general case of an arbitrary crystal orientation, the pattern is considered as a plane section of the system of directions hk which makes an angle ϕ with the plane ab, intersecting it along a direction [uv]. It is described by two periods along directions 0h, 0k; [(a \sin \gamma \cos \psi_{h})^{-1}, (b \sin \gamma \cos \psi_{k})^{-1}, \eqno(2.5.4.2b)] with an angle γ″ between them satisfying the relation [\cos \gamma'' = \sin \psi_{h} \sin \psi_{k} - \cos \psi_{h} \cos \psi_{k} \cos \gamma, \eqno(2.5.4.2c)] and by a system of parallel directions [p_{h}h + p_{k}k = l\hbox{;} \quad l = 0, \pm 1, \pm 2,\ldots. \eqno(2.5.4.4)] The angles [\psi_{h}, \psi_{k}] are formed by directions 0h, 0k in the plane of the pattern with the plane ab. The coefficients [p_{h}, p_{k}] depend on the unit-cell parameters, angle ϕ and direction [uv]. These relations are used for the indexing of reflections revealed near the integer positions hkl in the pattern and for unit-cell calculations (Vainshtein, 1964[link]; Zvyagin, 1967[link]; Zvyagin et al., 1979[link]).

In RED patterns obtained with an incident beam nearly parallel to the plane ab one can reveal all the RL planes passing through [c^{*}] which become normal to the beam at different azimuthal orientations of the crystal.

With the increase of the thickness of crystals (see below, Chapter 5.1[link] ) the scattering becomes dynamical and Kikuchi lines and bands appear. Kikuchi ED patterns are used for the estimation of the degree of perfection of the structure of the surface layers of single crystals for specimen orientation in HREM (IT C, 2004[link], Section 4.3.8[link] ). Patterns obtained with a convergent beam contain Kossel lines and are used for determining the symmetry of objects under investigation (see Section 5.1.2[link] ).

Texture ED patterns are a widely used kind of ED pattern (Pinsker, 1953[link]; Vainshtein, 1964[link]; Zvyagin, 1967[link]). Textured specimens are prepared by substance precipitation on the substrate, from solutions and suspensions, or from gas phase in vacuum. The microcrystals are found to be oriented with a common (developed) face parallel to the substrate, but they have random azimuthal orientations. Correspondingly, the RL also takes random azimuthal orientations, having [c^{*}] as the common axis, i.e. it is a rotational body of the point RL of a single crystal. Thus, the ED patterns from textures bear a resemblance, from the viewpoint of their geometry, to X-ray rotation patterns, but they are less complicated, since they represent a plane cross section of reciprocal space.

If the crystallites are oriented by the plane (hkl), then the axis [[hkl]^{*}] is the texture axis. For the sake of simplicity, let us assume that the basic plane is the plane (001) containing the axes a and b, so that the texture axis is [[001]^{*}], i.e. the axis [c^{*}]. The matrices of appropriate transformations will define a transition to the general case (see IT A , 2005[link]). The RL directions [hk = \hbox{constant}], parallel to the texture axis, transform to cylindrical surfaces, the points with [\eta_{hkl} = \hbox{constant}] are in planes perpendicular to the texture axis, while any `tilted' lines transform to cones or hyperboloids of rotation. Each point hkl transforms to a ring lying on these surfaces. In practice, owing to a certain spread of [c^{*}] axes of single crystals, the rings are blurred into small band sections of a spherical surface with the centre at the point 000; the oblique cross section of such bands produces reflections in the form of arcs. The main interference curves for texture patterns are ellipses imaging oblique plane cross sections of the cylinders hk (Fig. 2.5.4.3[link]).

[Figure 2.5.4.3]

Figure 2.5.4.3 | top | pdf |

Formation of ellipses on an electron-diffraction pattern from an oblique texture.

At the normal electron-beam incidence (tilting angle [\varphi = 0^{\circ}]) the ED pattern represents a cross section of cylinders perpendicular to the axis [c^{*}], i.e. a system of rings.

On tilting the specimen to an angle ϕ with respect to its normal position (usually [\varphi \simeq 60^{\circ}]) the patterns image an oblique cross section of the cylindrical RL, and are called oblique-texture (OT) ED patterns. The ellipses [(hk = \hbox{constant})] and layer lines [(l = \hbox{constant})] for orthogonal lattices are the main characteristic lines of ED patterns along which the reflections are arranged. The shortcoming of oblique-texture ED patterns is the absence of reflections lying inside the cone formed by rotation of the straight line coming from the point 000 at an angle [(90^{\circ} - \varphi)] around the axis [c^{*}] and, in particular, of reflections 00l. However, at ϕ ≲ 60–70° the set of reflections is usually sufficient for structural determination.

For unit-cell determination and reflection indexing the values d (i.e. [|{\bf h}|]) are used, and the reflection positions defined by the ellipses hk to which they belong and the values η are considered. The periods [a^{*}, b^{*}] are obtained directly from [h_{100}] and [h_{010}] values. The period [c^{*}], if it is normal to the plane [a^{*} b^{*}] ([\gamma^{*}] being arbitrary), is calculated as [c^{*} = \eta/l = (h_{hkl}^{2} - h_{hk0}^{2})^{1/2}/l. \eqno(2.5.4.5a)] For oblique-angled lattices [c^{*} = [(h_{{l_1}+l}^{2} + h_{{l_1}-l}^{2} - 2h_{l}^{2})/2]^{1/2}/l. \eqno(2.5.4.5b)] In the general case of oblique-angled lattices the coaxial cylinders hk have radii [\eqalignno{ b_{hk} &= (1/\sin \gamma) [(h^{2} / a^{2}) + (k^{2} / b^{2}) &\cr &\quad - (2hk \cos \gamma / ab)]^{1/2} &(2.5.4.6)\cr}] and it is always possible to use the measured or calculated values [b_{hk}] in (2.5.4.5a)[link] instead of [h_{hk0}], since [\eta = (h_{hkl}^{2} - b_{hk}^{2})^{1/2}. \eqno(2.5.4.7)] In OT patterns the [b_{hk}] and η values are represented by the lengths of the small axes of the ellipses [B_{hk} = L\lambda b_{hk}] and the distances of the reflections hkl from the line of small axes (equatorial line of the pattern) [D_{hkl} = L\lambda \eta /\sin \varphi = hp + ks + lq. \eqno(2.5.4.8)]

Analysis of the [B_{hk}] values gives a, b, γ, while p, s and q are calculated from the [D_{hkl}] values. It is essential that the components of the normal projections [c_{n}] of the axis c on the plane ab measured in the units of a and b are [\eqalign{ x_{n} &= (c / a) (\cos \beta - \cos \alpha \cos \gamma)/\sin^{2} \gamma\cr &= - p / q,\cr y_{n} &= (c / b) (\cos \alpha - \cos \beta \cos \gamma)/\sin^{2} \gamma\cr &= - s / q.\cr} \eqno(2.5.4.9)] Obtaining [x_{n}, y_{n}] one can calculate [c_{n} = [(x_{n} a)^{2} + (y_{n} b)^{2} + 2x_{n} y_{n} ab \cos \gamma]^{1/2}. ] Since [\eqalign{ d_{001} &= L\lambda / q \sin \varphi,\cr c &= (c_{n}^{2} + d_{001}^{2})^{1/2}.\cr} \eqno(2.5.4.10)]

The α, β values are then defined by the relations [\eqalign{ \cos \alpha &= (x_{n} a \cos \gamma + y_{n} b)/c,\cr \cos \beta &= (x_{n} a + y_{n} b \cos \gamma)/c.\cr} \eqno(2.5.4.11)]

Because of the small particle dimensions in textured specimens, the kinematic approximation is more reliable for OT patterns, enabling a more precise calculation of the structure amplitudes from the intensities of reflections.

Polycrystal ED patterns. In this case, the RL is a set of concentric spheres with radii [h_{hkl}]. The ED pattern, like an X-ray powder pattern, is a set of rings with radii [r_{hkl} = h_{hkl} L\lambda. \eqno(2.5.4.12)]

2.5.4.3. Intensities of diffraction beams

| top | pdf |

The intensities of scattering by a crystal are determined by the scattering amplitudes of atoms in the crystal, given by (see also Section 5.2.1[link] ) [\eqalign{ f_{e}^{\rm abs} (s) &= 4\pi K \int\varphi (r) r^{2} {\sin sr \over sr} \ \hbox{d}r\hbox{;}\cr K &= {2\pi me \over h^{2}}\hbox{; }f_{e} = K^{-1} f_{e}^{\rm abs},\cr} \eqno(2.5.4.13)] where [\varphi (r)] is the potential of an atom and [s = 4\pi (\sin \theta)/\lambda]. The absolute values of [f_{e}^{\rm abs}] have the dimensionality of length L. In EDSA it is convenient to use [f_{e}] without K. The dimensionality of [f_{e}] is [potential [L^{3}]]. With the expression of [f_{e}] in V Å3 the value [K^{-1}] in (2.5.4.13)[link] is 47.87 V Å2.

The scattering atomic amplitudes [f_{e} (s)] differ from the respective [f_{x} (s)] X-ray values in the following: while [f_{x} (0) = Z] (electron shell charge), the atomic amplitude at [s = 0] [f_{e} (0) = 4\pi {\textstyle\int} \varphi (r) r^{2} \ \hbox{d}r \eqno(2.5.4.14)] is the `full potential' of the atom. On average, [f_{e} (0) \simeq Z^{1/3}], but for small atomic numbers Z, owing to the peculiarities in the filling of the electron shells, [f_{e} (0)] exhibits within periods of the periodic table of elements `reverse motion', i.e. they decrease with Z increasing (Vainshtein, 1952[link], 1964[link]). At large [(\sin \theta)/\lambda], [f_{e} \simeq Z]. The atomic amplitudes and, consequently, the reflection intensities, are recorded, in practice, up to values of [ (\sin \theta)/\lambda \simeq 0.8\hbox{--}1.2] [\hbox{\AA}^{-1}], i.e. up to [ d_{\min} \simeq 0.4\hbox{--} 0.6\;\hbox{\AA}].

The structure amplitude [\Phi_{hkl}] of a crystal is determined by the Fourier integral of the unit-cell potential (see Chapter 1.2[link] ), [\Phi_{hkl} = {\textstyle\int\limits_{\Omega}} \varphi ({\bf r}) \exp \{2\pi i ({\bf r} \cdot {\bf h})\} \hbox{ d}v_{r}, \eqno(2.5.4.15)] where Ω is the unit-cell volume. The potential of the unit cell can be expressed by the potentials of the atoms of which it is composed: [\varphi ({\bf r}) = {\textstyle\sum\limits_{{\rm cell}, \, i}} \varphi_{{\rm at}\; i} ({\bf r} - {\bf r}_{i}). \eqno(2.5.4.16)] The thermal motion of atoms in a crystal is taken into account by the convolution of the potential of an atom at rest with the probability function [w({\bf r})] describing the thermal motion: [\varphi_{\rm at} = \varphi_{\rm at} ({\bf r}) * w ({\bf r}). \eqno(2.5.4.17)] Accordingly, the atomic temperature factor of the atom in a crystal is [{f_{eT} [(\sin \theta) / \lambda] = f_{e}f_{T} = f_{e} [(\sin \theta) / \lambda] \exp \{- B [(\sin \theta) / \lambda]^{2}\},} \eqno(2.5.4.18)] where the Debye temperature factor is written for the case of isotropic thermal vibrations. Consequently, the structure amplitude is [\Phi_{hkl} = {\textstyle\sum\limits_{{\rm cell}, \, i}} f_{eT_{i}} \exp \{2\pi i (hx_{i} + ky_{i} + lz_{i})\}. \eqno(2.5.4.19)] This general expression is transformed (see IT I, 1952[link]) according to the space group of a given crystal.

To determine the structure amplitudes in EDSA experimentally, one has to use specimens satisfying the kinematic scattering condition, i.e. those consisting of extremely thin crystallites. The limit of the applicability of the kinematic approximation (Blackman, 1939[link]; Vainshtein, 1964[link]) can be estimated from the formula [A = \lambda \left|{\langle \Phi_{{\bf h}} \rangle \over \Omega}\right| t {{\lower7pt\hbox{$\lt$}}\atop{\raise2pt\hbox{$\sim$}}} 1, \eqno(2.5.4.20)] where [\langle \Phi_{{\bf h}} \rangle] is the averaged absolute value of [\Phi_{{\bf h}}] (see also Section 5.2.1[link] ). Since [\langle \Phi_{{\bf h}} \rangle] are proportional to [Z^{0.8}], condition (2.5.4.20)[link] is better fulfilled for crystals with light and medium atoms. Condition (2.5.4.20)[link] is usually satisfied for textured and polycrystalline specimens. But for mosaic single crystals as well, the kinematic approximation limit is, in view of their real structure, substantially wider than estimated by (2.5.4.20)[link] for ideal crystals. The fulfillment of the kinematic law for scattering can be, to a greater or lesser extent, estimated by comparing the decrease of experimental intensity [I_{h} [(\sin \theta)/\lambda]] averaged over definite angular intervals, and sums [{\textstyle\sum}\; f_{\rm obs}^{2} [(\sin \theta)/\lambda]] calculated for the same angular intervals.

For mosaic single-crystal films the integral intensity of reflection is [I_{{\bf h}} = j_{0} S\lambda^{2} \left|{\Phi_{{\bf h}} \over \Omega}\right|^{2} {td_{{\bf h}} \over \alpha} \simeq \Phi_{{\bf h}}^{2} d_{{\bf h}}\hbox{;} \eqno(2.5.4.21)] for textures [I_{{\bf h}} = j_{0} S\lambda^{2} \left|{\Phi_{{\bf h}} \over \Omega}\right|^{2} {tL\lambda p \over 2\pi R' \sin \varphi} \simeq \Phi_{{\bf h}}^{2} p / R'. \eqno(2.5.4.22)] Here [j_{0}] is the incident electron-beam density, S is the irradiated specimen area, t is the thickness of the specimen, α is the average angular spread of mosaic blocks, R′ is the horizontal coordinate of the reflection in the diffraction pattern and p is the multiplicity factor. In the case of polycrystalline specimens the local intensity in the maximum of the ring reflection [I_{{\bf h}} = j_{0} S\lambda^{2} \left|{\Phi_{{\bf h}} \over \Omega}\right|^{2} {td_{{\bf h}}^{2} p \Delta S \over 4\pi L\lambda} \simeq \Phi_{{\bf h}}^{2} d_{{\bf h}}^{2} p \eqno(2.5.4.23)] is measured, where ΔS is the measured area of the ring.

The transition from kinematic to dynamic scattering occurs at critical thicknesses of crystals when [A \geq 1] (2.5.4.20)[link]. Mosaic or polycrystalline specimens then result in an uneven contribution of various crystallites to the intensity of the reflections. It is possible to introduce corrections to the experimental structure amplitudes of the first strong reflections most influenced by dynamic scattering by applying in simple cases the two-wave approximation (Blackman, 1939[link]) or by taking into account multibeam theories (Fujimoto, 1959[link]; Cowley, 1981[link]; Avilov et al. 1984[link]; see also Chapter 5.2[link] ).

The application of kinematic scattering formulae to specimens of thin crystals (5–20 nm) or dynamic corrections to thicker specimens (20–50 nm) permits one to obtain reliability factors between the calculated [\Phi^{\rm calc}] and observed [\Phi^{\rm obs}] structure amplitudes of [ R = 5\hbox{--} 15\%], which is sufficient for structural determinations.

With the use of electron diffractometry techniques, reliability factors as small as [R =] 2–3% have been reached and more detailed data on the distribution of the inner-crystalline potential field have been obtained, characterizing the state and bonds of atoms, including hydrogen (Zhukhlistov et al., 1997[link], 1998[link]; Avilov et al., 1999[link]).

The applicability of kinematics formulae becomes poorer in the case of structures with many heavy atoms for which the atomic amplitudes also contain an imaginary component (Shoemaker & Glauber, 1952[link]). The experimental intensity measurement is made by a photo method or by direct recording (Avilov, 1979[link]). In some cases the amplitudes [\Phi_{hkl}] can be determined from dynamic scattering patterns – the bands of equal thickness from a wedge-shaped crystal (Cowley, 1981[link]), or from rocking curves.

2.5.4.4. Structure analysis

| top | pdf |

The unit cell is defined on the basis of the geometric theory of electron-diffraction patterns, and the space group from extinctions. It is also possible to use the method of converging beams (Section 5.2.2[link] ). The structural determination is based on experimental sets of values [|\Phi_{hkl}|^{2}] or [|\Phi_{hkl}|] (Vainshtein, 1964[link]).

The trial-and-error method may be used for the simplest structures. The main method of determination is the construction of the Patterson functions [P(xyz) = {1 \over \Omega} \left[\Phi_{000}^{2} + 2 \sum\limits_{hkl = -\infty}^{hkl = +\infty} |\Phi_{hkl}|^{2} \cos 2\pi (hx + ky + lz)\right] \eqno(2.5.4.24)] and their analysis on the basis of heavy-atom methods, superposition methods and so on (see Chapter 2.3[link] ). Direct methods are also used (Dorset et al., 1979[link]). Thus the phases of structure factors are calculated and assigned to the observed moduli [\Phi_{\bf h} = |\Phi_{{\bf h}, \, \rm obs}| \exp \{i\alpha_{\rm calc}\}. \eqno(2.5.4.25)]

The distribution of the potential in the unit cell, and, thereby, the arrangement in it of atoms (peaks of the potential) are revealed by the construction of three-dimensional Fourier series of the potential (see also Chapter 1.3[link] ) [\varphi (xyz) = {1 \over \Omega} \sum\limits_{{\bf h}} \Phi_{hkl} \exp \{-2\pi i (hx + ky + lz)\} \eqno(2.5.4.26a)] or projections [\varphi' (xy) = {1 \over S} \sum\limits_{{\bf h}} \Phi_{hk0} \exp \{-2\pi i (hx + ky)\}. \eqno(2.5.4.26b)] The general formulae (2.5.4.26a)[link] and (2.5.4.26b)[link] transform, according to known rules, to the expressions for each space group (see IT I, 1952[link]). If [\Phi_{hkl}] are expressed in V [\hbox{\AA}^{3}] and the volume Ω or the cell area S in [\hbox{\AA}^{3}] and [\hbox{\AA}^{2}], respectively, then the potential ϕ is obtained directly in volts, while the projection of the potential [\varphi'] is in V Å. The amplitudes [|\Phi_{hkl}|] are reduced to an absolute scale either according to a group of strong reflections [{\textstyle\sum} |\Phi_{{\bf h}}|^{\rm calc} = {\textstyle\sum} |\Phi_{{\bf h}}|^{\rm obs} \eqno(2.5.4.27)] or using the Parseval equality [\sum\limits_{{\bf h} = -\infty}^{+\infty} |\Phi_{{\bf h}}|^{2} = \Omega^{2} \langle \varphi^{2}\rangle = \Omega \sum\limits_{i({\rm cell})} {1 \over 2\pi^{2}} \int\limits_{0}^{\infty} f_{eT_{i}}^{2} (s) s^{2} \hbox{ d}s \eqno (2.5.4.28)] or Wilson's statistical method [\langle \Phi^{2} [(\sin \theta) / \lambda]\rangle = {\textstyle\sum\limits_{i}} \;f_{eT_{i}}^{2} [(\sin \theta) / \lambda]. \eqno(2.5.4.29)] The term [\Phi_{000}] defines the mean inner potential of a crystal, and is calculated from [f_{e}(0)] [(2.5.4.13)[link], (2.5.4.19)[link]] [\langle \varphi_{\rm cr}\rangle = \Phi_{000}/\Omega = {1 \over \Omega} \sum f_{e}(0). \eqno(2.5.4.30)] The Fourier series of the potential in EDSA possess some peculiarities (Vainshtein, 1954[link], 1964[link]) which make them different from the electron-density Fourier series in X-ray analysis. Owing to the peculiarities in the behaviour of the atomic amplitudes (2.5.4.13)[link], which decrease more rapidly with increasing [(\sin \theta) / \lambda] compared with [f_{x}], the peaks of the atomic potential [\varphi_{\rm at} (r) = {1 \over 2\pi^{2}} \int f_{eT} (s) {\sin sr \over sr} s^{2} \hbox{ d}s \eqno(2.5.4.31)] are more `blurred' and exhibit a larger half-width than the electron-density peaks [\rho_{\rm at}(r)]. On average, this half-width corresponds to the `resolution' of an electron-diffraction pattern – about 0.5 Å or better. The potential in the maximum (`peak height') does not depend as strongly on the atomic number as in X-ray analysis: [\varphi (0) = {1 \over 2\pi^{2}} \int f_{eT}(s) s^{2} \hbox{ d}s \sim Z^{0.75}, \eqno(2.5.4.32)] while in X-ray diffraction [\rho (0) \sim Z^{1.2}]. In such a way, in EDSA the light atoms are more easily revealed in the presence of heavy atoms than in X-ray diffraction, permitting, in particular, hydrogen atoms to be revealed directly without resorting to difference syntheses as in X-ray diffraction. Typical values of the atomic potential [\varphi (0)] (which depend on thermal motion) in organic crystals are: H ∼ 35, C ∼ 165, O 215 V; in Al crystals 330 V, in Cu crystals 750 V.

The EDSA method may be used for crystal structure determination, depending on the types of electron-diffraction patterns, for crystals containing up to several tens of atoms in the unit cell. The accuracy in determination of atomic coordinates in EDSA is about 0.01–0.005 Å on average. The precision of EDSA makes it possible to determine accurately the potential distribution, to investigate atomic ionization, to obtain values for the potential between the atoms and, thereby, to obtain data on the nature of the chemical bond.

If the positions in the cell are occupied only partly, then the measurement of [\varphi_{i}(0)] gives information on population percentage.

There is a relationship between the nuclear distribution, electron density and the potential as given by the Poisson equation [\nabla^{2} \varphi ({\bf r}) = -4 \pi e [\rho_{+} ({\bf r}) - \rho_{-} ({\bf r})]. \eqno(2.5.4.33)] This makes it possible to interrelate X-ray diffraction, EDSA and neutron-diffraction data. Thus for the atomic amplitudes [f_{e}(s) = 4\pi Ke [Z - f_{x} (s)]s^{-2}, \eqno(2.5.4.34)] where Z is the nuclear charge and [f_{x}] the X-ray atomic scattering amplitude, and for structure amplitudes [\Phi_{hkl} = \pi Ke [Z_{hkl} - F_{hkl}]|{\bf h}|^{-2}, \eqno(2.5.4.35)] where [F_{hkl}] is the X-ray structure amplitude of the electron density of a crystal and [Z_{hkl}] is the amplitude of scattering from charges of nuclei in the cell taking into account their thermal motion. The values [Z_{hkl}] can be calculated easily from neutron-diffraction data, since the charges of the nuclei are known and the experiment gives the parameters of their thermal motion.

In connection with the development of high-resolution electron-microscopy methods (HREM) it has been found possible to combine the data from direct observations with EDSA methods. However, EDSA permits one to determine the atomic positions to a greater accuracy, since practically the whole of reciprocal space with 1.0–0.4 Å resolution is used and the three-dimensional arrangement of atoms is calculated. At the same time, in electron microscopy, owing to the peculiarities of electron optics and the necessity for an objective aperture, the image of the atoms in a crystal [\varphi' ({\bf x}) * A({\bf x})] is a convolution, with the aperture function blurring the image up to 1.5–2 Å resolution. In practice, in TEM one obtains only the images of the heaviest atoms of an object. However, the possibility of obtaining a direct image of a structure with all the defects in the atomic arrangement is the undoubted merit of TEM.

2.5.5. Image reconstruction 5

| top | pdf |
B. K. Vainshteinc

2.5.5.1. Introduction

| top | pdf |

In many fields of physical measurements, instrumental and informative techniques, including electron microscopy and computational or analogue methods for processing and transforming signals from objects investigated, find a wide application in obtaining the most accurate structural data. The signal may be radiation from an object, or radiation transmitted through the object, or reflected by it, which is transformed and recorded by a detector.

The image is the two-dimensional signal [I(xy)] on the observation plane recorded from the whole three-dimensional volume of the object, or from its surface, which provides information on its structure. In an object this information may change owing to transformation of the scattered wave inside an instrument. The real image [J(xy)] is composed of [I(xy)] and noise [N(xy)] from signal disturbances: [J(xy) = I(xy) + N(xy). \eqno(2.5.5.1)]

Image-reconstruction methods are aimed at obtaining the most accurate information on the structure of the object; they are subdivided into two types (Picture Processing and Digital Filtering, 1975[link]; Rozenfeld, 1969[link]):

  • (a) Image restoration – separation of [I(xy)] from the image by means of compensation of distortions introduced in it by an image-forming system as well as by an account of the available quantitative data reflecting its structure.

  • (b) Image enhancement – maximum exclusion from the observed image [J(xy)] (2.5.5.1)[link] of all its imperfections [N(xy)] from both accidental distortions in objects and various `noise' in signals and detector, and obtaining [I(xy)] as the result.

These two methods may be used separately or in combination.

The image should be represented in the form convenient for perception and analysis, e.g. in digital form, in lines of equal density, in points of different density, in half-tones or colour form and using, if necessary, a change or reversal of contrast.

Reconstructed images may be used for the three-dimensional reconstruction of the spatial structure of an object, e.g. of the density distribution in it (see Section 2.5.6[link]).

This section is connected with an application of the methods of image processing in transmission electron microscopy (TEM). In TEM (see Section 2.5.2[link]), the source-emitted electrons are transmitted through an object and, with the aid of a system of lenses, form a two-dimensional image subject to processing.

Another possibility for obtaining information on the structure of an object is structural analysis with the aid of electron diffraction – EDSA. This method makes use of information in reciprocal space – observation and measurement of electron-diffraction patterns and calculation from them of a two-dimensional projection or three-dimensional structure of an object using the Fourier synthesis. To do this, one has to find the relative phases of the scattered beams.

The wavefunction of an electron-microscopic image is written as [\psi_{I} = {#\scr F}^{-1} T{\scr F}{q \psi_{0}}. \eqno(2.5.5.2)] Here [\psi_{0}] is the incident plane wave. When the wave is transmitted through an object, it interacts with the electrostatic potential [\varphi ({\bf r})] [[{\bf r} (xyz)] is the three-dimensional vector in the space of the object]; this process is described by the Schrödinger equation (Section 2.5.2.1[link]). As a result, on the exit surface of an object the wave takes the form [q\psi_{0} ({\bf x})] where q is the transmission function and x is the two-dimensional vector [{\bf x}(xy)]. The diffraction of the wave [q\psi_{0}] is described by the two-dimensional Fourier operator: [ {\scr F}{q} = Q ({\bf u}) = {\textstyle\int} q ({\bf x}) \exp [2 \pi i ({\bf xu})]\ {\rm d}{\bf x.} \eqno(2.5.5.3)]

Here, we assume the initial wave amplitude to be equal to unity and the initial phase to be zero, so that [q\psi_{0} = q], which defines, in this case, the wavefunction in the back focal plane of an objective lens with the reciprocal-space coordinates [{\bf u}(u, v)]. The function Q is modified in reciprocal space by the lens transfer function [T({\bf u})]. The scattered wave transformation into an image is described by the inverse Fourier operator [ {\scr F}^{-1} TQ].

The process of the diffraction [ {\scr F}{q \psi_{0}} = Q], as seen from (2.5.5.1)[link], is the same in both TEM and EDSA. Thus, in TEM under the lens actions [ {\scr F}^{-1} TQ] the image formation from a diffraction pattern takes place with an account of the phases, but these phases are modified by the objective-lens transfer function. In EDSA, on the other hand, there is no distorting action of the transfer function and the `image' is obtained by computing the operation [ {\scr F}^{-1} Q].

The computation of projections, images and Fourier transformation is made by discretization of two-dimensional functions on a two-dimensional network of points – pixels in real space [{\bf x} (x_{j}, y_{k})] and in reciprocal space [{\bf u} (u_{m}, v_{n})].

2.5.5.2. Thin weak phase objects at optimal defocus

| top | pdf |

The intensity distribution [I (xy) \sim |\psi_{I}|^{2}] of an electron wave in the image plane depends not only on the coherent and inelastic scattering, but also on the instrumental functions. The electron wave transmitted through an object interacts with the electrostatic potential [\varphi ({\bf r})] which is produced by the nuclei charges and the electronic shells of the atoms. The scattering and absorption of electrons depend on the structure and thickness of a specimen, and the atomic numbers of the atoms of which it is composed. If an object with the three-dimensional distribution of potential [\varphi ({\bf r})] is sufficiently thin, then the interaction of a plane electron wave [\psi_{0}] with it can be described as the interaction with a two-dimensional distribution of potential projection [\varphi ({\bf x})], [\varphi ({\bf x}) = {\textstyle\int\limits_{0}^{b}} \varphi ({\bf r})\ {\rm d}z, \eqno(2.5.5.4)] where b is the specimen thickness. It should be noted that, unlike the three-dimensional function of potential [\varphi ({\bf r})] with dimension [[M^{1/2} L^{3/2} T^{-1}]], the two-dimensional function of potential projection [\varphi ({\bf x})] has the potential-length dimension [[M^{1/2} L^{1/2} T^{-1}]] which, formally, coincides with the charge dimension. The transmission function, in the general case, has the form [q ({\bf x}) = \exp [-i \sigma \varphi ({\bf x})]] (2.5.2.42)[link], and for weak phase objects the approximation [[\sigma \varphi \ll 1]] [q ({\bf x}) = 1 - i \sigma \varphi ({\bf x}) \eqno(2.5.5.5)] is valid.

In the back focal plane of the objective lens the wave has the form [Q (uv) \cdot T (U) \eqno(2.5.5.6)] [T = A (U) \exp (i \chi U) \eqno(2.5.5.7a)] [\chi (U) = \pi \Delta f \lambda U^{2} + {\pi \over 2} C_{s} \lambda^{3} U^{4}, \eqno(2.5.5.7b)] where [U = (u^{2} + v^{2})^{1/2}\hbox{;} \exp [i \chi (U)]] is the Scherzer phase function (Scherzer, 1949[link]) of an objective lens (Fig. 2.5.5.1[link]), [A(U)] is the aperture function, [C_{s}] the spherical aberration coefficient, and Δf the defocus value [(2.5.2.32)[link] [link] [link]–(2.5.2.35)[link]].

[Figure 2.5.5.1]

Figure 2.5.5.1 | top | pdf |

The χ function and two components of the Scherzer phase function sin [\chi (U)] and cos [\chi (U)].

The bright-field image intensity (in object coordinates) is [I (xy) = |\psi_{I} (xy) * t (xy)|^{2}, \eqno(2.5.5.8)] where [ t = {\scr F}^{-1} [T]]. The phase function (2.5.5.7)[link] depends on defocus, and for a weak phase object (Cowley, 1981[link]) [I (xy) = 1 + 2 \sigma \varphi (xy) * s (xy), \eqno(2.5.5.9)] where [ s = {\scr F}^{-1} [A (U)] \sin \chi]], which includes only an imaginary part of function (2.5.5.6)[link]. While selecting defocus in such a way that under the Scherzer defocus conditions [(2.5.2.44)[link], (2.5.2.45[link])] [|\sin \chi| \simeq 1], one could obtain [I (xy) = 1 + 2 \sigma \varphi (xy) * a (xy). \eqno(2.5.5.10)] In this very simple case the image reflects directly the structure of the object – the two-dimensional distribution of the projection of the potential convoluted with the spread function [ {a} = {\scr F}^{-1} A]. In this case, no image restoration is necessary. Contrast reversal may be achieved by a change of defocus.

At high resolution, this method enables one to obtain an image of projections of the atomic structure of crystals and defects in the atomic arrangement – vacancies, replacements by foreign atoms, amorphous structures and so on; at resolution worse than atomic one obtains images of dislocations as continuous lines, inserted phases, inclusions etc. (Cowley, 1981[link]). It is also possible to obtain images of thin biological crystals, individual molecules, biological macromolecules and their associations.

Image restoration. In the case just considered (2.5.5.10)[link], the projection of potential [\varphi (xy)], convoluted with the spread function, can be directly observed. In the general case (2.5.5.9)[link], when the aperture becomes larger, the contribution to image formation is made by large values of spatial frequencies U, in which the function sin χ oscillates, changing its sign. Naturally, this distorts the image just in the region of appropriate high resolution. However, if one knows the form of the function sin χ (2.5.5.7)[link], the true function [\varphi (xy)] can be restored.

This could be carried out experimentally if one were to place in the back focal plane of an objective lens a zone plate transmitting only one-sign regions of sin χ (Hoppe, 1971[link]). In this case, the information on [\varphi (xy)] is partly lost, but not distorted. To perform such a filtration in an electron microscope is a rather complicated task.

Another method is used (Erickson & Klug, 1971[link]). It consists of a Fourier transformation [ {\scr F}^{-1}] of the measured intensity distribution TQ (2.5.5.6)[link] and division of this transform, according to (2.5.5.7a[link],b[link]), by the phase function sin χ. This gives [{TQ \over \sin \chi} = Q (uv) A (U). \eqno(2.5.5.11a)] Then, the new Fourier transformation [ {\scr F} QA] yields (in the weak-phase-object approximation) the true distribution [\varphi (xy) * a (xy). \eqno(2.5.5.11b)] The function sin χ depending on defocus Δf should be known to perform this procedure. The transfer function can also be found from an electron micrograph (Thon, 1966[link]). It manifests itself in a circular image intensity modulation of an amorphous substrate or, if the specimen is crystalline, in the `noise' component of the image. The analogue method (optical Fourier transformation for obtaining the image [\sin \chi]) can be used (optical diffraction, see below); digitization and Fourier transformation can also be applied (Hoppe et al., 1973[link]).

The thin crystalline specimen implies that in the back focal objective lens plane the discrete kinematic amplitudes [\Phi_{hk}] are arranged and, by the above method, they are corrected and released from phase distortions introduced by the function sin χ (see below) (Unwin & Henderson, 1975[link]).

For the three-dimensional reconstruction (see Section 2.5.6[link]) it is necessary to have the projections of potential of the specimen tilted at different angles α to the beam direction (normal beam incidence corresponds to [\alpha = 0]). In this case, the defocus Δf changes linearly with increase of the distance l of specimen points from the rotation axis [\Delta f_{\alpha} = \Delta f_{0} (1 + l \sin \alpha)]. Following the above procedure for passing on to reciprocal space and correction of sin χ, one can find [\varphi_{\alpha} (xy)] (Henderson & Unwin, 1975[link]).

2.5.5.3. An account of absorption

| top | pdf |

Elastic interaction of an incident wave with a weak phase object is defined on its exit surface by the distribution of potential projection [\varphi (xy)]; however, in the general case, the electron scattering amplitude is a complex one (Glauber & Schomaker, 1953[link]). In such a way, the image itself has the phase and amplitude contrast. This may be taken into account if one considers not only the potential projection [\varphi (xy)], but also the `imaginary potential' [\mu (xy)] which describes phenomenologically the absorption in thin specimens. Then, instead of (2.5.5.5)[link], the wave on the exit surface of a specimen can be written as [q (xy) = 1 - i\sigma \varphi (xy) - \mu (xy) \eqno(2.5.5.12)] and in the back focal plane if [ \Phi = {\scr F}\varphi] and [ M = {\scr F}\mu] [Q (uv) = \delta (uv) - i\sigma \Phi (uv) - M (uv). \eqno(2.5.5.13)] Usually, μ is small, but it can, nevertheless, make a certain contribution to an image. In a sufficiently good linear approximation, it may be assumed that the real part cos χ of the phase function (2.5.5.7a)[link] affects [M(uv)], while [\Phi (xy)], as we know, is under the action of the imaginary part sin χ.

Thus, instead of (2.5.5.6)[link], one can write [Q (\exp i\chi) = \delta ({\bf u}) - i\sigma \Phi ({\bf u}) \sin \chi - M ({\bf u}) \cos \chi, \eqno(2.5.5.14)] and as the result, instead of (2.5.5.10)[link], [ \eqalignno{ I (xy) &= 1 + 2 \sigma \varphi (xy) * {\scr F}^{-1} (\sin \chi) * a (U) &\cr &\quad - 2 \mu (xy) * {\scr F}^{-1} (\cos \chi) * a (U). &(2.5.5.15)\cr}]

The functions [\varphi (xy)] and [\mu (xy)] can be separated by object imaging using the through-focus series method. In this case, using the Fourier transformation, one passes from the intensity distribution (2.5.5.15)[link] in real space to reciprocal space. Now, at two different defocus values [\Delta f_{1}] and [\Delta f_{2}] [(2.5.5.6[link]), (2.5.5.7a[link],b[link])] the values [\Phi ({\bf u})] and [M({\bf u})] can be found from the two linear equations (2.5.5.14)[link]. Using the inverse Fourier transformation, one can pass on again to real space which gives [\varphi ({\bf x})] and [\mu ({\bf x})] (Schiske, 1968[link]). In practice, it is possible to use several through-focus series and to solve a set of equations by the least-squares method.

Another method for processing takes into account the simultaneous presence of noise [N({\bf x})] and transfer function zeros (Kirkland et al., 1980[link]). In this method the space frequencies corresponding to small values of the transfer function modulus are suppressed, while the regions where such a modulus is large are found to be reinforced.

2.5.5.4. Thick crystals

| top | pdf |

When the specimen thickness exceeds a certain critical value ([\sim]50–100 Å), the kinematic approximation does not hold true and the scattering is dynamic. This means that on the exit surface of a specimen the wave is not defined as yet by the projection of potential [\varphi (xy) = {\textstyle\int} \varphi ({\bf r}) \hbox{ d}z] (2.5.5.3)[link], but one has to take into account the interaction of the incident wave [\psi_{0}] and of all the secondary waves arising in the whole volume of a specimen.

The dynamic scattering calculation can be made by various methods. One is the multi-slice (or phase-grating) method based on a recurrent application of formulae (2.5.5.3)[link] for n thin layers [\Delta z_{i}] thick, and successive construction of the transmission functions [q_{i}] (2.5.5.4)[link], phase functions [ Q_{i} = {\scr F}q_{i}], and propagation function [p_{k} = [k / 2\pi i \Delta z] \exp [ik (x^{2} + y^{2}) / 2 \Delta z]] (Cowley & Moodie, 1957[link]).

Another method – the scattering matrix method – is based on the solution of equations of the dynamic theory (Chapter 5.2[link] ). The emerging wave on the exit surface of a crystal is then found to diffract and experience the transfer function action [(2.5.5.6)[link], (2.5.5.7a[link],b[link])].

The dynamic scattering in crystals may be interpreted using Bloch waves: [\Psi\hskip 2pt^{j}({\bf r}) = {\textstyle\sum\limits_{H}} C^{\;j}_{H} \exp (-2\pi i{\bf k}^{\;j}_{H} \cdot {\bf r}). \eqno(2.5.5.16)] It turns out that only a few (bound and valence Bloch waves) have strong excitation amplitudes. Depending on the thickness of a crystal, only one of these waves or their linear combinations (Kambe, 1982[link]) emerges on the exit surface. An electron-microscopic image can be interpreted, at certain thicknesses, as an image of one of these waves [with a correction for the transfer function action (2.5.5.6)[link], (2.5.5.7a[link],b[link])]; in this case, the identical images repeat with increasing thickness, while, at a certain thickness, the contrast reversal can be observed. Only the first Bloch wave which arises at small thickness, and also repeats with increasing thickness, corresponds to the projection of potential [\varphi (xy)], i.e. the atom projection distribution in a thin crystal layer.

An image of other Bloch waves is defined by the function [\varphi ({\bf r})], but their maxima or minima do not coincide, in the general case, with the atomic positions and cannot be interpreted as the projection of potential. It is difficult to reconstruct [\varphi (xy)] from these images, especially when the crystal is not ideal and contains imperfections. In these cases one resorts to computer modelling of images at different thicknesses and defocus values, and to comparison with an experimentally observed pattern.

The imaging can be performed directly in an electron microscope not by a photo plate, but using fast-response detectors with digitized intensity output on line. The computer contains the necessary algorithms for Fourier transformation, image calculation, transfer function computing, averaging, and correction for the observed and calculated data. This makes possible the interpretation of the pattern observed directly in experiment (Herrmann et al., 1980[link]).

2.5.5.5. Image enhancement

| top | pdf |

The real electron-microscope image is subdivided into two components: [J(xy) = I(xy) + N(xy). \eqno(2.5.5.17)] The main of these, [I(xy)], is a two-dimensional image of the `ideal' object obtained in an electron microscope with instrumental functions inherent to it. However, in the process of object imaging and transfer of this information to the detector there are various sources of noise. In an electron microscope, these arise owing to emission-current and accelerating-voltage fluctuations, lens-supplying current (temporal fluctuations), or mechanical instabilities in a device, specimen or detector (spatial shifts). The two-dimensional detector (e.g. a photographic plate) has structural inhomogeneities affecting a response to the signal. In addition, the specimen is also unstable; during preparation or imaging it may change owing to chemical or some other transformations in its structure, thermal effects and so on. Biological specimens scatter electrons very weakly and their natural state is moist, while in the electron-microscope column they are under vacuum conditions. The methods of staining (negative or positive), e.g. of introducing into specimens substances containing heavy atoms, as well as the freeze-etching method, somewhat distort the structure of a specimen. Another source of structure perturbation is radiation damage, which can be eliminated at small radiation doses or by using the cryogenic technique. The structure of stained specimens is affected by stain graininess. We assume that all the deviations [\Delta I_{k}(xy)] of a specimen image from the `ideal' image [I_{k}(xy)] are included in the noise term [N_{k}(xy)]. The substrate may also be inhomogeneous. All kinds of perturbations cannot be separated and they appear on an electron microscope image as the full noise content [N(xy)].

The image enhancement involves maximum noise suppression [N(xy)] and hence the most accurate separation of a useful signal [I(xy)] from the real image [J(xy)] (2.5.5.1)[link]. At the signal/noise ratio [I/N \simeq 1] such a separation appears to be rather complicated. But in some cases the real image reflects the structure sufficiently well, e.g. during the atomic structure imaging of some crystals [(I/N \;\gt\; 10)]. In other cases, especially of biological specimen imaging, the noise N distorts substantially the image, [ (I/N) \sim 5\hbox{--} 10]. Here one should use the methods of enhancement. This problem is usually solved by the methods of statistical processing of sets of images [J_{k}] [(k = 1, \ldots, n)]. If one assumes that the informative signal [I_{k}(xy)] is always the same, then the noise error [N(xy)] may be reduced.

The image enhancement methods are subdivided into two classes:

  • (a) image averaging in real space xy;

  • (b) Fourier analysis and filtration in reciprocal space.

These methods can be used separately or in combination. The enhancement can be applied to both the original and the restored images; there are also methods of simultaneous restoration and enhancement.

The image can be enhanced by analogue (mainly optical and photographic) methods or by computational methods for processing digitized functions in real and reciprocal space.

The cases where the image has translational symmetry, rotational symmetry, and where the image is asymmetric will be considered.

Periodic images. An image of the crystal structure with atomic or molecular resolution may be brought to self-alignment by a shift by a and b periods in a structure projection. This can be performed photographically by printing the shifted image on the same photographic paper or, vice versa, by shifting the paper (McLachlan, 1958[link]).

The Fourier filtration method for a periodic image [I_{p}] with noise N is based on the fact that in Fourier space the components [ {\scr F}I_{p}] and [ {\scr F}N] are separated. Let us carry out the Fourier transformation of the periodic signal [I_{p}] with the periods a, b and noise N: [ \eqalignno{ {\scr F}J &= {\scr F}[I_{p}(xy) + N(xy)] &\cr &= {\textstyle\int} I_{p}(xy) \exp [2\pi i(hx + ky)]\;\hbox{d}x\;\hbox{d}y + {\scr F}N &\cr &= {\textstyle\sum} \Phi_{hk} \delta ({\bf u} - {\bf u}_{hk}) + {\scr F}N\hbox{;} &(2.5.5.18)\cr {\bf u}_{hk} &= h{\bf a}^{*} + k{\bf b}^{*}. &\cr}] The left part of (2.5.5.18)[link] represents the Fourier coefficients [\Phi_{hk}] distributed discretely with periods [a^{*}] and [b^{*}] in the plane [{\bf u}(uv)]. This is the two-dimensional reciprocal lattice. The right-hand side of (2.5.5.18)[link] is the Fourier transform [ {\scr F}N] distributed continuously in the plane. Thus these parts are separated. Let us `cut out' from distribution (2.5.5.18)[link] only [\Phi_{hk}] values using the `window' function [w(uv)]. The window should match each of the real peaks [\Phi_{hk}] which, owing to the finite dimensions of the initial periodic image, are not points, as this is written in an idealized form in (2.5.5.18)[link] with the aid of δ functions. In reality, the `windows' may be squares of about [a^{*}/10], [b^{*}/10] in size, or a circle. Performing the Fourier transformation of product (2.5.5.18)[link] without [ {\scr F}N], and set of windows [w({\bf u}) = w(uv) * {\textstyle\sum_{h, \, k}} \delta ({\bf u} - h{\bf a}^{*} - k{\bf b}^{*})], we obtain: [ \eqalignno{ J(xy) &= {\scr F}^{-1} \{w({\bf u}) {\textstyle\sum\limits_{h, \, k}} \Phi_{h, \, k} \delta ({\bf u} - {\bf u}_{h, \, k})\} &\cr &= W(xy) * I_{p}({\bf x}), &(2.5.5.19)\cr}] the periodic component without the background, [ W(xy) =] [{\scr F}^{-1} w({\bf u})]. The zero coefficient [\Phi_{00}] in (2.5.5.19)[link] should be decreased, since it is due, in part, to the noise. When the window w is sufficiently small, [I_{p}] in (2.5.5.19)[link] represents the periodic distribution [\langle I\rangle] (average over all the unit cells of the projection) included in [I_{p}] (2.5.5.18)[link]. Nevertheless, some error from noise in an image does exist, since with [\Phi_{hk}] we also introduced into the inverse Fourier transformation the background transform values [ {\scr F}^{-1} N_{hk}] which are within the `windows'.

This approach is realized by an analogue method [optical diffraction and filtering of electron micrographs in a laser beam (Klug & Berger, 1964[link])] and can also be carried out by computing.

As an example, Fig. 2.5.5.2(b)[link] shows an electron micrograph of the periodic structure of a two-dimensional protein crystal, while Fig. 2.5.5.2(c)[link] represents optical diffraction from this layer. In order to dissect the aperiodic component [ {\scr F}N] in a diffraction plane, according to the scheme in Fig. 2.5.5.2(a)[link], one places a mask with windows covering reciprocal-lattice points. After such a filtration, only the [I_{p}] component makes a contribution during the image formation by means of a lens, while the component [ {\scr F}N] diffracted by the background is delayed. As a result, an optical pattern of the periodic structure is obtained (Fig. 2.5.5.2d[link]).

[Figure 2.5.5.2]

Figure 2.5.5.2 | top | pdf |

(a) Diagram of an optical diffractometer. D is the object (an electron micrograph), [M_{p}] is the diffraction plane and a mask that transmits only [\Phi_{hk}], [D_{p}] is the plane of the (filtered) image; (b) an electron micrograph of a crystalline layer of the protein phosphorylase b; (c) its optical diffraction pattern (the circles correspond to the windows in the mask that transmits only the [\Phi_{hk}] diffracted beams from the periodic component of the image); (d) the filtered image. Parts (b)–(d) are based on the article by Kiselev et al. (1971)[link].

Optical diffractometry also assists in determining the parameters of a two-dimensional lattice and its symmetry.

Using the same method, one can separate the superimposed images of two-dimensional structures with different periodicity and in different orientation, the images of the `near' and `far' sides of tubular periodic structures with monomolecular walls (Klug & DeRosier, 1966[link]; Kiselev et al., 1971[link]), and so on.

Computer filtering involves measuring the image optical density [J_{\rm obs}], digitization, and Fourier transformation (Crowther & Amos, 1971[link]). The sampling distance usually corresponds to one-third of the image resolution. When periodic weak phase objects are investigated, the transformation (2.5.5.18)[link] yields the Fourier coefficients. If necessary, we can immediately make corrections in them using the microscope transfer function according to (2.5.5.6)[link], (2.5.5.7a,[link]b[link]) and (2.5.5.11a[link]), and thereby obtain the true kinematic amplitudes [\Phi_{hk}]. The inverse transformation (2.5.5.16)[link] gives a projection of the structure (Unwin & Henderson, 1975[link]; Henderson & Unwin, 1975[link]).

Sometimes, an observed image [J({\bf x})] is `noised' by the [N({\bf x})] to a great extent. Then, one may combine data on real and reciprocal space to construct a sufficiently accurate image. In this case, the electron-diffraction pattern is measured and structure-factor moduli from diffraction reflection intensities [I_{hk, \, {\rm obs}}] are obtained: [|\Phi_{hk, \, {\rm obs}}| \sim \sqrt{I_{hk, \, {\rm obs}}}. \eqno(2.5.5.20)] At the same time, the structure factors [\Phi_{hk, \, {\rm calc}} = |\Phi_{hk, \, {\rm calc}}| \exp (i\alpha_{hk, \, {\rm calc}}) \eqno(2.5.5.21)] are calculated from the processed structure projection image by means of the Fourier transformation. However, owing to poor image quality we take from these data only the values of phases [\alpha_{hk}] since they are less sensitive to scattering density distortions than the moduli, and construct the Fourier synthesis [\eqalignno{ I(xy) &= {\textstyle\sum\limits_{hk}} | \Phi_{hk, \, {\rm obs}}| \exp (i\alpha_{hk, \, {\rm calc}}) &\cr &\quad\times \exp [2\pi i(hx + ky)]. &(2.5.5.22)\cr}]

Here the possibilities of combining various methods open up, e.g. for obtaining the structure-factor moduli from X-ray diffraction, and phases from electron microscopy, and so on (Gurskaya et al., 1971[link]).

Images with point symmetry. If a projection of an object (and consequently, the object itself) has a rotational N-fold axis of symmetry, the structure coincides with itself on rotation through the angle [2\pi /N]. If the image is rotated through arbitrary angles and is aligned photographically with the initial image, then the best density coincidence will take place at a rotation through [\alpha = (k2\pi / N)\ (k = 1,\ldots, N)] which defines N. The pattern averaging over all the rotations will give the enhanced structure image with an [(N)^{1/2}] times reduced background (Markham et al., 1963[link]).

Rotational filtering can be performed on the basis of the Fourier expansion of an image in polar coordinates over the angles (Crowther & Amos, 1971[link]). [I(r, \psi) = {\textstyle\sum\limits_{n = -\infty}^{+\infty}} g_{n}(r) \exp (in\varphi). \eqno(2.5.5.23)] The integral over the radius from azimuthal components [g_{n}] gives their power