Tables for
Volume B
Reciprocal space
Edited by U. Shmueli

International Tables for Crystallography (2010). Vol. B, ch. 2.5, p. 381


P. A. Penczekg

Principle of random conical tilt reconstruction. A tilt pair of images of the same grid area is collected. By aligning the particle images in the untilted micrograph (left), the Eulerian angles of their counterparts in the tilted micrograph (right) are established. The particle images from the tilted micrograph are used for 3D reconstruction of the molecule (bottom). The set of projections form a cone in Fourier space; information within the cone remains undetermined.