International
Tables for Crystallography Volume C Mathematical, physical and chemical tables Edited by E. Prince © International Union of Crystallography 2006 
International Tables for Crystallography (2006). Vol. C, ch. 2.2, pp. 2629

The main book dealing with Laue geometry is Amorós, Buerger & Amorós (1975). This should be used in conjunction with Henry, Lipson & Wooster (1951), or McKie & McKie (1986); see also Helliwell (1992, chapter 7). There is a synergy between synchrotron and neutron Laue diffraction developments (see Helliwell & Wilkinson, 1994).
The single crystal is bathed in a polychromatic beam of Xrays containing wavelengths between λ_{min} and λ_{max}. A particular crystal plane will pick out a general wavelength λ for which constructive interference occurs and reflect according to Bragg's law where d is the interplanar spacing and is the angle of reflection. A sphere drawn with radius 1/λ and with the beam direction as diameter, passing through the origin of the reciprocal lattice (the point O in Fig. 2.2.1.1) , will yield a reflection in the direction drawn from the centre of the sphere and out through the reciprocallattice point (relp) provided the relp in question lies on the surface of the sphere. This sphere is known as the Ewald sphere. Fig. 2.2.1.1 shows the Laue geometry, in which there exists a nest of Ewald spheres of radii between 1/λ_{max} and 1/λ_{min}. An alternative convention is feasible whereby only a single Ewald sphere is drawn of radius 1 reciprocallattice unit (r.l.u.). Then each relp is no longer a point but a streak between λ_{min}/d and λ_{max}/d from the origin of reciprocal space (see McKie & McKie, 1986, p. 297). In the following discussions on the Laue approach, this notation is not followed. We use the nest of Ewald spheres of varying radii instead.
Any relp (hkl) lying in the region of reciprocal space between the 1/λ_{max} and 1/λ_{min} Ewald spheres and the resolution sphere 1/d_{min} will diffract (the shaded area in Fig. 2.2.1.1). This region of reciprocal space is referred to as the accessible or stimulated region. Fig. 2.2.1.2 shows a predicted Laue pattern from a well aligned protein crystal. For a description of the indexing of a Laue photograph, see Bragg (1928, pp. 28, 29).
For a Laue spot at a given , only the ratio λ/d is determined, whether it is a single or a multiple relp component spot. If the unitcell parameters are known from a monochromatic experiment, then a Laue spot at a given yields λ since d is then known. Conversely, precise unitcell lengths cannot be determined from a Laue pattern alone; methods are, however, being developed to determine these (see Carr, Cruickshank & Harding, 1992).
The main use of Laue photography has in the past been for adjustment of the crystal to a desired orientation. With smallmolecule crystals, the number of diffraction spots on a monochromatic photograph from a stationary crystal is very small. With unfiltered, polychromatic radiation, many more spots are observed and so the Laue photograph serves to give a better idea of the crystal orientation and setting prior to precession photography. With protein crystals, the monochromatic still is used for this purpose before data collection via an area detector. This is because the number of diffraction spots is large on a monochromatic still and in a proteincrystal Laue photograph the stimulated spots from the Bremsstrahlung continuum are generally very weak. Synchrotronradiation Laue photographs of protein crystals can be recorded with short exposure times. These patterns consist of a large number of diffraction spots.
Crystal setting via Laue photography usually involves trying to direct the Xray beam along a zone axis. Angular missetting angles in the spindle and arc are easily calculated from the formula where Δ is the distance (resolved into vertical and horizontal) from the beam centre to the centre of a circle of spots defining a zone axis and D is the crystaltofilm distance.
After suitable angular correction to the sample orientation, the Laue photograph will show a pronounced blank region at the centre of the film (see Fig. 2.2.1.2). This radius of the blank region is determined by the minimum wavelength in the beam and the magnitude of the reciprocallattice spacing parallel to the Xray beam (see Jeffery, 1958). For the case, for example, of the Xray beam perpendicular to the a*b* plane, then where and R is the radius of the blank region (see Fig. 2.2.1.2), and D is the crystaltoflatfilm distance. If λ_{min} is known then an approximate value of c, for example, can be estimated. The principal zone axes will give the largest radii for the central blank region.
In Laue geometry, several relp's can occur in a Laue spot or ray. The number of relp's in a given spot is called the multiplicity of the spot. The number of spots of a given multiplicity can be plotted as a histogram. This is known as the multiplicity distribution. The form of this distribution is dependent on the ratio λ_{max}/λ_{min}. The multiplicity distribution in Laue diffraction is considered in detail by Cruickshank, Helliwell & Moffat (1987).
Any relp nh, nk, nl (n integer) will be stimulated by a wavelength λ/n since d_{nhnknl} = d_{hkl}/n, i.e. However, d_{nhnknl} must be d_{min} as otherwise the reflection is beyond the sample resolution limit.
If h, k, l have no common integer divisor and if 2h, 2k, 2l is beyond the resolution limit, then the spot on the Laue diffraction photograph is a singlewavelength spot. The probability that h, k, l have no common integer divisor is Hence, for a relp where d_{min} d_{hkl} 2d_{min} there is a very high probability (83.2%) that the Laue spot will be recorded as a singlewavelength spot. Since this region of reciprocal space corresponds to 87.5% (i.e. 7/8) of the volume of reciprocal space within the resolution sphere then 0.875 × 0.832 = 72.8% is the probability for a relp to be recorded in a singlewavelength spot. According to W. L. Bragg, all Laue spots should be multiple. He reasoned that for each h, k, l there will always be a 2h, 2k, 2l etc. lying within the same Laue spot. However, as the resolution limit is increased to accommodate this many more relp's are added, for which their hkl's have no common divisor.
The above discussion holds for infinite bandwidth. The effect of a more experimentally realistic bandwidth is to increase the proportion of singlewavelength spots.
The number of relp's within the resolution sphere is where = 1/d_{min} and V* is the reciprocal unitcell volume.
The number of relp's within the wavelength band λ_{max} to λ_{min}, for , is (Moffat, Schildkamp, Bilderback & Volz, 1986) Note that the number of relp's stimulated in a 0.1 Å wavelength interval, for example between 0.1 and 0.2 Å, is the same as that between 1.1 and 1.2 Å, for example. A large number of relp's are stimulated at one orientation of the crystal sample.
The proportion of relp's within a sphere of small d* (i.e. at low resolution) actually stimulated is small. In addition, the probability of them being single is zero in the infinitebandwidth case and small in the finitebandwidth case. However, Laue geometry is an efficient way of measuring a large number of relp's between and as singlewavelength spots.
The above is a brief description of the overall multiplicity distribution. For a given relp, even of simple hkl values, lying on a ray of several relp's (multiples of hkl), a suitable choice of crystal orientation can yield a singlewavelength spot. Consider, for example, a spot of multiplicity 5. The outermost relp can be recorded at long wavelength with the inner relp's on the ray excluded since they need λ's greater than λ_{max} (Fig. 2.2.1.3 ). Alternatively, by rotating the sample, the innermost relp can be measured uniquely at short wavelength with the outer relp's excluded (they require λ's shorter than λ_{min}). Hence, in Laue geometry several orientations are needed to recover virtually all relp's as singles. The multiplicity distribution is shown in Fig. 2.2.1.4 as a function of λ_{max}/λ_{min} (with the corresponding values of δλ/λ_{mean}).
There is an interesting variation in the angular separations of Laue reflections that shows up in the spatial distributions of spots on a detector plane (Cruickshank, Helliwell & Moffat, 1991). There are two main aspects to this distribution, which are general and local. The general aspects refer to the diffraction pattern as a whole and the local aspects to reflections in a particular zone of diffraction spots.
The general features include the following. The spatial density of spots is everywhere proportional to 1/D^{2}, where D is the crystaltodetector distance, and to 1/V*, where V* is the reciprocalcell volume. There is also though a substantial variation in spatial density with diffraction angle ; a prominent maximum occurs at
Local aspects of these patterns particularly include the prominent conics on which Laue reflections lie. That is, the local spatial distribution is inherently onedimensional in character. Between multiple reflections (nodals), there is always at least one single and therefore nodals have a larger angular separation from their nearest neighbours. The blank area around a nodal in a Laue pattern (Fig. 2.2.1.2) has been noted by Jeffery (1958). The smallest angular separations, and therefore spatially overlapped cases, are associated with single Laue reflections. Thus, the reflections involved in energy overlaps – the multiples – form a set largely distinct, except at short crystaltodetector distances, from those involved in spatial overlaps, which are mostly singles (Helliwell, 1985).
From a knowledge of the form of the angular distribution, it is possible, e.g. from the gaps bordering conics, to estimate and λ_{min}. However, a development of this involving gnomonic projections can be even more effective (Cruickshank, Carr & Harding, 1992).
A useful means of transformation of the flatfilm Laue pattern is the gnomonic projection. This converts the pattern of spots lying on curved arcs to points lying on straight lines. The stereographic projection is also used. Fig. 2.2.1.5 shows the graphical relationships involved [taken from International Tables, Vol. II (Evans & Lonsdale, 1959)], for the case of a Laue pattern recorded on a plane film, between the incidentbeam direction SN, which is perpendicular to a film plane and the Laue spot L and its spherical, stereographic, and gnomonic points S_{p}, S_{t} and G and the stereographic projection S_{r} of the reflected beams. If the radius of the sphere of projection is taken equal to D, the crystaltofilm distance, then the planes of the gnomonic projection and of the film coincide. The lines producing the various projection poles for any given crystal plane are coplanar with the incident and reflected beams. The transformation equations are
References
Amorós, J. L., Buerger, M. J. & Amorós, M. C. (1975). The Laue method. New York: Academic Press.Bragg, W. H. (1928). An introduction to crystal structure analysis. London: Bell.
Carr, P. D., Cruickshank, D. W. J. & Harding, M. M. (1992). The determination of unitcell parameters from Laue diffraction patterns using their gnomonic projections. J. Appl. Cryst. 25, 294–308.
Cruickshank, D. W. J., Carr, P. D. & Harding, M. M. (1992). Estimation of d_{min}, λ_{min} and λ_{max} from the gnomonic projections of Laue patterns. J. Appl. Cryst. 25, 285–293.
Cruickshank, D. W. J., Helliwell, J. R. & Moffat, K. (1987). Multiplicity distribution of reflections in Laue diffraction. Acta Cryst. A43, 656–674.
Cruickshank, D. W. J., Helliwell, J. R. & Moffat, K. (1991). Angular distribution of reflections in Laue diffraction. Acta Cryst. A47, 352–373.
Evans, H. T. & Lonsdale, K. (1959). Diffraction geometry. International tables for Xray crystallography, Vol. II, p. 164. Birmingham: Kynoch Press.
Helliwell, J. R. (1985). Protein crystallography with synchrotron radiation. J. Mol. Struct. 130, 63–91.
Helliwell, J. R. (1992). Macromolecular crystallography with synchrotron radiation. Cambridge University Press.
Helliwell, J. R. & Wilkinson, C. (1994). Xray and neutron Laue diffraction. In Neutron and synchrotron radiation for condensed matter studies: applications to soft condensed matter and biology, Vol. III, edited by J. Baruchel, J. L. Hodeau, M. S. Lehmann, J. R. Regnard & C. Schlenker. Berlin: Springer Verlag.
Henry, N. F. M., Lipson, H. & Wooster, W. A. (1951). The interpretation of Xray diffraction photographs. London: Macmillan.
International Tables for Xray Crystallography (1959). Vol. II. Birmingham: Kynoch Press.
Jeffery, J. W. (1958). An investigation of the blank areas on Laue photographs round: 1. The direct beam, and 2. Reflections with simple indices. Z. Kristallogr. 110, 321–328.
McKie, D. & McKie, C. (1986). Essentials of crystallography. Oxford: Blackwell Scientific Publications.
Moffat, K., Schildkamp, W., Bilderback, D. H. & Volz, K. (1986). Laue diffraction from biological samples. Nucl. Instrum. Methods, A246, 617–623.