International
Tables for
Crystallography
Volume C
Mathematical, physical and chemical tables
Edited by E. Prince

International Tables for Crystallography (2006). Vol. C, ch. 3.1, p. 152

Table 3.1.1.4 

P. F. Lindleya

aESRF, Avenue des Martyrs, BP 220, F-38043 Grenoble CEDEX, France

Table 3.1.1.4| top | pdf |
Reservoir solutions for sparse-matrix sampling (Jancarik & Kim 1991[link])

No.SaltBufferPrecipitant
(% by mass)
1 0.02 M Ca chloride 0.1 M Acetate 30% MPD
2     0.4 M Na, K tartrate
3     0.4 M NH4 phosphate
4   0.1 M Tris 2.0 M NH4 sulfate
5 0.2 M Na citrate 0.1 M Hepes 40% MPD
6 0.2 M Mg chloride 0.1 M Tris 30% PEG 4000
7   0.1 M Cacodylate 1.4 M Na acetate
8 0.2 M Na citrate 0.1 M Cacodylate 30% 2-Propanol
9 0.2 M NH4 acetate 0.1 M Citrate 30% PEG 4000
10 0.2 M NH4 acetate 0.1 M Acetate 30% PEG 4000
11   0.1 M Citrate 1.0 M NH4 phosphate
12 0.2 M Mg chloride 0.1 M Hepes 30% 2-Propanol
13 0.2 M Na citrate 0.1 M Tris 30% PEG 400
14 0.2 M Ca chloride 0.1 M Hepes 28% PEG 400
15 0.2 M NH4 sulfate 0.1 M Cacodylate 30% PEG 8000
16   0.1 M Hepes 1.5 M Li sulfate
17 0.2 M Li sulfate 0.1 M Tris 30% PEG 4000
18 0.2 M Mg acetate 0.1 M Cacodylate 20% PEG 8000
19 0.2 M NH4 acetate 0.1 M Tris 30% 2-Propanol
20 0.2 M NH4 sulfate 0.1 M Acetate 25% PEG 4000
21 0.2 M Mg acetate 0.1 M Cacodylate 30% MPD
22 0.2 M Na acetate 0.1 M Tris 30% PEG 4000
23 0.2 M Mg chloride 0.1 M Hepes 30% PEG 400
24 0.2 M Ca chloride 0.1 M Acetate 20% 2-Propanol
25   0.1 M Imidazole 1.0 M Na acetate
26 0.2 M NH4 acetate 0.1 M Citrate 30% MPD
27 0.2 M Na citrate 0.1 M Hepes 20% 2-Propanol
28 0.2 M Na acetate 0.1 M Cacodylate 30% PEG 8000
29   0.1 M Hepes 0.8 M Na, K tartrate
30 0.2 M NH4 sulfate   30% PEG 8000
31 0.2 M NH4 sulfate   30% PEG 4000
32     2.0 M NH4 sulfate
33     4.0 M Na formate
34   0.1 M Acetate 2.0 M Na formate
35   0.1 M Hepes 1.6 M Na, K phosphate
36   0.1 M Tris 8% PEG 8000
37   0.1 M Acetate 8% PEG 4000
38   0.1 M Hepes 1.4 M Na citrate
39   0.1 M Hepes 2% PEG 400, 2.0 M Na sulfate
40   0.1 M Citrate 20% 2-Propanol + 20% PEG 4000
41   0.1 M Hepes 10% 2-Propanol + 20% PEG 4000
42 0.05 M K phosphate   20% PEG 8000
43     30% PEG 1500
44     0.2 M Mg formate
45 0.2 M Zn acetate 0.1 M Cacodylate 18% PEG 8000
46 0.2 M Ca acetate 0.1 M Cacodylate 18% PEG 8000
47   0.1 M Acetate 2.0 M NH4 sulfate
48   0.1 M Tris 2.0 M NH4 sulfate
49 1.0 M Li sulfate   2% PEG 8000
50 1.0 M Li sulfate   15% PEG 8000

Abbreviations: tris: 2-amino-2-(hydroxymethyl)-1,3-propanediol; hepes: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid. Buffers: Na acetate buffer, pH = 4.6; Na citrate buffer, pH = 5.6; Na cacodylate buffer, pH = 6.5; Na hepes buffer, pH = 7.5; tris/HCl buffer, pH = 8.5.