Tables for
Volume F
Crystallography of biological macromolecules
Edited by E. Arnold, D. M. Himmel and M. G. Rossmann

International Tables for Crystallography (2012). Vol. F, ch. 10.1, p. 247   | 1 | 2 |

Section 10.1.5. Concluding note

H. Hopea* and S. Parkinb

aDepartment of Chemistry, University of California, Davis, One Shields Ave, Davis, CA 95616–5295, USA, and bDepartment of Chemistry, University of Kentucky, Lexington, Kentucky, USA
Correspondence e-mail:

10.1.5. Concluding note

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With correctly functioning low-temperature equipment and appropriate techniques, a crystal can be maintained frost-free for the duration of a data-collection run. Formation of frost on the crystal indicates malfunction of the equipment, or operator error. The most likely cause is operator error, but faulty equipment cannot be ruled out. The techniques described here have been used for collecting thousands of data sets from ice-free crystals and crystal mounts. There is no reason to accept frost problems as an unavoidable part of cryocrystallography.

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