Tables for
Volume F
Crystallography of biological macromolecules
Edited by E. Arnold, D. M. Himmel and M. G. Rossmann

International Tables for Crystallography (2012). Vol. F, ch. 3.1, p. 86   | 1 | 2 |


S. H. Hughesa and A. M. Stockb*

aNational Cancer Institute, Frederick Cancer R&D Center, Frederick, MD 21702–1201, USA, and bCenter for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute and University of Medicine and Dentistry of New Jersey – Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854–5627, USA
Correspondence e-mail:


Protein purification strategy. Purification of proteins expressed at reasonably high levels typically requires only a limited number of chromatographic steps. Additional chromatography columns (indicated in brackets) can be included as necessary. Affinity chromatography can allow efficient purification of fusion proteins or proteins with well defined ligand-binding domains.