International
Tables for
Crystallography
Volume F
Crystallography of biological macromolecules
Edited by E. Arnold, D. M. Himmel and M. G. Rossmann

International Tables for Crystallography (2012). Vol. F, ch. 4.2, pp. 123-124

Table 4.2.1.1 

H. Michela*

aMax-Planck-Institut für Biophysik, Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt/Main, Germany
Correspondence e-mail: michel@mpibp-frankfurt.mpg.de

Table 4.2.1.1| top | pdf |
Compilation of membrane proteins with known structures, including crystallization conditions and key references for the structure determinations

This table is continuously updated and can be inspected at http://www.mpibp-frankfurt.mpg.de/michel/public/memprotstruct.html . The membrane proteins listed are divided into polytopic membrane proteins from inner membranes of bacteria and mitochondria (a), membrane proteins from the outer membrane of Gram-negative bacteria (b) and monotopic membrane proteins [(c); these are proteins that are only inserted into the membrane, but do not span it]. Within parts (a), (b) and (c) the membrane proteins are listed in chronological order of structure determination.

(a) Polytopic membrane proteins from inner membranes of bacteria and mitochondria.

Membrane proteinCrystallization conditions (detergent/additive/precipitating agent)Key references (and pdb reference code, if available)
Photosynthetic reaction centre    
 from Rhodopseudomonas viridis N,N-Dimethyldodecylamine-N-oxide/heptane-1,2,3-triol/ammonium sulfate [1], [2] (1PRC), [3], [4] (2PRC, 3PRC, 4PRC, 5PRC, 6PRC, 7PRC)
 from Rhodobacter sphaeroides N,N-Dimethyldodecylamine-N-oxide/heptane-1,2,3-triol/polyethylene glycol 4000 [5] (4RCR)
Octyl-β-D-glucopyranoside/polyethylene glycol 4000 [6] (2RCR)
N,N-Dimethyldodecylamine-N-oxide/heptane-1,2,3-triol, dioxane/potassium phosphate [7] (1PCR)
Octyl-β-D-glucopyranoside/benzamidine, heptane-1,2,3-triol/polyethylene glycol 4000 [8] (1AIG, 1AIJ)
Bacteriorhodopsin    
 from Halobacterium salinarium (Electron crystallography using naturally occuring two-dimensional crystals) [9] (1BRD), [10] (2BRD), [11] (1AT9)
(Type I crystal grown in lipidic cubic phases) [12] (1AP9), [13] (1BRX)
Octyl-β-D-glucopyranoside/benzamidine/sodium phosphate (epitaxic growth on benzamidine crystals) [14] (1BRR)
Light-harvesting complex II    
 from pea chloroplasts (Electron crystallography of two-dimensional crystals prepared from Triton X100 solubilized material) [15]
Light-harvesting complex 2    
 from Rhodopseudomonas acidophila Octyl-β-D-glucopyranoside/benzamidine/phosphate [16] (1KZU)
 from Rhodospirillum molischianum N,N-dimethylundecylamine-N-oxide/heptane-1,2,3-triol/ammonium sulfate [17] (1LGH)
Cytochrome c oxidase    
 from Paracoccus denitrificans,    
 four-subunit enzyme complexed with antibody Fv fragment Dodecyl-β-D-maltoside/polyethylene glycol monomethylether 2000 [18]
 two-subunit enzyme complexed with antibody Fv fragment Undecyl-β-D-maltoside/polyethylene glycol monomethylether 2000 [19] (1AR1)
 from bovine heart mitochondria Decyl-β-D-maltoside with some residual cholate/polyethylene glycol 4000 [20], [21] (1OCC), [22] (2OCC, 1OCR)
Cytochrome bc1 complex    
 from bovine heart mitochondria Decanoyl-N-methylglucamide or diheptanoyl phosphatidyl choline/polyethylene glycol 4000 [23] (1QRC), [24]
Octyl-β-D-glucopyranoside/polyethylene glycol 4000 [25]
Pure dodecyl-β-D-maltoside or mixture with methyl-6-O-(N-heptylcarbamoyl)-α-D-glucopyranoside/polyethylene glycol 4000 [26]
 from chicken heart mitochondria Octyl-β-D-glucopyranoside/polyethylene glycol 4000 [25] (1BCC, 3BCC)
Potassium channel    
 from Streptomyces lividans N,N-Dimethyldodecylamine/polyethylene glycol 400 [27] (1BL8)
Mechanosensitive ion channel    
 from Mycobacterium tuberculosis Dodecyl-β-D-maltoside/triethylene glycol [28]

(b) Membrane proteins from the outer membrane of Gram-negative bacteria and related proteins.

Membrane proteinCrystallization conditions (detergent/additive/precipitating agent)Key references (and pdb reference code, if available)
16-Stranded porins    
 from Rhodobacter capsulatus Octytetraoxyethylene/polyethylene glycol 600 [29] (2POR)
 OmpF and PhoE from Escherichia coli Mixture of n-octyl-2-hydroxyethylsulfoxide and octylpolyoxyethylene; or N,N-dimethyl­decylamine-N-oxide/polyethylene glycol 2000 [30] (1OPF, 1PHO), [31]
 from Rhodopseudomonas blastica Octyltetraoxyethylene/heptane-1,2,3-triol/polyethylene glycol 600 [32] (1PRN)
 from Paracoccus denitrificans Octyl-β-D-glucoside/polyethylene glycol 600 [33]
18-Stranded porins    
 maltoporin from Escherichia coli Mixture of decyl-β-D-maltoside and dodecylnonaoxyethylene/polyethylene glycol 2000 [34] (1MAL)
 maltoporin from Salmonella typhimurium Mixture of octyltetraoxyethylene and N,N-dimethylhexylamine-N-oxide/polyethylene glycol 1500 [35] (1MPR, 2MPR)
 sucrose-specific ScrY porin from Salmonella typhimurium Mixture of octyl-β-D-glucopyranoside and N,N-dimethylhexylamine-N-oxide/polyethylene glycol 2000 [36] (1AOS, 1AOT)
α-Haemolysin    
 from Staphylococcus aureus Octyl-β-D-glucopyranoside/ammonium sulfate, polyethylene glycol monomethylether 5000 [37] (7AHL)
Eight-stranded β-barrel membrane anchor    
 OmpA fragment from Escherichia coli Not yet available [38] (1BXW)
22-Stranded receptors    
 FhuA from Escherichia coli N,N-Dimethyldecylamine-N-oxide/inositol/polyethylene glycol monomethylether 2000 [39] (1FCP, 2FCP)
n-Octyl-2-hydroxyethylsulfoxide/polyethylene glycol 2000 [40] (1BY3, 1BY5)
 ferric enterobacterin receptor (FepA) from Escherichia coli N,N-dimethyldodecylamine-N-oxide/heptane-1,2,3-triol/polyethylene glycol 1000 [41] (1FEP)

(c) Proteins inserted into, but not crossing the membrane (`monotopic membrane proteins').

Membrane proteinCrystallization conditions (detergent/additive/precipitating agent)Key references (and pdb reference code, if available)
Prostaglandin H2 synthase 1    
 (cyclooxygenase 1) from sheep Octyl-β-D-glucopyranoside/polyethylene glycol 4000 [42] (1PRH)
Cyclooxygenase 2    
 from mouse Octyl-β-D-glucopyranoside/polyethylene glycol monomethylether 550 [43] (1CX2, 3PGH, 4COX, 5COX, 6COX)
 from man Octylpentaoxyethylene/polyethylene glycol 4000 [44]
Squalene cyclase    
 from Alicyclobacillus acidocaldarius Octyltetraoxyethylene/sodium citrate [45] (1SQC)

References: [1] Diesenhofer et al. (1985[link]); [2] Diesenhofer et al. (1995[link]); [3] Lancaster & Michel (1997[link]); [4] Lancaster & Michel (1999[link]); [5] Allen et al. (1987[link]); [6] Chang et al. (1991[link]); [7] Ermler et al. (1994[link]); [8] Stowell et al. (1997[link]); [9] Henderson et al. (1990[link]); [10] Grigorieff et al. (1996[link]); [11] Kimura et al. (1997[link]); [12] Pebay-Peyroula et al. (1997[link]); [13] Luecke et al. (1998[link]); [14] Essen et al. (1998[link]); [15] Kühlbrandt et al. (1994[link]); [16] McDermott et al. (1995[link]); [17] Koepke et al. (1996[link]); [18] Iwata et al. (1995[link]); [19] Ostermeier et al. (1997[link]); [20] Tsukihara et al. (1995[link]); [21] Tsukihara et al. (1996[link]); [22] Yoshikawa et al. (1998[link]); [23] Xia et al. (1997[link]); [24] Kim et al. (1998[link]); [25] Zhang et al. (1998[link]); [26] Iwata et al. (1998[link]); [27] Doyle et al. (1998[link]); [28] Chang et al. (1998[link]); [29] Weiss et al. (1991[link]); [30] Cowan et al. (1992[link]); [31] Cowan et al. (1995[link]); [32] Kreusch et al. (1994[link]); [33] Hirsch et al. (1997[link]); [34] Schirmer et al. (1995[link]); [35] Meyer et al. (1997[link]); [36] Forst et al. (1998[link]); [37] Song et al. (1996[link]); [38] Pautsch & Schulz (1998[link]); [39] Ferguson et al. (1998[link]); [40] Locher et al. (1998[link]); [41] Buchanan et al. (1999[link]); [42] Picot et al. (1994[link]); [43] Kurumbail et al. (1996[link]); [44] Luong et al. (1996[link]); [45] Wendt et al. (1997[link]).