Tables for
Volume F
Crystallography of biological macromolecules
Edited by E. Arnold, D. M. Himmel and M. G. Rossmann

International Tables for Crystallography (2012). Vol. F, ch. 4.3, p. 135   | 1 | 2 |


Z. S. Derewendaa*

aDepartment of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA 22908–0736, USA
Correspondence e-mail:


Two examples of proteins crystallized by the surface-entropy reduction (SER) method. (a) The RGSL domain of the PDZRhoGEF nucleotide-exchange factor (PDB code 1htj ; Longenecker, Lewis et al., 2001[link]); the yellow spheres show the alanines introduced by mutagenesis, which mediate an isologous crystal contact across a crystallographic twofold axis. (b) The crystal structure of EpsI complexed with EpsJ (PDB code 2ret ; Yanez et al., 2008[link]); the EpsI protein (pale blue) contains two surface mutations, shown by yellow spheres, which mediate heterologous crystal contacts.