International
Tables for
Crystallography
Volume F
Crystallography of biological macromolecules
Edited by E. Arnold, D. M. Himmel and M. G. Rossmann

International Tables for Crystallography (2012). Vol. F, ch. 9.1, p. 229   | 1 | 2 |

Section 9.1.17. Final remarks

Z. Dautera* and K. S. Wilsonb

aNCI Frederick & Argonne National Laboratory, Building 202, Argonne, IL 60439, USA, and bYork Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5YW, England
Correspondence e-mail:  dauter@anl.gov

9.1.17. Final remarks

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Optimal strategies for data collection are dependent on a number of factors. The alternative data-collection facilities to which access is potentially available, how long it takes to gain access and the overall time allocated all place restraints on the planning of the experiment. In view of this, it is not possible to provide absolute rules for optimal strategies.

Even after the source and overall time have been allocated or planned, the strategy is still the result of a compromise between several competing requirements. Some are general, others depend on the characteristics of a particular crystal or detector. As seen in the previous section, it is not possible to define protocols relevant for all applications. Rather, it is important to consider the relative importance of the parameters that can be varied to the problem in question and make the appropriate decisions.

Thus, data collection may have become easier from a technical point of view, but several crucial scientific decisions still have to be made by the experimenter. It is always beneficial to sacrifice some beam time and interpret the initial diffraction images, so as to avoid mistakes which may have an adverse effect on data quality and the whole of the subsequent structural analysis.








































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