International Tables for Crystallography

Use of SPIDER and SPIRE in image reconstruction
A. Leith, W. Baxter and J. Frank. International Tables for Crystallography (2012). Vol. F, ch. 19.8, pp. 620-623  [ doi:10.1107/97809553602060000874 ]


SPIDER is a comprehensive command-operated suite of programs for image processing in electron microscopy, with special emphasis on single-particle reconstruction. This chapter provides an outline of the way SPIDER and SPIRE, its interactive graphical user interface, may be used to obtain a three-dimensional reconstruction from projections of many randomly oriented realizations of a biological molecule.

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About International Tables for Crystallography

International Tables for Crystallography is the definitive resource and reference work for crystallography. The series consists of nine volumes and comprises articles and tables of data relevant to crystallographic research and to applications of crystallographic methods in all sciences concerned with the structure and properties of materials.