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The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering
International Tables for Crystallography (2012). Vol. F, ch. 12.1, pp. 317-326 [ doi:10.1107/97809553602060000837 ]
... 1954) and is often enhanced by anomalous scattering (MIRAS) [see Blundell & Johnson (1976) for a review]. The method remains popular ... with an amino acid modified by a heavy atom [see Blundell & Johnson (1976) for a review]. Alternatively, analogues of the ... extended in a comprehensive review of protein heavy-atom derivatives (Blundell & Johnson, 1976; Blundell & Jenkins, 1977) which analysed the dependence ...
Analogues of amino acids
International Tables for Crystallography (2012). Vol. F, Section 12.1.7, pp. 324-325 [ doi:10.1107/97809553602060000837 ]
... References Budisa, N., Karnbrock, W., Steinbacher, S., Humm, A., Prade, L., Neuefeind, T., Moroder, L. & Huber, R. (1997). Bioincorporation of telluromethionine into proteins: ...
Metal-ion replacement in metalloproteins
International Tables for Crystallography (2012). Vol. F, Section 12.1.6, p. 324 [ doi:10.1107/97809553602060000837 ]
Metal-ion replacement in metalloproteins 12.1.6. Metal-ion replacement in metalloproteins The metal-ion cofactor can sometimes be displaced by dialysis or diffusion by a heavy-atom solution, but usually the cofactor is first removed by a chelating agent (e.g. EDTA) or by acidification. These are best carried out on the ...
Polynuclear reagents
International Tables for Crystallography (2012). Vol. F, Section 12.1.5.7, p. 324 [ doi:10.1107/97809553602060000837 ]
... reviewed by Thygesen et al. (1996). References O'Halloran, T. V., Lippard, S. J., Richmond, T. J. & Klug, A. (1987). Multiple heavy-atom reagents for ...
[more results from section 12.1.5 in volume F]
Amino acids as ligands
International Tables for Crystallography (2012). Vol. F, Section 12.1.4, pp. 320-321 [ doi:10.1107/97809553602060000837 ]
... to give useful derivatives. References Ely, K. R., Girling, R. L., Schiffer, M., Cunningham, D. E. & Edmundson, A. B. (1973). ... nm resolution, a new study of the complex of glycyl-L-tyrosine with CPA, and mechanistic deductions. Philos. Trans. R. Soc. ...
Effect of concentration, time of soak and temperature on heavy-atom binding
International Tables for Crystallography (2012). Vol. F, Section 12.1.3.7, p. 320 [ doi:10.1107/97809553602060000837 ]
... times more slowly at 4°C than at ambient temperature (Blundell, 1968). A lower temperature allows greater control over the ... bind may do so upon elevation of the temperature. References Blundell, T. L. (1968). Unpublished results. Ringe, D., Petsko, G. ...
[more results from section 12.1.3 in volume F]
Heavy-atom data bank
International Tables for Crystallography (2012). Vol. F, Section 12.1.2, pp. 317-318 [ doi:10.1107/97809553602060000837 ]
Heavy-atom data bank 12.1.2. Heavy-atom data bank The heavy-atom data bank (HAD) is a computer-based archival file system that contains experimental and derived information from successful multiple isomorphous replacement analyses in the determination of protein crystal structures. HAD is available at http://www.sbg.bio.ic.ac.uk/had/ . The data ...
Introduction
International Tables for Crystallography (2012). Vol. F, Section 12.1.1, p. 317 [ doi:10.1107/97809553602060000837 ]
... 1954) and is often enhanced by anomalous scattering (MIRAS) [see Blundell & Johnson (1976) for a review]. The method remains popular ... with an amino acid modified by a heavy atom [see Blundell & Johnson (1976) for a review]. Alternatively, analogues of the ... extended in a comprehensive review of protein heavy-atom derivatives (Blundell & Johnson, 1976; Blundell & Jenkins, 1977) which analysed the dependence ...
Use of the heavy-atom data bank to select derivatives
International Tables for Crystallography (2012). Vol. F, Section 12.1.8, p. 325 [ doi:10.1107/97809553602060000837 ]
Use of the heavy-atom data bank to select derivatives 12.1.8. Use of the heavy-atom data bank to select derivatives The heavy-atom data bank is probably best exploited by first investigating the most commonly used heavy-atom reagents with a view to obtaining mercury, platinum and uranyl derivatives that ...
International Tables for Crystallography (2012). Vol. F, ch. 12.1, pp. 317-326 [ doi:10.1107/97809553602060000837 ]
... 1954) and is often enhanced by anomalous scattering (MIRAS) [see Blundell & Johnson (1976) for a review]. The method remains popular ... with an amino acid modified by a heavy atom [see Blundell & Johnson (1976) for a review]. Alternatively, analogues of the ... extended in a comprehensive review of protein heavy-atom derivatives (Blundell & Johnson, 1976; Blundell & Jenkins, 1977) which analysed the dependence ...
Analogues of amino acids
International Tables for Crystallography (2012). Vol. F, Section 12.1.7, pp. 324-325 [ doi:10.1107/97809553602060000837 ]
... References Budisa, N., Karnbrock, W., Steinbacher, S., Humm, A., Prade, L., Neuefeind, T., Moroder, L. & Huber, R. (1997). Bioincorporation of telluromethionine into proteins: ...
Metal-ion replacement in metalloproteins
International Tables for Crystallography (2012). Vol. F, Section 12.1.6, p. 324 [ doi:10.1107/97809553602060000837 ]
Metal-ion replacement in metalloproteins 12.1.6. Metal-ion replacement in metalloproteins The metal-ion cofactor can sometimes be displaced by dialysis or diffusion by a heavy-atom solution, but usually the cofactor is first removed by a chelating agent (e.g. EDTA) or by acidification. These are best carried out on the ...
Polynuclear reagents
International Tables for Crystallography (2012). Vol. F, Section 12.1.5.7, p. 324 [ doi:10.1107/97809553602060000837 ]
... reviewed by Thygesen et al. (1996). References O'Halloran, T. V., Lippard, S. J., Richmond, T. J. & Klug, A. (1987). Multiple heavy-atom reagents for ...
[more results from section 12.1.5 in volume F]
Amino acids as ligands
International Tables for Crystallography (2012). Vol. F, Section 12.1.4, pp. 320-321 [ doi:10.1107/97809553602060000837 ]
... to give useful derivatives. References Ely, K. R., Girling, R. L., Schiffer, M., Cunningham, D. E. & Edmundson, A. B. (1973). ... nm resolution, a new study of the complex of glycyl-L-tyrosine with CPA, and mechanistic deductions. Philos. Trans. R. Soc. ...
Effect of concentration, time of soak and temperature on heavy-atom binding
International Tables for Crystallography (2012). Vol. F, Section 12.1.3.7, p. 320 [ doi:10.1107/97809553602060000837 ]
... times more slowly at 4°C than at ambient temperature (Blundell, 1968). A lower temperature allows greater control over the ... bind may do so upon elevation of the temperature. References Blundell, T. L. (1968). Unpublished results. Ringe, D., Petsko, G. ...
[more results from section 12.1.3 in volume F]
Heavy-atom data bank
International Tables for Crystallography (2012). Vol. F, Section 12.1.2, pp. 317-318 [ doi:10.1107/97809553602060000837 ]
Heavy-atom data bank 12.1.2. Heavy-atom data bank The heavy-atom data bank (HAD) is a computer-based archival file system that contains experimental and derived information from successful multiple isomorphous replacement analyses in the determination of protein crystal structures. HAD is available at http://www.sbg.bio.ic.ac.uk/had/ . The data ...
Introduction
International Tables for Crystallography (2012). Vol. F, Section 12.1.1, p. 317 [ doi:10.1107/97809553602060000837 ]
... 1954) and is often enhanced by anomalous scattering (MIRAS) [see Blundell & Johnson (1976) for a review]. The method remains popular ... with an amino acid modified by a heavy atom [see Blundell & Johnson (1976) for a review]. Alternatively, analogues of the ... extended in a comprehensive review of protein heavy-atom derivatives (Blundell & Johnson, 1976; Blundell & Jenkins, 1977) which analysed the dependence ...
Use of the heavy-atom data bank to select derivatives
International Tables for Crystallography (2012). Vol. F, Section 12.1.8, p. 325 [ doi:10.1107/97809553602060000837 ]
Use of the heavy-atom data bank to select derivatives 12.1.8. Use of the heavy-atom data bank to select derivatives The heavy-atom data bank is probably best exploited by first investigating the most commonly used heavy-atom reagents with a view to obtaining mercury, platinum and uranyl derivatives that ...
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