International Tables for Crystallography

Use of SPIDER and SPIRE in image reconstruction
A. Leith, W. Baxter and J. Frank. International Tables for Crystallography (2012). Vol. F, ch. 19.8, pp. 620-623  [ doi:10.1107/97809553602060000874 ]


SPIDER is a comprehensive command-operated suite of programs for image processing in electron microscopy, with special emphasis on single-particle reconstruction. This chapter provides an outline of the way SPIDER and SPIRE, its interactive graphical user interface, may be used to obtain a three-dimensional reconstruction from projections of many randomly oriented realizations of a biological molecule.

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About International Tables for Crystallography

International Tables for Crystallography is the definitive resource and reference work for crystallography. The multi-volume series comprises articles and tables of data relevant to crystallographic research and to applications of crystallographic methods in all sciences concerned with the structure and properties of materials.